Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides Pf1N1B4_5648 and Pf1N1B4_5649 are separated by 77 nucleotides Pf1N1B4_5649 and Pf1N1B4_5650 are separated by 175 nucleotides
Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927
_5647
Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465
_5648
Pf1N1B4_5649 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 76,543 to 77,766
_5649
Pf1N1B4_5650 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 77,942 to 79,588
_5650
Position (kb) 76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 75.577 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.626 kb on - strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.647 kb on - strand, within Pf1N1B4_5647 at 75.712 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 76.062 kb on + strand at 76.062 kb on + strand at 76.505 kb on - strand at 76.669 kb on + strand, within Pf1N1B4_5649 at 77.320 kb on + strand at 77.320 kb on + strand, within Pf1N1B4_5649 at 77.380 kb on + strand at 77.381 kb on - strand, within Pf1N1B4_5649 at 77.381 kb on - strand, within Pf1N1B4_5649 at 77.622 kb on + strand, within Pf1N1B4_5649 at 77.674 kb on - strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.719 kb on + strand at 77.743 kb on + strand at 77.760 kb on + strand at 77.761 kb on - strand at 77.761 kb on - strand at 77.765 kb on - strand at 77.765 kb on - strand at 77.919 kb on - strand at 78.050 kb on + strand at 78.079 kb on - strand at 78.087 kb on + strand at 78.088 kb on - strand at 78.150 kb on + strand, within Pf1N1B4_5650 at 78.150 kb on + strand, within Pf1N1B4_5650 at 78.657 kb on + strand, within Pf1N1B4_5650
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 75,577 + Pf1N1B4_5647 0.22 +0.2 75,625 + Pf1N1B4_5647 0.33 -1.4 75,625 + Pf1N1B4_5647 0.33 +0.4 75,625 + Pf1N1B4_5647 0.33 +1.2 75,626 - Pf1N1B4_5647 0.33 -0.6 75,627 + Pf1N1B4_5647 0.33 -0.8 75,627 + Pf1N1B4_5647 0.33 +0.2 75,627 + Pf1N1B4_5647 0.33 +0.4 75,627 + Pf1N1B4_5647 0.33 -0.0 75,627 + Pf1N1B4_5647 0.33 -2.7 75,647 - Pf1N1B4_5647 0.38 -0.4 75,712 + Pf1N1B4_5647 0.52 -0.9 75,869 + Pf1N1B4_5647 0.87 +0.4 75,869 + Pf1N1B4_5647 0.87 +1.6 76,062 + +0.4 76,062 + +2.9 76,505 - -0.6 76,669 + Pf1N1B4_5649 0.10 -2.0 77,320 + +0.2 77,320 + Pf1N1B4_5649 0.63 -1.2 77,380 + +0.6 77,381 - Pf1N1B4_5649 0.68 -0.0 77,381 - Pf1N1B4_5649 0.68 -3.0 77,622 + Pf1N1B4_5649 0.88 -2.0 77,674 - +0.6 77,719 + -0.4 77,719 + +0.6 77,719 + -0.0 77,719 + +0.7 77,743 + -1.0 77,760 + -0.4 77,761 - -1.2 77,761 - +1.2 77,765 - +1.9 77,765 - -1.2 77,919 - -0.4 78,050 + -1.0 78,079 - -0.4 78,087 + +0.4 78,088 - +1.2 78,150 + Pf1N1B4_5650 0.13 +1.0 78,150 + Pf1N1B4_5650 0.13 +0.8 78,657 + Pf1N1B4_5650 0.43 -0.4
Or see this region's nucleotide sequence