Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2729 and Pf1N1B4_2730 are separated by 88 nucleotides Pf1N1B4_2730 and Pf1N1B4_2731 are separated by 178 nucleotides
Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587
_2729
Pf1N1B4_2730 - FIG00958173: hypothetical protein, at 3,069,676 to 3,070,350
_2730
Pf1N1B4_2731 - Uncharacterized protein conserved in bacteria, at 3,070,529 to 3,071,281
_2731
Position (kb)
3069
3070
3071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3068.732 kb on - strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.770 kb on + strand, within Pf1N1B4_2729 at 3068.771 kb on - strand, within Pf1N1B4_2729 at 3068.803 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3069.054 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.229 kb on + strand, within Pf1N1B4_2729 at 3069.386 kb on - strand, within Pf1N1B4_2729 at 3069.403 kb on + strand, within Pf1N1B4_2729 at 3069.403 kb on + strand, within Pf1N1B4_2729 at 3069.517 kb on + strand at 3069.517 kb on + strand at 3069.597 kb on + strand at 3069.598 kb on - strand at 3069.598 kb on - strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.605 kb on + strand at 3069.656 kb on + strand at 3069.816 kb on - strand, within Pf1N1B4_2730 at 3069.962 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.011 kb on - strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.333 kb on + strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.388 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.452 kb on - strand at 3070.503 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.518 kb on - strand at 3070.518 kb on - strand at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.973 kb on + strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3071.072 kb on - strand, within Pf1N1B4_2731 at 3071.191 kb on + strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.228 kb on + strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 3,068,732 - Pf1N1B4_2729 0.21 -0.3 3,068,747 + Pf1N1B4_2729 0.22 -0.2 3,068,747 + Pf1N1B4_2729 0.22 +0.3 3,068,747 + Pf1N1B4_2729 0.22 +0.3 3,068,747 + Pf1N1B4_2729 0.22 -0.7 3,068,747 + Pf1N1B4_2729 0.22 -0.1 3,068,747 + Pf1N1B4_2729 0.22 -1.3 3,068,748 - Pf1N1B4_2729 0.22 +1.3 3,068,748 - Pf1N1B4_2729 0.22 -1.1 3,068,748 - Pf1N1B4_2729 0.22 -0.2 3,068,748 - Pf1N1B4_2729 0.22 +1.3 3,068,748 - Pf1N1B4_2729 0.22 -0.7 3,068,748 - Pf1N1B4_2729 0.22 +0.3 3,068,748 - -0.7 3,068,748 - Pf1N1B4_2729 0.22 -0.1 3,068,748 - Pf1N1B4_2729 0.22 -0.6 3,068,748 - Pf1N1B4_2729 0.22 -0.6 3,068,770 + Pf1N1B4_2729 0.24 +1.3 3,068,771 - Pf1N1B4_2729 0.25 -0.5 3,068,803 + Pf1N1B4_2729 0.28 +0.8 3,068,866 + Pf1N1B4_2729 0.33 -1.1 3,068,866 + Pf1N1B4_2729 0.33 -3.1 3,068,866 + Pf1N1B4_2729 0.33 -0.7 3,068,866 + Pf1N1B4_2729 0.33 +0.3 3,068,866 + Pf1N1B4_2729 0.33 +0.3 3,068,866 + Pf1N1B4_2729 0.33 +0.3 3,068,867 - Pf1N1B4_2729 0.33 -2.7 3,068,867 - +0.3 3,068,867 - Pf1N1B4_2729 0.33 +0.3 3,068,867 - Pf1N1B4_2729 0.33 +0.3 3,068,867 - Pf1N1B4_2729 0.33 -0.7 3,069,054 + Pf1N1B4_2729 0.51 -0.4 3,069,124 + Pf1N1B4_2729 0.57 -0.4 3,069,124 + Pf1N1B4_2729 0.57 -0.5 3,069,229 + Pf1N1B4_2729 0.67 -1.3 3,069,386 - Pf1N1B4_2729 0.81 -0.6 3,069,403 + Pf1N1B4_2729 0.83 +0.8 3,069,403 + Pf1N1B4_2729 0.83 +1.8 3,069,517 + -0.7 3,069,517 + -1.7 3,069,597 + -0.1 3,069,598 - +1.3 3,069,598 - -0.0 3,069,599 + -2.1 3,069,599 + -2.1 3,069,599 + +1.8 3,069,600 - -1.7 3,069,600 - +1.3 3,069,600 - -1.3 3,069,605 + -0.3 3,069,656 + -0.7 3,069,816 - Pf1N1B4_2730 0.21 +1.3 3,069,962 + Pf1N1B4_2730 0.42 +0.3 3,070,010 + -0.7 3,070,010 + Pf1N1B4_2730 0.49 +1.3 3,070,010 + Pf1N1B4_2730 0.49 -1.3 3,070,010 + Pf1N1B4_2730 0.49 +0.2 3,070,010 + Pf1N1B4_2730 0.49 -1.7 3,070,011 - Pf1N1B4_2730 0.50 -0.2 3,070,163 + Pf1N1B4_2730 0.72 -0.1 3,070,163 + Pf1N1B4_2730 0.72 +0.8 3,070,163 + Pf1N1B4_2730 0.72 +0.3 3,070,163 + Pf1N1B4_2730 0.72 +0.3 3,070,163 + Pf1N1B4_2730 0.72 -0.5 3,070,163 + Pf1N1B4_2730 0.72 -0.7 3,070,164 - Pf1N1B4_2730 0.72 +0.3 3,070,164 - Pf1N1B4_2730 0.72 +0.3 3,070,164 - Pf1N1B4_2730 0.72 -0.9 3,070,164 - Pf1N1B4_2730 0.72 +0.3 3,070,164 - Pf1N1B4_2730 0.72 -1.3 3,070,164 - Pf1N1B4_2730 0.72 +0.4 3,070,165 + Pf1N1B4_2730 0.72 +1.3 3,070,165 + Pf1N1B4_2730 0.72 -0.2 3,070,192 + Pf1N1B4_2730 0.76 +0.1 3,070,192 + Pf1N1B4_2730 0.76 -0.5 3,070,193 - Pf1N1B4_2730 0.77 -1.3 3,070,193 - Pf1N1B4_2730 0.77 -0.3 3,070,193 - Pf1N1B4_2730 0.77 -1.7 3,070,193 - Pf1N1B4_2730 0.77 +0.3 3,070,316 + +2.3 3,070,316 + +0.7 3,070,316 + +0.3 3,070,317 - +2.3 3,070,317 - +0.3 3,070,317 - +0.8 3,070,317 - +0.3 3,070,317 - +0.3 3,070,317 - -0.4 3,070,333 + -0.3 3,070,334 - +1.3 3,070,334 - +0.3 3,070,334 - -0.3 3,070,334 - -1.1 3,070,334 - +0.3 3,070,334 - +0.3 3,070,334 - -0.7 3,070,334 - +0.3 3,070,334 - +0.7 3,070,388 + +0.5 3,070,418 + -0.1 3,070,418 + -0.3 3,070,418 + -0.6 3,070,418 + +0.3 3,070,419 - +1.3 3,070,419 - +0.7 3,070,419 - -1.3 3,070,452 - +0.3 3,070,503 + +1.3 3,070,517 + -0.7 3,070,517 + +0.3 3,070,517 + -1.2 3,070,517 + +0.3 3,070,517 + +0.3 3,070,518 - +0.9 3,070,518 - +0.3 3,070,665 - Pf1N1B4_2731 0.18 +0.5 3,070,665 - Pf1N1B4_2731 0.18 -0.7 3,070,973 + Pf1N1B4_2731 0.59 -0.3 3,070,974 - Pf1N1B4_2731 0.59 -0.7 3,070,974 - Pf1N1B4_2731 0.59 -1.7 3,070,974 - Pf1N1B4_2731 0.59 +1.3 3,070,974 - Pf1N1B4_2731 0.59 -0.5 3,071,072 - Pf1N1B4_2731 0.72 +0.3 3,071,191 + Pf1N1B4_2731 0.88 -0.6 3,071,192 - Pf1N1B4_2731 0.88 -1.3 3,071,192 - Pf1N1B4_2731 0.88 +0.3 3,071,192 - Pf1N1B4_2731 0.88 -0.4 3,071,228 + -1.0 3,071,229 - +2.7 3,071,229 - +1.0 3,071,229 - +0.4 3,071,230 + +0.8
Or see this region's nucleotide sequence