Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_60 and Pf1N1B4_61 are separated by 276 nucleotides Pf1N1B4_61 and Pf1N1B4_62 are separated by 71 nucleotides Pf1N1B4_62 and Pf1N1B4_63 are separated by 200 nucleotides Pf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotides
Pf1N1B4_60 - Serine phosphatase RsbU, regulator of sigma subunit, at 64,018 to 65,199
_60
Pf1N1B4_61 - FIG00953170: hypothetical protein, at 65,476 to 65,775
_61
Pf1N1B4_62 - VacJ-like lipoprotein precursor, at 65,847 to 66,536
_62
Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357
_63
Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730
_64
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 65.279 kb on - strand at 65.315 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.319 kb on - strand at 65.325 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.434 kb on + strand at 65.434 kb on + strand at 65.434 kb on + strand at 65.497 kb on + strand at 65.498 kb on - strand at 65.754 kb on + strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.755 kb on - strand at 65.773 kb on + strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.600 kb on - strand at 66.600 kb on - strand at 66.619 kb on - strand at 66.707 kb on + strand at 66.716 kb on + strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.737 kb on - strand at 66.786 kb on + strand at 66.786 kb on + strand at 66.787 kb on - strand at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.991 kb on + strand at 66.992 kb on - strand at 66.992 kb on - strand, within Pf1N1B4_63 at 67.322 kb on - strand at 67.390 kb on - strand at 67.394 kb on + strand at 67.394 kb on + strand at 67.395 kb on - strand at 67.395 kb on - strand at 67.408 kb on - strand at 67.408 kb on - strand at 67.433 kb on + strand at 67.434 kb on - strand at 67.451 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 65,279 - -1.2 65,315 - -0.5 65,319 - -1.5 65,319 - -1.8 65,319 - -0.2 65,319 - +0.1 65,319 - +0.6 65,319 - +1.8 65,319 - -2.2 65,319 - +0.1 65,325 - -0.8 65,344 - -1.2 65,344 - -1.2 65,410 + -1.2 65,410 + -1.2 65,410 + +0.2 65,410 + +0.4 65,410 + -0.7 65,410 + -0.6 65,410 + +0.8 65,410 + +0.0 65,410 + +0.4 65,410 + +0.8 65,410 + -0.8 65,410 + +1.4 65,434 + +0.6 65,434 + -0.9 65,434 + -1.4 65,497 + +0.3 65,498 - +0.8 65,754 + -0.7 65,755 - +1.8 65,755 - -0.5 65,755 - -0.0 65,755 - -0.8 65,755 - +0.6 65,773 + -0.2 66,598 - -0.8 66,598 - -0.2 66,598 - +1.0 66,598 - -0.8 66,598 - -2.2 66,598 - +0.8 66,600 - -0.2 66,600 - +0.8 66,619 - -1.8 66,707 + -0.2 66,716 + -0.4 66,717 - +0.8 66,717 - -2.2 66,717 - -0.2 66,717 - -0.8 66,719 + -0.2 66,720 - +0.8 66,720 - -0.9 66,720 - +1.4 66,720 - -0.2 66,720 - -1.2 66,737 - -0.9 66,786 + +0.8 66,786 + +1.1 66,787 - +0.4 66,830 - Pf1N1B4_63 0.15 -1.2 66,830 - Pf1N1B4_63 0.15 +0.3 66,830 - Pf1N1B4_63 0.15 -1.2 66,991 + -0.2 66,992 - -0.2 66,992 - Pf1N1B4_63 0.41 -1.2 67,322 - -1.2 67,390 - -1.5 67,394 + -0.2 67,394 + +1.8 67,395 - -1.2 67,395 - -0.0 67,408 - -0.7 67,408 - -1.2 67,433 + -1.2 67,434 - -1.8 67,451 + -0.2
Or see this region's nucleotide sequence