Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotides Pf1N1B4_5646 and Pf1N1B4_5647 are separated by 197 nucleotides Pf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides Pf1N1B4_5648 and Pf1N1B4_5649 are separated by 77 nucleotides
Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136
_5645
Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280
_5646
Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927
_5647
Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465
_5648
Pf1N1B4_5649 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 76,543 to 77,766
_5649
Position (kb) 75
76 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 74.523 kb on + strand, within Pf1N1B4_5645 at 74.524 kb on - strand at 74.584 kb on - strand, within Pf1N1B4_5645 at 74.656 kb on - strand, within Pf1N1B4_5645 at 74.721 kb on + strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.999 kb on - strand, within Pf1N1B4_5645 at 75.188 kb on - strand, within Pf1N1B4_5646 at 75.577 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.626 kb on - strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.647 kb on - strand, within Pf1N1B4_5647 at 75.712 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 76.062 kb on + strand at 76.062 kb on + strand at 76.505 kb on - strand at 76.669 kb on + strand, within Pf1N1B4_5649
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 74,523 + Pf1N1B4_5645 0.27 -2.0 74,524 - +0.6 74,584 - Pf1N1B4_5645 0.35 -1.8 74,656 - Pf1N1B4_5645 0.43 -0.4 74,721 + Pf1N1B4_5645 0.51 -2.7 74,722 - Pf1N1B4_5645 0.51 -2.4 74,722 - Pf1N1B4_5645 0.51 -1.4 74,999 - Pf1N1B4_5645 0.84 -0.0 75,188 - Pf1N1B4_5646 0.38 +2.9 75,577 + Pf1N1B4_5647 0.22 +0.2 75,625 + Pf1N1B4_5647 0.33 -1.4 75,625 + Pf1N1B4_5647 0.33 +0.4 75,625 + Pf1N1B4_5647 0.33 +1.2 75,626 - Pf1N1B4_5647 0.33 -0.6 75,627 + Pf1N1B4_5647 0.33 -0.8 75,627 + Pf1N1B4_5647 0.33 +0.2 75,627 + Pf1N1B4_5647 0.33 +0.4 75,627 + Pf1N1B4_5647 0.33 -0.0 75,627 + Pf1N1B4_5647 0.33 -2.7 75,647 - Pf1N1B4_5647 0.38 -0.4 75,712 + Pf1N1B4_5647 0.52 -0.9 75,869 + Pf1N1B4_5647 0.87 +0.4 75,869 + Pf1N1B4_5647 0.87 +1.6 76,062 + +0.4 76,062 + +2.9 76,505 - -0.6 76,669 + Pf1N1B4_5649 0.10 -2.0
Or see this region's nucleotide sequence