Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_868 and Ac3H11_869 are separated by 227 nucleotides Ac3H11_869 and Ac3H11_870 are separated by 129 nucleotides Ac3H11_870 and Ac3H11_871 are separated by 18 nucleotides Ac3H11_871 and Ac3H11_872 overlap by 50 nucleotides
Ac3H11_868 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 150,167 to 151,366
_868
Ac3H11_869 - Auxin Efflux Carrier, at 151,594 to 152,547
_869
Ac3H11_870 - RNA-binding protein, at 152,677 to 153,012
_870
Ac3H11_871 - hypothetical protein, at 153,031 to 153,180
_871
Ac3H11_872 - FIG00581907: hypothetical protein, at 153,131 to 153,370
_872
Position (kb) 151
152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.594 kb on - strand, within Ac3H11_868 at 150.701 kb on + strand, within Ac3H11_868 at 150.716 kb on + strand, within Ac3H11_868 at 150.717 kb on - strand, within Ac3H11_868 at 150.845 kb on + strand, within Ac3H11_868 at 150.845 kb on + strand, within Ac3H11_868 at 150.845 kb on + strand, within Ac3H11_868 at 150.845 kb on + strand, within Ac3H11_868 at 150.845 kb on + strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.846 kb on - strand, within Ac3H11_868 at 150.851 kb on + strand, within Ac3H11_868 at 150.851 kb on + strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 150.852 kb on - strand, within Ac3H11_868 at 151.049 kb on + strand, within Ac3H11_868 at 151.050 kb on - strand, within Ac3H11_868 at 151.050 kb on - strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.115 kb on + strand, within Ac3H11_868 at 151.116 kb on - strand, within Ac3H11_868 at 151.116 kb on - strand, within Ac3H11_868 at 151.116 kb on - strand, within Ac3H11_868 at 151.127 kb on + strand, within Ac3H11_868 at 151.127 kb on + strand, within Ac3H11_868 at 151.127 kb on + strand, within Ac3H11_868 at 151.127 kb on + strand, within Ac3H11_868 at 151.127 kb on + strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.128 kb on - strand, within Ac3H11_868 at 151.205 kb on + strand, within Ac3H11_868 at 151.205 kb on + strand, within Ac3H11_868 at 151.205 kb on + strand, within Ac3H11_868 at 151.206 kb on - strand, within Ac3H11_868 at 151.206 kb on - strand, within Ac3H11_868 at 151.206 kb on - strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.208 kb on + strand, within Ac3H11_868 at 151.209 kb on - strand, within Ac3H11_868 at 151.209 kb on - strand, within Ac3H11_868 at 151.209 kb on - strand, within Ac3H11_868 at 151.209 kb on - strand, within Ac3H11_868 at 151.209 kb on - strand, within Ac3H11_868 at 151.226 kb on + strand, within Ac3H11_868 at 151.226 kb on + strand, within Ac3H11_868 at 151.226 kb on + strand, within Ac3H11_868 at 151.226 kb on + strand, within Ac3H11_868 at 151.227 kb on - strand, within Ac3H11_868 at 151.227 kb on - strand, within Ac3H11_868 at 151.319 kb on + strand at 151.418 kb on - strand at 151.418 kb on - strand at 151.426 kb on + strand at 151.426 kb on + strand at 151.427 kb on - strand at 151.427 kb on - strand at 151.427 kb on - strand at 151.452 kb on + strand at 151.452 kb on + strand at 151.452 kb on + strand at 151.452 kb on + strand at 151.452 kb on + strand at 151.453 kb on - strand at 151.453 kb on - strand at 151.453 kb on - strand at 151.453 kb on - strand at 151.453 kb on - strand at 151.456 kb on + strand at 151.456 kb on + strand at 151.456 kb on + strand at 151.499 kb on + strand at 151.586 kb on + strand at 151.586 kb on + strand at 151.586 kb on + strand at 151.586 kb on + strand at 151.586 kb on + strand at 151.586 kb on + strand at 151.587 kb on - strand at 151.714 kb on + strand, within Ac3H11_869 at 151.714 kb on + strand, within Ac3H11_869 at 151.715 kb on - strand, within Ac3H11_869 at 151.715 kb on - strand, within Ac3H11_869 at 151.715 kb on - strand, within Ac3H11_869 at 151.739 kb on - strand, within Ac3H11_869 at 151.739 kb on - strand, within Ac3H11_869 at 151.755 kb on + strand, within Ac3H11_869 at 151.755 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.798 kb on + strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 151.799 kb on - strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.284 kb on + strand, within Ac3H11_869 at 152.285 kb on - strand, within Ac3H11_869 at 152.285 kb on - strand, within Ac3H11_869 at 152.285 kb on - strand, within Ac3H11_869 at 152.611 kb on + strand at 152.612 kb on - strand at 152.612 kb on - strand at 152.670 kb on - strand at 152.682 kb on - strand at 152.742 kb on - strand, within Ac3H11_870 at 152.978 kb on + strand, within Ac3H11_870 at 152.978 kb on + strand, within Ac3H11_870 at 152.978 kb on + strand, within Ac3H11_870 at 152.979 kb on - strand at 152.979 kb on - strand at 152.996 kb on + strand at 152.996 kb on + strand at 152.996 kb on + strand at 152.996 kb on + strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 152.997 kb on - strand at 153.027 kb on - strand at 153.243 kb on + strand, within Ac3H11_872 at 153.243 kb on + strand, within Ac3H11_872 at 153.243 kb on + strand, within Ac3H11_872 at 153.243 kb on + strand, within Ac3H11_872 at 153.243 kb on + strand, within Ac3H11_872 at 153.243 kb on + strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.244 kb on - strand, within Ac3H11_872 at 153.330 kb on + strand, within Ac3H11_872 at 153.330 kb on + strand, within Ac3H11_872 at 153.330 kb on + strand, within Ac3H11_872 at 153.330 kb on + strand, within Ac3H11_872 at 153.330 kb on + strand, within Ac3H11_872 at 153.331 kb on - strand, within Ac3H11_872 at 153.331 kb on - strand, within Ac3H11_872 at 153.331 kb on - strand, within Ac3H11_872 at 153.331 kb on - strand, within Ac3H11_872 at 153.434 kb on + strand at 153.434 kb on + strand at 153.435 kb on - strand at 153.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 150,594 - Ac3H11_868 0.36 +0.5 150,594 - Ac3H11_868 0.36 -0.7 150,594 - Ac3H11_868 0.36 -0.8 150,594 - Ac3H11_868 0.36 -1.4 150,594 - Ac3H11_868 0.36 -0.8 150,594 - Ac3H11_868 0.36 -0.9 150,594 - Ac3H11_868 0.36 -1.7 150,594 - Ac3H11_868 0.36 +0.0 150,594 - Ac3H11_868 0.36 +0.5 150,594 - Ac3H11_868 0.36 +0.1 150,594 - Ac3H11_868 0.36 -0.4 150,701 + Ac3H11_868 0.45 -0.5 150,716 + Ac3H11_868 0.46 -0.4 150,717 - Ac3H11_868 0.46 +0.3 150,845 + Ac3H11_868 0.56 +0.9 150,845 + Ac3H11_868 0.56 -0.3 150,845 + Ac3H11_868 0.56 +1.0 150,845 + Ac3H11_868 0.56 -0.2 150,845 + Ac3H11_868 0.56 -1.4 150,846 - Ac3H11_868 0.57 -0.8 150,846 - Ac3H11_868 0.57 -1.2 150,846 - Ac3H11_868 0.57 +0.6 150,846 - Ac3H11_868 0.57 -1.3 150,846 - Ac3H11_868 0.57 +0.0 150,846 - Ac3H11_868 0.57 -0.8 150,846 - Ac3H11_868 0.57 -2.0 150,851 + Ac3H11_868 0.57 -0.5 150,851 + Ac3H11_868 0.57 -1.4 150,852 - Ac3H11_868 0.57 -1.2 150,852 - Ac3H11_868 0.57 -0.5 150,852 - Ac3H11_868 0.57 -0.6 150,852 - Ac3H11_868 0.57 +0.6 150,852 - Ac3H11_868 0.57 -0.5 150,852 - Ac3H11_868 0.57 -0.3 150,852 - Ac3H11_868 0.57 +0.3 151,049 + Ac3H11_868 0.73 -1.5 151,050 - Ac3H11_868 0.74 +0.3 151,050 - Ac3H11_868 0.74 -0.1 151,115 + Ac3H11_868 0.79 +0.2 151,115 + Ac3H11_868 0.79 +0.8 151,115 + Ac3H11_868 0.79 -1.8 151,115 + Ac3H11_868 0.79 +1.2 151,115 + Ac3H11_868 0.79 +0.7 151,115 + Ac3H11_868 0.79 -1.2 151,115 + Ac3H11_868 0.79 -1.2 151,115 + Ac3H11_868 0.79 -2.5 151,116 - Ac3H11_868 0.79 +2.6 151,116 - Ac3H11_868 0.79 -1.5 151,116 - Ac3H11_868 0.79 +0.8 151,127 + Ac3H11_868 0.80 -1.2 151,127 + Ac3H11_868 0.80 -0.3 151,127 + Ac3H11_868 0.80 -2.0 151,127 + Ac3H11_868 0.80 -0.6 151,127 + Ac3H11_868 0.80 +0.5 151,128 - Ac3H11_868 0.80 +1.3 151,128 - Ac3H11_868 0.80 +0.3 151,128 - Ac3H11_868 0.80 -1.5 151,128 - Ac3H11_868 0.80 -2.9 151,128 - Ac3H11_868 0.80 -1.9 151,128 - Ac3H11_868 0.80 -1.3 151,205 + Ac3H11_868 0.86 -0.8 151,205 + Ac3H11_868 0.86 -2.6 151,205 + Ac3H11_868 0.86 +2.4 151,206 - Ac3H11_868 0.87 +0.5 151,206 - Ac3H11_868 0.87 -1.3 151,206 - Ac3H11_868 0.87 -0.1 151,208 + Ac3H11_868 0.87 +0.5 151,208 + Ac3H11_868 0.87 -1.1 151,208 + Ac3H11_868 0.87 -2.4 151,208 + Ac3H11_868 0.87 +0.7 151,208 + Ac3H11_868 0.87 +1.5 151,208 + Ac3H11_868 0.87 -0.3 151,209 - Ac3H11_868 0.87 +0.3 151,209 - Ac3H11_868 0.87 -0.4 151,209 - Ac3H11_868 0.87 +0.6 151,209 - Ac3H11_868 0.87 -0.1 151,209 - Ac3H11_868 0.87 -0.0 151,226 + Ac3H11_868 0.88 +0.1 151,226 + Ac3H11_868 0.88 -0.3 151,226 + Ac3H11_868 0.88 -1.5 151,226 + Ac3H11_868 0.88 +0.0 151,227 - Ac3H11_868 0.88 -0.7 151,227 - Ac3H11_868 0.88 -0.4 151,319 + -0.4 151,418 - +1.0 151,418 - +0.3 151,426 + +0.8 151,426 + -1.0 151,427 - +0.0 151,427 - -0.3 151,427 - +0.3 151,452 + -0.7 151,452 + -0.4 151,452 + -2.7 151,452 + +0.3 151,452 + +0.8 151,453 - -0.4 151,453 - -0.1 151,453 - -0.1 151,453 - +0.3 151,453 - -3.0 151,456 + +0.9 151,456 + +6.6 151,456 + +0.6 151,499 + -0.1 151,586 + -1.7 151,586 + -1.5 151,586 + -1.0 151,586 + +1.3 151,586 + -0.7 151,586 + -1.0 151,587 - +0.3 151,714 + Ac3H11_869 0.13 -0.2 151,714 + Ac3H11_869 0.13 +0.3 151,715 - Ac3H11_869 0.13 +1.6 151,715 - Ac3H11_869 0.13 -0.4 151,715 - Ac3H11_869 0.13 -0.3 151,739 - Ac3H11_869 0.15 -2.1 151,739 - Ac3H11_869 0.15 +0.2 151,755 + Ac3H11_869 0.17 +0.2 151,755 + Ac3H11_869 0.17 +2.9 151,798 + Ac3H11_869 0.21 -0.8 151,798 + Ac3H11_869 0.21 -1.0 151,798 + Ac3H11_869 0.21 -1.1 151,798 + Ac3H11_869 0.21 -2.5 151,798 + Ac3H11_869 0.21 -0.4 151,798 + Ac3H11_869 0.21 +0.9 151,798 + Ac3H11_869 0.21 -0.8 151,798 + Ac3H11_869 0.21 +0.5 151,798 + Ac3H11_869 0.21 -0.3 151,798 + Ac3H11_869 0.21 -2.3 151,798 + Ac3H11_869 0.21 +0.0 151,798 + Ac3H11_869 0.21 -1.9 151,798 + Ac3H11_869 0.21 -0.2 151,798 + Ac3H11_869 0.21 -1.4 151,798 + Ac3H11_869 0.21 -2.5 151,798 + Ac3H11_869 0.21 +0.9 151,798 + Ac3H11_869 0.21 -1.2 151,798 + Ac3H11_869 0.21 +0.8 151,798 + Ac3H11_869 0.21 -1.4 151,798 + Ac3H11_869 0.21 +0.2 151,798 + Ac3H11_869 0.21 -0.5 151,798 + Ac3H11_869 0.21 -1.0 151,798 + Ac3H11_869 0.21 +0.6 151,798 + Ac3H11_869 0.21 +4.0 151,799 - Ac3H11_869 0.21 +2.2 151,799 - Ac3H11_869 0.21 -0.4 151,799 - Ac3H11_869 0.21 -1.6 151,799 - Ac3H11_869 0.21 -1.9 151,799 - Ac3H11_869 0.21 +0.5 151,799 - Ac3H11_869 0.21 -1.2 151,799 - Ac3H11_869 0.21 +0.5 151,799 - Ac3H11_869 0.21 +0.2 151,799 - Ac3H11_869 0.21 -0.5 151,799 - Ac3H11_869 0.21 -1.5 151,799 - Ac3H11_869 0.21 +0.3 151,799 - Ac3H11_869 0.21 +1.1 151,799 - Ac3H11_869 0.21 -1.5 151,799 - Ac3H11_869 0.21 -0.4 151,799 - Ac3H11_869 0.21 +1.8 151,799 - Ac3H11_869 0.21 +0.9 151,799 - Ac3H11_869 0.21 -0.7 151,799 - Ac3H11_869 0.21 +0.3 151,799 - Ac3H11_869 0.21 -0.7 151,799 - Ac3H11_869 0.21 -1.5 151,799 - Ac3H11_869 0.21 +1.0 151,799 - Ac3H11_869 0.21 -1.1 151,799 - Ac3H11_869 0.21 +1.0 151,799 - Ac3H11_869 0.21 +0.0 151,799 - Ac3H11_869 0.21 -0.5 151,799 - Ac3H11_869 0.21 +0.4 151,799 - Ac3H11_869 0.21 -1.5 151,799 - Ac3H11_869 0.21 +1.2 151,799 - Ac3H11_869 0.21 +0.6 151,799 - Ac3H11_869 0.21 +0.0 151,799 - Ac3H11_869 0.21 -1.5 151,799 - Ac3H11_869 0.21 -0.6 152,284 + Ac3H11_869 0.72 -1.1 152,284 + Ac3H11_869 0.72 -1.6 152,284 + Ac3H11_869 0.72 -1.2 152,284 + Ac3H11_869 0.72 +2.3 152,284 + Ac3H11_869 0.72 +1.1 152,284 + Ac3H11_869 0.72 -1.3 152,285 - Ac3H11_869 0.72 +3.5 152,285 - Ac3H11_869 0.72 -0.0 152,285 - Ac3H11_869 0.72 -0.1 152,611 + -0.5 152,612 - +0.1 152,612 - -0.0 152,670 - +1.9 152,682 - +1.6 152,742 - Ac3H11_870 0.19 -0.9 152,978 + Ac3H11_870 0.90 -0.1 152,978 + Ac3H11_870 0.90 +0.2 152,978 + Ac3H11_870 0.90 -0.3 152,979 - -0.8 152,979 - -0.1 152,996 + -1.5 152,996 + +1.6 152,996 + -0.5 152,996 + -0.2 152,997 - -0.2 152,997 - -0.4 152,997 - +1.5 152,997 - +0.4 152,997 - +1.6 152,997 - -0.9 152,997 - +0.0 152,997 - -1.0 152,997 - -1.0 152,997 - -0.2 152,997 - +0.8 152,997 - -0.2 153,027 - -0.9 153,243 + Ac3H11_872 0.47 -2.8 153,243 + Ac3H11_872 0.47 +1.9 153,243 + Ac3H11_872 0.47 +0.2 153,243 + Ac3H11_872 0.47 -0.3 153,243 + Ac3H11_872 0.47 -1.8 153,243 + Ac3H11_872 0.47 -1.6 153,244 - Ac3H11_872 0.47 +0.9 153,244 - Ac3H11_872 0.47 +1.6 153,244 - Ac3H11_872 0.47 +0.6 153,244 - Ac3H11_872 0.47 +1.5 153,244 - Ac3H11_872 0.47 -0.3 153,244 - Ac3H11_872 0.47 -0.4 153,330 + Ac3H11_872 0.83 +3.0 153,330 + Ac3H11_872 0.83 +1.3 153,330 + Ac3H11_872 0.83 -2.4 153,330 + Ac3H11_872 0.83 +1.4 153,330 + Ac3H11_872 0.83 -2.3 153,331 - Ac3H11_872 0.83 -0.4 153,331 - Ac3H11_872 0.83 +1.4 153,331 - Ac3H11_872 0.83 -2.1 153,331 - Ac3H11_872 0.83 -0.8 153,434 + +0.2 153,434 + -0.6 153,435 - -1.0 153,435 - -0.1
Or see this region's nucleotide sequence