Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_673 and Pf1N1B4_674 are separated by 208 nucleotides Pf1N1B4_674 and Pf1N1B4_675 are separated by 854 nucleotides
Pf1N1B4_673 - MoxR-like ATPases, at 769,733 to 770,734
_673
Pf1N1B4_674 - POSSIBLE CONSERVED MEMBRANE PROTEIN, at 770,943 to 772,274
_674
Pf1N1B4_675 - hypothetical protein, at 773,129 to 773,347
_675
Position (kb) 770
771
772
773 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 770.031 kb on - strand, within Pf1N1B4_673 at 770.077 kb on + strand, within Pf1N1B4_673 at 770.078 kb on - strand, within Pf1N1B4_673 at 770.078 kb on - strand, within Pf1N1B4_673 at 770.246 kb on + strand, within Pf1N1B4_673 at 770.247 kb on - strand, within Pf1N1B4_673 at 770.466 kb on - strand, within Pf1N1B4_673 at 770.548 kb on - strand, within Pf1N1B4_673 at 770.548 kb on - strand, within Pf1N1B4_673 at 770.817 kb on - strand at 770.846 kb on + strand at 770.895 kb on - strand at 770.895 kb on - strand at 771.146 kb on + strand, within Pf1N1B4_674 at 771.147 kb on - strand at 771.147 kb on - strand, within Pf1N1B4_674 at 771.147 kb on - strand, within Pf1N1B4_674 at 771.147 kb on - strand, within Pf1N1B4_674 at 771.185 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.227 kb on - strand, within Pf1N1B4_674 at 771.394 kb on + strand, within Pf1N1B4_674 at 771.395 kb on - strand, within Pf1N1B4_674 at 771.421 kb on + strand at 771.421 kb on + strand, within Pf1N1B4_674 at 771.422 kb on - strand, within Pf1N1B4_674 at 771.422 kb on - strand, within Pf1N1B4_674 at 771.462 kb on + strand, within Pf1N1B4_674 at 771.462 kb on + strand at 771.639 kb on + strand, within Pf1N1B4_674 at 771.639 kb on + strand, within Pf1N1B4_674 at 771.639 kb on + strand at 771.640 kb on - strand, within Pf1N1B4_674 at 771.640 kb on - strand, within Pf1N1B4_674 at 771.759 kb on + strand, within Pf1N1B4_674 at 771.759 kb on + strand, within Pf1N1B4_674 at 771.759 kb on + strand at 771.876 kb on + strand, within Pf1N1B4_674 at 771.877 kb on - strand, within Pf1N1B4_674 at 771.909 kb on + strand at 771.931 kb on + strand, within Pf1N1B4_674 at 772.096 kb on + strand, within Pf1N1B4_674 at 772.122 kb on + strand, within Pf1N1B4_674 at 772.123 kb on - strand, within Pf1N1B4_674 at 772.123 kb on - strand, within Pf1N1B4_674 at 772.140 kb on + strand, within Pf1N1B4_674 at 772.140 kb on + strand, within Pf1N1B4_674 at 772.141 kb on - strand at 772.142 kb on + strand at 772.142 kb on + strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.150 kb on + strand at 772.150 kb on + strand at 772.150 kb on + strand at 772.243 kb on - strand at 772.244 kb on + strand at 772.244 kb on + strand at 772.245 kb on - strand at 772.272 kb on + strand at 772.272 kb on + strand at 772.273 kb on - strand at 772.287 kb on + strand at 772.294 kb on + strand at 772.295 kb on - strand at 772.309 kb on - strand at 772.314 kb on + strand at 772.314 kb on + strand at 772.315 kb on - strand at 772.315 kb on - strand at 772.315 kb on - strand at 772.331 kb on + strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.337 kb on + strand at 772.337 kb on + strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.355 kb on - strand at 772.359 kb on - strand at 772.359 kb on - strand at 772.388 kb on + strand at 772.388 kb on + strand at 772.395 kb on - strand at 772.395 kb on - strand at 772.428 kb on + strand at 772.429 kb on - strand at 772.485 kb on + strand at 772.597 kb on + strand at 772.651 kb on + strand at 772.652 kb on - strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.673 kb on - strand at 772.673 kb on - strand at 772.716 kb on + strand at 772.717 kb on - strand at 772.717 kb on - strand at 772.720 kb on - strand at 772.734 kb on - strand at 772.748 kb on - strand at 772.825 kb on - strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.922 kb on + strand at 773.066 kb on + strand at 773.067 kb on - strand at 773.086 kb on + strand at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 770,031 - Pf1N1B4_673 0.30 -0.4 770,077 + Pf1N1B4_673 0.34 -0.6 770,078 - Pf1N1B4_673 0.34 -1.0 770,078 - Pf1N1B4_673 0.34 -1.0 770,246 + Pf1N1B4_673 0.51 -1.6 770,247 - Pf1N1B4_673 0.51 -0.6 770,466 - Pf1N1B4_673 0.73 +0.0 770,548 - Pf1N1B4_673 0.81 -3.0 770,548 - Pf1N1B4_673 0.81 -0.6 770,817 - +1.0 770,846 + +0.0 770,895 - +0.0 770,895 - -2.3 771,146 + Pf1N1B4_674 0.15 +1.8 771,147 - +0.0 771,147 - Pf1N1B4_674 0.15 +0.0 771,147 - Pf1N1B4_674 0.15 +0.1 771,147 - Pf1N1B4_674 0.15 -0.4 771,185 - Pf1N1B4_674 0.18 +1.0 771,227 - Pf1N1B4_674 0.21 -1.0 771,227 - Pf1N1B4_674 0.21 -1.0 771,227 - Pf1N1B4_674 0.21 +0.4 771,227 - Pf1N1B4_674 0.21 -0.8 771,227 - Pf1N1B4_674 0.21 +0.0 771,227 - Pf1N1B4_674 0.21 +2.3 771,227 - Pf1N1B4_674 0.21 +1.7 771,394 + Pf1N1B4_674 0.34 +1.6 771,395 - Pf1N1B4_674 0.34 +0.9 771,421 + +1.0 771,421 + Pf1N1B4_674 0.36 +0.0 771,422 - Pf1N1B4_674 0.36 +1.8 771,422 - Pf1N1B4_674 0.36 +0.0 771,462 + Pf1N1B4_674 0.39 +0.9 771,462 + +0.0 771,639 + Pf1N1B4_674 0.52 +0.4 771,639 + Pf1N1B4_674 0.52 +0.9 771,639 + -1.0 771,640 - Pf1N1B4_674 0.52 +0.0 771,640 - Pf1N1B4_674 0.52 +0.7 771,759 + Pf1N1B4_674 0.61 +0.6 771,759 + Pf1N1B4_674 0.61 +1.0 771,759 + +1.8 771,876 + Pf1N1B4_674 0.70 -1.2 771,877 - Pf1N1B4_674 0.70 -1.0 771,909 + +0.0 771,931 + Pf1N1B4_674 0.74 -1.0 772,096 + Pf1N1B4_674 0.87 -1.0 772,122 + Pf1N1B4_674 0.89 -0.3 772,123 - Pf1N1B4_674 0.89 -0.7 772,123 - Pf1N1B4_674 0.89 -0.7 772,140 + Pf1N1B4_674 0.90 +1.0 772,140 + Pf1N1B4_674 0.90 +0.6 772,141 - +0.0 772,142 + +1.6 772,142 + +1.3 772,143 - -1.0 772,143 - -0.4 772,143 - +0.4 772,143 - +0.6 772,143 - +0.2 772,150 + +0.3 772,150 + +1.0 772,150 + -1.0 772,243 - -1.0 772,244 + -0.2 772,244 + +0.0 772,245 - -1.7 772,272 + +0.0 772,272 + +0.9 772,273 - -0.7 772,287 + -1.0 772,294 + +0.5 772,295 - -1.6 772,309 - -1.3 772,314 + -0.2 772,314 + -0.7 772,315 - +0.0 772,315 - +0.0 772,315 - +0.8 772,331 + -2.3 772,332 - -0.6 772,332 - -1.4 772,332 - +1.0 772,332 - +0.7 772,332 - +0.0 772,337 + -1.0 772,337 + +1.3 772,338 - -0.2 772,338 - +0.4 772,338 - -0.6 772,338 - +0.0 772,355 - +0.4 772,359 - +0.0 772,359 - +0.0 772,388 + -1.6 772,388 + +0.0 772,395 - +0.4 772,395 - +1.0 772,428 + -1.0 772,429 - -2.0 772,485 + +0.0 772,597 + +0.0 772,651 + -0.6 772,652 - +1.6 772,672 + -1.0 772,672 + +1.0 772,672 + -0.6 772,673 - +0.2 772,673 - -0.5 772,716 + +0.0 772,717 - +1.9 772,717 - -0.7 772,720 - -0.6 772,734 - -0.6 772,748 - -1.6 772,825 - -1.2 772,852 + +0.7 772,852 + +1.0 772,852 + -0.7 772,852 + +0.6 772,852 + +0.0 772,894 + +1.6 772,894 + -0.4 772,894 + +0.3 772,894 + +0.4 772,894 + -0.4 772,895 - +0.8 772,895 - -0.6 772,895 - +1.0 772,895 - -1.8 772,895 - +1.3 772,922 + -0.6 773,066 + -0.1 773,067 - +0.4 773,086 + +0.5 773,187 - Pf1N1B4_675 0.26 +0.6 773,187 - Pf1N1B4_675 0.26 +0.0 773,187 - Pf1N1B4_675 0.26 -0.3 773,274 - Pf1N1B4_675 0.66 +0.4 773,274 - Pf1N1B4_675 0.66 +1.3 773,274 - Pf1N1B4_675 0.66 +0.0
Or see this region's nucleotide sequence