Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2192 and Pf1N1B4_2193 overlap by 4 nucleotides Pf1N1B4_2193 and Pf1N1B4_2194 are separated by 50 nucleotides Pf1N1B4_2194 and Pf1N1B4_2195 overlap by 1 nucleotides Pf1N1B4_2195 and Pf1N1B4_2196 are separated by 255 nucleotides
Pf1N1B4_2192 - Twin-arginine translocation protein TatB, at 2,503,926 to 2,505,059
_2192
Pf1N1B4_2193 - hypothetical protein, at 2,505,056 to 2,505,520
_2193
Pf1N1B4_2194 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 2,505,571 to 2,507,481
_2194
Pf1N1B4_2195 - Small-conductance mechanosensitive channel, at 2,507,481 to 2,508,062
_2195
Pf1N1B4_2196 - Homoserine/homoserine lactone efflux protein, at 2,508,318 to 2,508,950
_2196
Position (kb)
2505
2506
2507
2508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2504.720 kb on + strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.819 kb on + strand, within Pf1N1B4_2192 at 2504.820 kb on - strand, within Pf1N1B4_2192 at 2504.820 kb on - strand, within Pf1N1B4_2192 at 2504.974 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.057 kb on + strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.058 kb on - strand at 2505.236 kb on + strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.237 kb on - strand, within Pf1N1B4_2193 at 2505.345 kb on - strand, within Pf1N1B4_2193 at 2505.382 kb on + strand, within Pf1N1B4_2193 at 2505.382 kb on + strand, within Pf1N1B4_2193 at 2505.383 kb on - strand, within Pf1N1B4_2193 at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.483 kb on + strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.484 kb on - strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.520 kb on + strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.521 kb on - strand at 2505.526 kb on + strand at 2505.526 kb on + strand at 2505.526 kb on + strand at 2505.554 kb on + strand at 2505.570 kb on + strand at 2505.570 kb on + strand at 2505.596 kb on + strand at 2505.596 kb on + strand at 2505.597 kb on - strand at 2505.621 kb on - strand at 2505.817 kb on + strand, within Pf1N1B4_2194 at 2505.818 kb on - strand, within Pf1N1B4_2194 at 2505.818 kb on - strand, within Pf1N1B4_2194 at 2506.010 kb on + strand, within Pf1N1B4_2194 at 2506.010 kb on + strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.011 kb on - strand, within Pf1N1B4_2194 at 2506.147 kb on - strand, within Pf1N1B4_2194 at 2506.147 kb on - strand at 2506.217 kb on + strand, within Pf1N1B4_2194 at 2506.249 kb on + strand, within Pf1N1B4_2194 at 2506.315 kb on + strand, within Pf1N1B4_2194 at 2506.315 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.354 kb on + strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2506.355 kb on - strand, within Pf1N1B4_2194 at 2507.050 kb on - strand, within Pf1N1B4_2194 at 2507.050 kb on - strand, within Pf1N1B4_2194 at 2507.062 kb on + strand, within Pf1N1B4_2194 at 2507.063 kb on - strand, within Pf1N1B4_2194 at 2507.113 kb on + strand, within Pf1N1B4_2194 at 2507.114 kb on - strand, within Pf1N1B4_2194 at 2507.135 kb on - strand, within Pf1N1B4_2194 at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.335 kb on + strand at 2507.336 kb on - strand at 2507.568 kb on + strand, within Pf1N1B4_2195 at 2507.568 kb on + strand, within Pf1N1B4_2195 at 2507.569 kb on - strand at 2507.592 kb on - strand, within Pf1N1B4_2195 at 2507.828 kb on + strand, within Pf1N1B4_2195 at 2507.925 kb on + strand, within Pf1N1B4_2195 at 2507.926 kb on - strand, within Pf1N1B4_2195 at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.060 kb on + strand at 2508.214 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.278 kb on + strand at 2508.279 kb on - strand at 2508.283 kb on + strand at 2508.283 kb on + strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand at 2508.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,504,720 + Pf1N1B4_2192 0.70 -0.6 2,504,721 - Pf1N1B4_2192 0.70 -0.1 2,504,721 - Pf1N1B4_2192 0.70 -1.6 2,504,819 + Pf1N1B4_2192 0.79 +0.4 2,504,819 + Pf1N1B4_2192 0.79 -0.4 2,504,819 + Pf1N1B4_2192 0.79 -0.4 2,504,819 + Pf1N1B4_2192 0.79 +1.0 2,504,820 - Pf1N1B4_2192 0.79 -0.0 2,504,820 - Pf1N1B4_2192 0.79 -0.6 2,504,974 + +1.0 2,505,057 + -1.6 2,505,057 + +0.8 2,505,057 + +0.4 2,505,057 + +0.1 2,505,058 - +0.7 2,505,058 - -1.7 2,505,058 - -0.6 2,505,058 - -1.6 2,505,058 - -0.1 2,505,236 + Pf1N1B4_2193 0.39 -0.6 2,505,237 - Pf1N1B4_2193 0.39 +0.4 2,505,237 - Pf1N1B4_2193 0.39 +0.1 2,505,237 - Pf1N1B4_2193 0.39 -0.9 2,505,345 - Pf1N1B4_2193 0.62 -1.6 2,505,382 + Pf1N1B4_2193 0.70 -0.8 2,505,382 + Pf1N1B4_2193 0.70 +0.0 2,505,383 - Pf1N1B4_2193 0.70 +0.2 2,505,483 + -0.6 2,505,483 + +0.2 2,505,483 + +0.9 2,505,483 + +0.0 2,505,483 + -0.6 2,505,483 + +0.4 2,505,483 + -0.2 2,505,483 + +0.4 2,505,483 + +1.0 2,505,483 + -0.6 2,505,483 + -0.8 2,505,483 + -1.6 2,505,483 + -0.6 2,505,484 - -2.1 2,505,484 - -1.9 2,505,484 - -0.9 2,505,484 - -0.6 2,505,520 + -1.6 2,505,520 + -0.4 2,505,520 + +0.4 2,505,520 + -0.1 2,505,520 + +0.1 2,505,520 + -0.1 2,505,520 + -0.6 2,505,520 + -1.6 2,505,520 + +0.9 2,505,520 + -0.6 2,505,520 + -2.0 2,505,520 + +0.4 2,505,520 + +1.3 2,505,521 - -0.3 2,505,521 - -0.3 2,505,521 - +1.8 2,505,521 - -1.2 2,505,521 - +0.9 2,505,521 - +0.4 2,505,521 - -0.6 2,505,521 - +0.9 2,505,521 - -1.1 2,505,521 - +0.8 2,505,521 - -1.1 2,505,521 - +1.4 2,505,521 - +2.4 2,505,521 - +0.4 2,505,521 - -0.1 2,505,521 - -1.1 2,505,526 + -0.3 2,505,526 + +1.4 2,505,526 + -0.1 2,505,554 + +0.6 2,505,570 + -1.1 2,505,570 + -1.6 2,505,596 + -1.0 2,505,596 + +0.0 2,505,597 - -0.6 2,505,621 - -2.1 2,505,817 + Pf1N1B4_2194 0.13 -0.9 2,505,818 - Pf1N1B4_2194 0.13 +0.9 2,505,818 - Pf1N1B4_2194 0.13 +0.4 2,506,010 + Pf1N1B4_2194 0.23 -0.1 2,506,010 + Pf1N1B4_2194 0.23 +0.5 2,506,011 - Pf1N1B4_2194 0.23 -0.5 2,506,011 - Pf1N1B4_2194 0.23 +1.2 2,506,011 - Pf1N1B4_2194 0.23 +0.9 2,506,147 - Pf1N1B4_2194 0.30 -1.5 2,506,147 - +0.5 2,506,217 + Pf1N1B4_2194 0.34 -0.3 2,506,249 + Pf1N1B4_2194 0.35 +0.5 2,506,315 + Pf1N1B4_2194 0.39 +0.9 2,506,315 + Pf1N1B4_2194 0.39 -0.1 2,506,354 + Pf1N1B4_2194 0.41 +0.9 2,506,354 + Pf1N1B4_2194 0.41 +0.5 2,506,354 + Pf1N1B4_2194 0.41 -0.5 2,506,354 + Pf1N1B4_2194 0.41 +1.5 2,506,355 - Pf1N1B4_2194 0.41 +0.1 2,506,355 - Pf1N1B4_2194 0.41 -0.2 2,506,355 - Pf1N1B4_2194 0.41 -0.5 2,506,355 - Pf1N1B4_2194 0.41 -1.5 2,507,050 - Pf1N1B4_2194 0.77 +1.3 2,507,050 - Pf1N1B4_2194 0.77 +0.0 2,507,062 + Pf1N1B4_2194 0.78 +0.8 2,507,063 - Pf1N1B4_2194 0.78 +0.5 2,507,113 + Pf1N1B4_2194 0.81 +0.5 2,507,114 - Pf1N1B4_2194 0.81 +2.3 2,507,135 - Pf1N1B4_2194 0.82 +1.0 2,507,335 + +1.0 2,507,335 + -0.5 2,507,335 + -0.1 2,507,335 + -0.5 2,507,336 - -1.1 2,507,568 + Pf1N1B4_2195 0.15 +0.5 2,507,568 + Pf1N1B4_2195 0.15 -0.3 2,507,569 - +0.5 2,507,592 - Pf1N1B4_2195 0.19 +0.5 2,507,828 + Pf1N1B4_2195 0.60 -0.1 2,507,925 + Pf1N1B4_2195 0.76 +0.3 2,507,926 - Pf1N1B4_2195 0.76 -1.4 2,508,060 + -1.1 2,508,060 + +0.5 2,508,060 + -0.3 2,508,060 + +2.0 2,508,214 + -1.1 2,508,278 + +1.6 2,508,278 + -2.4 2,508,278 + -0.9 2,508,278 + +0.5 2,508,279 - -1.4 2,508,283 + +0.7 2,508,283 + -0.5 2,508,284 - +0.5 2,508,284 - -0.4 2,508,284 - +1.5 2,508,284 - +0.5 2,508,284 - -0.5
Or see this region's nucleotide sequence