Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_166

Experiment: Doxycycline hyclate 0.000025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_165 and Ac3H11_166 are separated by 86 nucleotidesAc3H11_166 and Ac3H11_167 are separated by 144 nucleotides Ac3H11_165 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 50,864 to 52,006 _165 Ac3H11_166 - Glycine cleavage system H protein, at 52,093 to 52,467 _166 Ac3H11_167 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 52,612 to 55,524 _167 Position (kb) 52 53Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 51.264 kb on + strand, within Ac3H11_165at 51.264 kb on + strand, within Ac3H11_165at 51.264 kb on + strand, within Ac3H11_165at 51.264 kb on + strand, within Ac3H11_165at 51.264 kb on + strand, within Ac3H11_165at 51.264 kb on + strand, within Ac3H11_165at 51.265 kb on - strand, within Ac3H11_165at 51.265 kb on - strand, within Ac3H11_165at 51.450 kb on - strand, within Ac3H11_165at 51.593 kb on + strand, within Ac3H11_165at 51.853 kb on - strand, within Ac3H11_165at 51.887 kb on + strand, within Ac3H11_165at 51.887 kb on + strand, within Ac3H11_165at 51.887 kb on + strand, within Ac3H11_165at 51.887 kb on + strand, within Ac3H11_165at 51.888 kb on - strand, within Ac3H11_165at 52.046 kb on + strandat 52.047 kb on - strandat 52.047 kb on - strandat 52.047 kb on - strandat 52.047 kb on - strandat 52.063 kb on + strandat 52.064 kb on - strandat 52.068 kb on - strandat 52.068 kb on - strandat 52.288 kb on + strand, within Ac3H11_166at 52.288 kb on + strand, within Ac3H11_166at 52.288 kb on + strand, within Ac3H11_166at 52.288 kb on + strand, within Ac3H11_166at 52.288 kb on + strand, within Ac3H11_166at 52.288 kb on + strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.289 kb on - strand, within Ac3H11_166at 52.441 kb on + strandat 52.442 kb on - strandat 52.506 kb on + strandat 52.593 kb on - strandat 52.897 kb on + strandat 52.897 kb on + strandat 52.898 kb on - strandat 52.898 kb on - strandat 52.955 kb on - strand, within Ac3H11_167at 52.963 kb on + strand, within Ac3H11_167at 52.963 kb on + strand, within Ac3H11_167at 52.964 kb on - strand, within Ac3H11_167at 52.964 kb on - strand, within Ac3H11_167at 53.219 kb on + strand, within Ac3H11_167at 53.219 kb on + strand, within Ac3H11_167at 53.219 kb on + strand, within Ac3H11_167at 53.219 kb on + strand, within Ac3H11_167at 53.220 kb on - strand, within Ac3H11_167at 53.220 kb on - strand, within Ac3H11_167at 53.270 kb on - strand, within Ac3H11_167at 53.270 kb on - strand, within Ac3H11_167at 53.291 kb on - strand, within Ac3H11_167

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.000025 mg/ml
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51,264 + Ac3H11_165 0.35 +0.8
51,264 + Ac3H11_165 0.35 +0.2
51,264 + Ac3H11_165 0.35 -0.3
51,264 + Ac3H11_165 0.35 -0.1
51,264 + Ac3H11_165 0.35 +0.3
51,264 + Ac3H11_165 0.35 +0.1
51,265 - Ac3H11_165 0.35 -0.3
51,265 - Ac3H11_165 0.35 -0.5
51,450 - Ac3H11_165 0.51 +0.4
51,593 + Ac3H11_165 0.64 +0.6
51,853 - Ac3H11_165 0.87 +0.9
51,887 + Ac3H11_165 0.90 -1.5
51,887 + Ac3H11_165 0.90 +0.2
51,887 + Ac3H11_165 0.90 -1.4
51,887 + Ac3H11_165 0.90 +0.4
51,888 - Ac3H11_165 0.90 -0.4
52,046 + -0.2
52,047 - +0.3
52,047 - -0.1
52,047 - -0.0
52,047 - -0.5
52,063 + +0.5
52,064 - -2.0
52,068 - -0.3
52,068 - -1.8
52,288 + Ac3H11_166 0.52 +0.4
52,288 + Ac3H11_166 0.52 -0.1
52,288 + Ac3H11_166 0.52 -1.3
52,288 + Ac3H11_166 0.52 -0.3
52,288 + Ac3H11_166 0.52 +0.4
52,288 + Ac3H11_166 0.52 +0.1
52,289 - Ac3H11_166 0.52 +0.4
52,289 - Ac3H11_166 0.52 -1.6
52,289 - Ac3H11_166 0.52 +7.1
52,289 - Ac3H11_166 0.52 +0.7
52,289 - Ac3H11_166 0.52 +1.3
52,289 - Ac3H11_166 0.52 -1.1
52,441 + +0.4
52,442 - +0.2
52,506 + -0.6
52,593 - -0.0
52,897 + -1.0
52,897 + -0.9
52,898 - +1.2
52,898 - +0.2
52,955 - Ac3H11_167 0.12 +1.7
52,963 + Ac3H11_167 0.12 -0.2
52,963 + Ac3H11_167 0.12 -0.7
52,964 - Ac3H11_167 0.12 -0.3
52,964 - Ac3H11_167 0.12 -0.2
53,219 + Ac3H11_167 0.21 +1.7
53,219 + Ac3H11_167 0.21 -0.3
53,219 + Ac3H11_167 0.21 -0.6
53,219 + Ac3H11_167 0.21 +0.7
53,220 - Ac3H11_167 0.21 -0.4
53,220 - Ac3H11_167 0.21 -0.0
53,270 - Ac3H11_167 0.23 +0.5
53,270 - Ac3H11_167 0.23 -1.0
53,291 - Ac3H11_167 0.23 +1.5

Or see this region's nucleotide sequence