Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5589 and Pf1N1B4_5590 overlap by 4 nucleotides Pf1N1B4_5590 and Pf1N1B4_5591 are separated by 143 nucleotides
Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786
_5589
Pf1N1B4_5590 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 11,783 to 13,666
_5590
Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258
_5591
Position (kb) 11
12
13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 11.111 kb on + strand, within Pf1N1B4_5589 at 11.112 kb on - strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.314 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.541 kb on - strand, within Pf1N1B4_5589 at 11.542 kb on + strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.650 kb on - strand, within Pf1N1B4_5589 at 11.740 kb on + strand at 11.741 kb on - strand at 12.154 kb on - strand, within Pf1N1B4_5590 at 12.310 kb on - strand, within Pf1N1B4_5590 at 12.373 kb on + strand, within Pf1N1B4_5590 at 12.808 kb on - strand, within Pf1N1B4_5590 at 13.288 kb on - strand, within Pf1N1B4_5590 at 13.419 kb on + strand, within Pf1N1B4_5590 at 13.559 kb on + strand at 13.560 kb on - strand at 13.560 kb on - strand at 13.604 kb on + strand at 13.737 kb on + strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.965 kb on + strand, within Pf1N1B4_5591 at 14.184 kb on + strand, within Pf1N1B4_5591
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 11,111 + Pf1N1B4_5589 0.46 +0.9 11,112 - Pf1N1B4_5589 0.46 -0.9 11,295 + Pf1N1B4_5589 0.60 -0.0 11,295 + Pf1N1B4_5589 0.60 +0.5 11,314 + Pf1N1B4_5589 0.62 -2.0 11,383 + Pf1N1B4_5589 0.67 -0.4 11,383 + Pf1N1B4_5589 0.67 -2.4 11,541 - Pf1N1B4_5589 0.80 -0.4 11,542 + Pf1N1B4_5589 0.80 +1.2 11,543 - Pf1N1B4_5589 0.80 -1.0 11,543 - Pf1N1B4_5589 0.80 -1.4 11,650 - Pf1N1B4_5589 0.89 +0.2 11,740 + +0.3 11,741 - +1.6 12,154 - Pf1N1B4_5590 0.20 +0.2 12,310 - Pf1N1B4_5590 0.28 +1.6 12,373 + Pf1N1B4_5590 0.31 +0.8 12,808 - Pf1N1B4_5590 0.54 -0.1 13,288 - Pf1N1B4_5590 0.80 +1.2 13,419 + Pf1N1B4_5590 0.87 +0.3 13,559 + -0.4 13,560 - -1.0 13,560 - -0.4 13,604 + +1.4 13,737 + -0.1 13,744 - -1.4 13,744 - -0.1 13,744 - +0.6 13,744 - +0.6 13,744 - -0.4 13,965 + Pf1N1B4_5591 0.11 -1.0 14,184 + Pf1N1B4_5591 0.26 -2.0
Or see this region's nucleotide sequence