Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3393 and Pf1N1B4_3394 are separated by 20 nucleotides Pf1N1B4_3394 and Pf1N1B4_3395 are separated by 270 nucleotides Pf1N1B4_3395 and Pf1N1B4_3396 are separated by 18 nucleotides Pf1N1B4_3396 and Pf1N1B4_3397 are separated by 141 nucleotides
Pf1N1B4_3393 - COGs COG3146, at 3,786,819 to 3,787,943
_3393
Pf1N1B4_3394 - Aquaporin Z, at 3,787,964 to 3,788,662
_3394
Pf1N1B4_3395 - Phosphohydrolase (MutT/nudix family protein), at 3,788,933 to 3,789,538
_3395
Pf1N1B4_3396 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,789,557 to 3,790,042
_3396
Pf1N1B4_3397 - Ketosteroid isomerase homolog, at 3,790,184 to 3,790,609
_3397
Position (kb)
3788
3789
3790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3787.953 kb on + strand at 3787.992 kb on + strand at 3787.992 kb on + strand at 3787.993 kb on - strand at 3787.993 kb on - strand at 3788.358 kb on + strand, within Pf1N1B4_3394 at 3788.358 kb on + strand, within Pf1N1B4_3394 at 3788.359 kb on - strand, within Pf1N1B4_3394 at 3788.359 kb on - strand, within Pf1N1B4_3394 at 3788.405 kb on - strand, within Pf1N1B4_3394 at 3788.405 kb on - strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.406 kb on + strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.407 kb on - strand, within Pf1N1B4_3394 at 3788.517 kb on - strand, within Pf1N1B4_3394 at 3788.517 kb on - strand, within Pf1N1B4_3394 at 3788.662 kb on + strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.684 kb on - strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.716 kb on - strand at 3788.736 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.793 kb on - strand at 3788.793 kb on - strand at 3788.820 kb on + strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.938 kb on - strand at 3788.945 kb on - strand at 3788.976 kb on + strand at 3788.976 kb on + strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3789.447 kb on + strand, within Pf1N1B4_3395 at 3789.504 kb on + strand at 3789.504 kb on + strand at 3789.505 kb on - strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.630 kb on + strand, within Pf1N1B4_3396 at 3789.631 kb on - strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.640 kb on - strand, within Pf1N1B4_3396 at 3790.021 kb on - strand at 3790.110 kb on + strand at 3790.146 kb on + strand at 3790.223 kb on + strand at 3790.224 kb on - strand at 3790.334 kb on + strand, within Pf1N1B4_3397
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 3,787,953 + +1.5 3,787,992 + -0.6 3,787,992 + -1.0 3,787,993 - -1.0 3,787,993 - +1.0 3,788,358 + Pf1N1B4_3394 0.56 +2.0 3,788,358 + Pf1N1B4_3394 0.56 +0.8 3,788,359 - Pf1N1B4_3394 0.57 +1.1 3,788,359 - Pf1N1B4_3394 0.57 +0.7 3,788,405 - Pf1N1B4_3394 0.63 +2.3 3,788,405 - Pf1N1B4_3394 0.63 -0.4 3,788,406 + Pf1N1B4_3394 0.63 +0.5 3,788,406 + Pf1N1B4_3394 0.63 -1.2 3,788,406 + -1.0 3,788,406 + Pf1N1B4_3394 0.63 -0.8 3,788,406 + Pf1N1B4_3394 0.63 +2.0 3,788,406 + Pf1N1B4_3394 0.63 +0.4 3,788,406 + Pf1N1B4_3394 0.63 -1.6 3,788,406 + Pf1N1B4_3394 0.63 -1.6 3,788,407 - Pf1N1B4_3394 0.63 -0.4 3,788,407 - Pf1N1B4_3394 0.63 +2.0 3,788,407 - Pf1N1B4_3394 0.63 +0.8 3,788,407 - Pf1N1B4_3394 0.63 -1.0 3,788,407 - Pf1N1B4_3394 0.63 +1.1 3,788,407 - Pf1N1B4_3394 0.63 +1.2 3,788,517 - Pf1N1B4_3394 0.79 +2.3 3,788,517 - Pf1N1B4_3394 0.79 +1.0 3,788,662 + +1.3 3,788,663 - -1.2 3,788,663 - -0.0 3,788,663 - +1.5 3,788,683 + +1.3 3,788,683 + -0.0 3,788,683 + -0.9 3,788,684 - -2.0 3,788,715 + -0.0 3,788,715 + +0.4 3,788,715 + -1.0 3,788,716 - +1.0 3,788,736 + -0.0 3,788,769 + -0.0 3,788,769 + -0.0 3,788,769 + -0.4 3,788,793 - -0.0 3,788,793 - +1.0 3,788,820 + -0.9 3,788,821 - +1.0 3,788,821 - +0.4 3,788,821 - +1.4 3,788,938 - -0.6 3,788,945 - -3.4 3,788,976 + -0.0 3,788,976 + +0.5 3,788,977 - -0.0 3,788,977 - -0.0 3,788,977 - +2.3 3,788,977 - +1.0 3,788,977 - +0.5 3,788,977 - -0.5 3,788,977 - -0.0 3,788,977 - -0.0 3,789,447 + Pf1N1B4_3395 0.85 +1.0 3,789,504 + +1.2 3,789,504 + -1.4 3,789,505 - -2.0 3,789,603 + -0.3 3,789,603 + -0.0 3,789,603 + +0.5 3,789,604 - -1.0 3,789,604 - -0.0 3,789,604 - -0.5 3,789,604 - +0.7 3,789,630 + Pf1N1B4_3396 0.15 -1.6 3,789,631 - Pf1N1B4_3396 0.15 -0.0 3,789,639 + Pf1N1B4_3396 0.17 -2.6 3,789,639 + Pf1N1B4_3396 0.17 +1.0 3,789,640 - Pf1N1B4_3396 0.17 -0.2 3,790,021 - -0.2 3,790,110 + +1.3 3,790,146 + +2.3 3,790,223 + -1.3 3,790,224 - -0.5 3,790,334 + Pf1N1B4_3397 0.35 -1.0
Or see this region's nucleotide sequence