Experiment: Dimethyl Sulfoxide 6.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1356 and Pf1N1B4_1357 are separated by 135 nucleotides Pf1N1B4_1357 and Pf1N1B4_1358 are separated by 24 nucleotides Pf1N1B4_1358 and Pf1N1B4_1359 are separated by 297 nucleotides
Pf1N1B4_1356 - ATP-binding region, ATPase-like:Histidine kinase A, N-terminal, at 1,555,181 to 1,556,308
_1356
Pf1N1B4_1357 - Chaperone-modulator protein CbpM, at 1,556,444 to 1,556,749
_1357
Pf1N1B4_1358 - DnaJ-class molecular chaperone CbpA, at 1,556,774 to 1,557,721
_1358
Pf1N1B4_1359 - Putative heat shock protein YegD, at 1,558,019 to 1,559,293
_1359
Position (kb)
1556
1557
1558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1556.281 kb on + strand at 1556.282 kb on - strand at 1556.282 kb on - strand at 1556.727 kb on - strand at 1556.727 kb on - strand at 1556.727 kb on - strand at 1556.757 kb on + strand at 1556.757 kb on + strand at 1556.765 kb on - strand at 1556.765 kb on - strand at 1557.108 kb on + strand, within Pf1N1B4_1358 at 1557.109 kb on - strand, within Pf1N1B4_1358 at 1557.214 kb on + strand, within Pf1N1B4_1358 at 1557.214 kb on + strand at 1557.215 kb on - strand, within Pf1N1B4_1358 at 1557.215 kb on - strand, within Pf1N1B4_1358 at 1557.249 kb on + strand, within Pf1N1B4_1358 at 1557.250 kb on - strand, within Pf1N1B4_1358 at 1557.510 kb on + strand, within Pf1N1B4_1358 at 1557.510 kb on + strand, within Pf1N1B4_1358 at 1557.510 kb on + strand, within Pf1N1B4_1358 at 1557.564 kb on + strand, within Pf1N1B4_1358 at 1557.565 kb on - strand, within Pf1N1B4_1358 at 1557.627 kb on + strand at 1557.628 kb on - strand at 1557.628 kb on - strand at 1557.628 kb on - strand at 1557.628 kb on - strand at 1557.702 kb on + strand at 1557.703 kb on - strand at 1557.703 kb on - strand at 1557.703 kb on - strand at 1557.705 kb on + strand at 1557.705 kb on + strand at 1557.705 kb on + strand at 1557.705 kb on + strand at 1557.705 kb on + strand at 1557.706 kb on - strand at 1557.706 kb on - strand at 1557.706 kb on - strand at 1557.706 kb on - strand at 1557.885 kb on + strand at 1557.885 kb on + strand at 1557.886 kb on - strand at 1557.886 kb on - strand at 1557.886 kb on - strand at 1557.980 kb on + strand at 1557.980 kb on + strand at 1557.980 kb on + strand at 1557.980 kb on + strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1557.981 kb on - strand at 1558.461 kb on + strand, within Pf1N1B4_1359 at 1558.462 kb on - strand, within Pf1N1B4_1359 at 1558.532 kb on + strand, within Pf1N1B4_1359 at 1558.533 kb on - strand, within Pf1N1B4_1359 at 1558.533 kb on - strand, within Pf1N1B4_1359 at 1558.533 kb on - strand, within Pf1N1B4_1359
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6.5 vol% remove 1,556,281 + +0.2 1,556,282 - +0.1 1,556,282 - +1.2 1,556,727 - -0.8 1,556,727 - -0.8 1,556,727 - +0.2 1,556,757 + +0.5 1,556,757 + +0.2 1,556,765 - -0.5 1,556,765 - +0.2 1,557,108 + Pf1N1B4_1358 0.35 -1.6 1,557,109 - Pf1N1B4_1358 0.35 +0.2 1,557,214 + Pf1N1B4_1358 0.46 -1.6 1,557,214 + +0.2 1,557,215 - Pf1N1B4_1358 0.47 +0.5 1,557,215 - Pf1N1B4_1358 0.47 -0.8 1,557,249 + Pf1N1B4_1358 0.50 +0.6 1,557,250 - Pf1N1B4_1358 0.50 +0.2 1,557,510 + Pf1N1B4_1358 0.78 -2.0 1,557,510 + Pf1N1B4_1358 0.78 -1.4 1,557,510 + Pf1N1B4_1358 0.78 -0.8 1,557,564 + Pf1N1B4_1358 0.83 +0.2 1,557,565 - Pf1N1B4_1358 0.83 -1.0 1,557,627 + +0.2 1,557,628 - -0.8 1,557,628 - -1.4 1,557,628 - -1.7 1,557,628 - -2.1 1,557,702 + -0.3 1,557,703 - -3.1 1,557,703 - +1.5 1,557,703 - -0.3 1,557,705 + +0.8 1,557,705 + +0.2 1,557,705 + +0.6 1,557,705 + +0.2 1,557,705 + +1.2 1,557,706 - -2.1 1,557,706 - +1.2 1,557,706 - -0.5 1,557,706 - +0.8 1,557,885 + -0.2 1,557,885 + -0.0 1,557,886 - +0.2 1,557,886 - -2.3 1,557,886 - +1.9 1,557,980 + -0.4 1,557,980 + -1.4 1,557,980 + +1.2 1,557,980 + -2.1 1,557,981 - -0.4 1,557,981 - +1.2 1,557,981 - -0.5 1,557,981 - -2.6 1,557,981 - +0.2 1,557,981 - +1.2 1,557,981 - +1.2 1,558,461 + Pf1N1B4_1359 0.35 +0.8 1,558,462 - Pf1N1B4_1359 0.35 -0.8 1,558,532 + Pf1N1B4_1359 0.40 -0.4 1,558,533 - Pf1N1B4_1359 0.40 +0.7 1,558,533 - Pf1N1B4_1359 0.40 -0.8 1,558,533 - Pf1N1B4_1359 0.40 +1.8
Or see this region's nucleotide sequence