Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_73 and Ac3H11_74 are separated by 27 nucleotides Ac3H11_74 and Ac3H11_75 are separated by 111 nucleotides Ac3H11_75 and Ac3H11_76 are separated by 8 nucleotides Ac3H11_76 and Ac3H11_77 are separated by 24 nucleotides Ac3H11_77 and Ac3H11_78 overlap by 4 nucleotides
Ac3H11_73 - Phenylalanyl-tRNA synthetase beta subunit, at 77,407 to 77,874
_73
Ac3H11_74 - hypothetical protein, at 77,902 to 78,144
_74
Ac3H11_75 - Outer membrane lipoprotein blc precursor, at 78,256 to 78,822
_75
Ac3H11_76 - COG3380: Amine oxidase, flavin-containing, at 78,831 to 79,835
_76
Ac3H11_77 - Gsl3115 protein, at 79,860 to 80,036
_77
Ac3H11_78 - Cryptochrome, at 80,033 to 81,349
_78
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 77.866 kb on + strand at 77.866 kb on + strand at 77.867 kb on - strand at 77.867 kb on - strand at 77.872 kb on + strand at 77.873 kb on - strand at 78.012 kb on - strand, within Ac3H11_74 at 78.012 kb on - strand, within Ac3H11_74 at 78.123 kb on - strand at 78.155 kb on + strand at 78.255 kb on + strand at 78.466 kb on + strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.613 kb on + strand, within Ac3H11_75 at 78.622 kb on + strand, within Ac3H11_75 at 78.649 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.830 kb on + strand at 78.830 kb on + strand at 78.830 kb on + strand at 78.831 kb on - strand at 78.831 kb on - strand at 79.014 kb on + strand, within Ac3H11_76 at 79.684 kb on - strand, within Ac3H11_76 at 79.833 kb on + strand at 79.834 kb on - strand at 79.860 kb on - strand at 79.959 kb on + strand, within Ac3H11_77 at 79.960 kb on - strand, within Ac3H11_77 at 80.358 kb on - strand, within Ac3H11_78 at 80.408 kb on - strand, within Ac3H11_78 at 80.726 kb on + strand, within Ac3H11_78 at 80.727 kb on - strand, within Ac3H11_78 at 80.754 kb on - strand, within Ac3H11_78 at 80.789 kb on + strand, within Ac3H11_78 at 80.790 kb on - strand, within Ac3H11_78
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 77,866 + +0.0 77,866 + +0.2 77,867 - -0.1 77,867 - -0.0 77,872 + +1.5 77,873 - +1.1 78,012 - Ac3H11_74 0.45 -1.6 78,012 - Ac3H11_74 0.45 +0.4 78,123 - -2.0 78,155 + -2.2 78,255 + -0.0 78,466 + Ac3H11_75 0.37 -2.6 78,467 - Ac3H11_75 0.37 +1.2 78,467 - Ac3H11_75 0.37 -0.7 78,613 + Ac3H11_75 0.63 -1.1 78,622 + Ac3H11_75 0.65 -1.5 78,649 + Ac3H11_75 0.69 -1.3 78,685 + Ac3H11_75 0.76 +2.2 78,685 + Ac3H11_75 0.76 -0.3 78,685 + Ac3H11_75 0.76 +1.9 78,686 - Ac3H11_75 0.76 +0.7 78,686 - Ac3H11_75 0.76 +1.6 78,830 + +0.2 78,830 + +1.1 78,830 + -0.8 78,831 - -0.9 78,831 - +0.0 79,014 + Ac3H11_76 0.18 +2.8 79,684 - Ac3H11_76 0.85 -0.3 79,833 + +0.9 79,834 - -0.6 79,860 - +1.4 79,959 + Ac3H11_77 0.56 -0.2 79,960 - Ac3H11_77 0.56 +0.5 80,358 - Ac3H11_78 0.25 -1.8 80,408 - Ac3H11_78 0.28 -1.3 80,726 + Ac3H11_78 0.53 +0.1 80,727 - Ac3H11_78 0.53 -0.1 80,754 - Ac3H11_78 0.55 -0.3 80,789 + Ac3H11_78 0.57 -0.3 80,790 - Ac3H11_78 0.57 -0.3
Or see this region's nucleotide sequence