Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4085 and Pf1N1B4_4086 are separated by 135 nucleotides Pf1N1B4_4086 and Pf1N1B4_4087 are separated by 65 nucleotides Pf1N1B4_4087 and Pf1N1B4_4088 are separated by 122 nucleotides
Pf1N1B4_4085 - probable exported protein YPO0702, at 4,533,444 to 4,534,475
_4085
Pf1N1B4_4086 - transcriptional regulator, LuxR family, at 4,534,611 to 4,535,633
_4086
Pf1N1B4_4087 - MISCELLANEOUS; Unknown, at 4,535,699 to 4,536,073
_4087
Pf1N1B4_4088 - MISCELLANEOUS; Unknown, at 4,536,196 to 4,536,567
_4088
Position (kb)
4534
4535
4536 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4533.661 kb on + strand, within Pf1N1B4_4085 at 4533.662 kb on - strand, within Pf1N1B4_4085 at 4533.662 kb on - strand, within Pf1N1B4_4085 at 4533.771 kb on - strand, within Pf1N1B4_4085 at 4533.866 kb on - strand, within Pf1N1B4_4085 at 4533.948 kb on + strand, within Pf1N1B4_4085 at 4533.948 kb on + strand, within Pf1N1B4_4085 at 4533.949 kb on - strand, within Pf1N1B4_4085 at 4533.949 kb on - strand, within Pf1N1B4_4085 at 4534.053 kb on + strand, within Pf1N1B4_4085 at 4534.054 kb on - strand, within Pf1N1B4_4085 at 4534.069 kb on + strand, within Pf1N1B4_4085 at 4534.069 kb on + strand, within Pf1N1B4_4085 at 4534.069 kb on + strand, within Pf1N1B4_4085 at 4534.070 kb on - strand, within Pf1N1B4_4085 at 4534.070 kb on - strand, within Pf1N1B4_4085 at 4534.070 kb on - strand, within Pf1N1B4_4085 at 4534.174 kb on - strand, within Pf1N1B4_4085 at 4534.277 kb on + strand, within Pf1N1B4_4085 at 4534.371 kb on + strand at 4534.371 kb on + strand, within Pf1N1B4_4085 at 4534.371 kb on + strand, within Pf1N1B4_4085 at 4534.371 kb on + strand, within Pf1N1B4_4085 at 4534.372 kb on - strand at 4534.372 kb on - strand at 4534.451 kb on + strand at 4534.451 kb on + strand at 4534.678 kb on + strand at 4534.998 kb on + strand, within Pf1N1B4_4086 at 4534.999 kb on - strand, within Pf1N1B4_4086 at 4534.999 kb on - strand, within Pf1N1B4_4086 at 4534.999 kb on - strand, within Pf1N1B4_4086 at 4535.001 kb on + strand, within Pf1N1B4_4086 at 4535.001 kb on + strand, within Pf1N1B4_4086 at 4535.026 kb on + strand, within Pf1N1B4_4086 at 4535.232 kb on + strand, within Pf1N1B4_4086 at 4535.248 kb on + strand at 4535.478 kb on + strand, within Pf1N1B4_4086 at 4535.478 kb on + strand, within Pf1N1B4_4086 at 4535.479 kb on - strand, within Pf1N1B4_4086 at 4535.529 kb on + strand, within Pf1N1B4_4086 at 4535.529 kb on + strand, within Pf1N1B4_4086 at 4535.530 kb on - strand, within Pf1N1B4_4086 at 4535.642 kb on - strand at 4535.714 kb on - strand at 4535.714 kb on - strand at 4535.717 kb on - strand at 4535.761 kb on - strand, within Pf1N1B4_4087 at 4535.804 kb on - strand, within Pf1N1B4_4087 at 4535.842 kb on - strand at 4535.849 kb on + strand, within Pf1N1B4_4087 at 4535.850 kb on - strand, within Pf1N1B4_4087 at 4535.941 kb on - strand, within Pf1N1B4_4087 at 4535.941 kb on - strand, within Pf1N1B4_4087 at 4535.941 kb on - strand, within Pf1N1B4_4087 at 4535.941 kb on - strand, within Pf1N1B4_4087 at 4535.941 kb on - strand, within Pf1N1B4_4087 at 4535.975 kb on - strand, within Pf1N1B4_4087 at 4536.061 kb on - strand at 4536.061 kb on - strand at 4536.074 kb on - strand at 4536.074 kb on - strand at 4536.139 kb on - strand at 4536.200 kb on + strand at 4536.490 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.557 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 4,533,661 + Pf1N1B4_4085 0.21 -1.6 4,533,662 - Pf1N1B4_4085 0.21 -0.3 4,533,662 - Pf1N1B4_4085 0.21 +0.1 4,533,771 - Pf1N1B4_4085 0.32 -0.3 4,533,866 - Pf1N1B4_4085 0.41 -0.3 4,533,948 + Pf1N1B4_4085 0.49 -0.3 4,533,948 + Pf1N1B4_4085 0.49 -1.3 4,533,949 - Pf1N1B4_4085 0.49 +0.0 4,533,949 - Pf1N1B4_4085 0.49 +1.1 4,534,053 + Pf1N1B4_4085 0.59 +0.5 4,534,054 - Pf1N1B4_4085 0.59 -0.3 4,534,069 + Pf1N1B4_4085 0.61 -1.3 4,534,069 + Pf1N1B4_4085 0.61 +0.7 4,534,069 + Pf1N1B4_4085 0.61 +0.7 4,534,070 - Pf1N1B4_4085 0.61 -1.6 4,534,070 - Pf1N1B4_4085 0.61 +2.0 4,534,070 - Pf1N1B4_4085 0.61 -2.3 4,534,174 - Pf1N1B4_4085 0.71 +0.7 4,534,277 + Pf1N1B4_4085 0.81 +0.4 4,534,371 + +0.7 4,534,371 + Pf1N1B4_4085 0.90 +0.1 4,534,371 + Pf1N1B4_4085 0.90 +0.3 4,534,371 + Pf1N1B4_4085 0.90 +0.1 4,534,372 - +0.4 4,534,372 - -0.3 4,534,451 + +0.8 4,534,451 + +0.3 4,534,678 + -0.9 4,534,998 + Pf1N1B4_4086 0.38 -0.2 4,534,999 - Pf1N1B4_4086 0.38 -0.9 4,534,999 - Pf1N1B4_4086 0.38 -0.7 4,534,999 - Pf1N1B4_4086 0.38 -1.3 4,535,001 + Pf1N1B4_4086 0.38 +0.2 4,535,001 + Pf1N1B4_4086 0.38 +1.7 4,535,026 + Pf1N1B4_4086 0.41 -0.7 4,535,232 + Pf1N1B4_4086 0.61 +1.0 4,535,248 + +1.3 4,535,478 + Pf1N1B4_4086 0.85 +0.9 4,535,478 + Pf1N1B4_4086 0.85 -1.3 4,535,479 - Pf1N1B4_4086 0.85 -0.7 4,535,529 + Pf1N1B4_4086 0.90 +1.3 4,535,529 + Pf1N1B4_4086 0.90 -0.3 4,535,530 - Pf1N1B4_4086 0.90 +0.0 4,535,642 - -0.3 4,535,714 - +1.0 4,535,714 - +0.3 4,535,717 - +1.3 4,535,761 - Pf1N1B4_4087 0.17 +0.7 4,535,804 - Pf1N1B4_4087 0.28 +0.3 4,535,842 - -1.3 4,535,849 + Pf1N1B4_4087 0.40 +1.3 4,535,850 - Pf1N1B4_4087 0.40 +1.5 4,535,941 - Pf1N1B4_4087 0.65 +0.7 4,535,941 - Pf1N1B4_4087 0.65 +0.7 4,535,941 - Pf1N1B4_4087 0.65 -0.3 4,535,941 - Pf1N1B4_4087 0.65 -1.3 4,535,941 - Pf1N1B4_4087 0.65 -0.6 4,535,975 - Pf1N1B4_4087 0.74 +0.4 4,536,061 - -0.3 4,536,061 - -0.3 4,536,074 - +0.4 4,536,074 - +1.8 4,536,139 - -0.3 4,536,200 + -0.8 4,536,490 - Pf1N1B4_4088 0.79 -0.3 4,536,492 - Pf1N1B4_4088 0.80 -0.3 4,536,492 - Pf1N1B4_4088 0.80 +0.4 4,536,492 - -0.3 4,536,492 - Pf1N1B4_4088 0.80 -1.0 4,536,557 + -1.9
Or see this region's nucleotide sequence