Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_475 and Ac3H11_476 are separated by 222 nucleotides Ac3H11_476 and Ac3H11_477 are separated by 48 nucleotides Ac3H11_477 and Ac3H11_478 overlap by 4 nucleotides Ac3H11_478 and Ac3H11_479 overlap by 2 nucleotides
Ac3H11_475 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 171,157 to 172,233
_475
Ac3H11_476 - hypothetical protein, at 172,456 to 172,608
_476
Ac3H11_477 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 172,657 to 174,171
_477
Ac3H11_478 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 174,168 to 174,953
_478
Ac3H11_479 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 174,952 to 175,809
_479
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 171.861 kb on - strand, within Ac3H11_475 at 171.893 kb on - strand, within Ac3H11_475 at 171.893 kb on - strand, within Ac3H11_475 at 171.945 kb on + strand, within Ac3H11_475 at 172.021 kb on + strand, within Ac3H11_475 at 172.022 kb on - strand, within Ac3H11_475 at 172.022 kb on - strand, within Ac3H11_475 at 172.022 kb on - strand, within Ac3H11_475 at 172.045 kb on + strand, within Ac3H11_475 at 172.045 kb on + strand, within Ac3H11_475 at 172.045 kb on + strand, within Ac3H11_475 at 172.046 kb on - strand, within Ac3H11_475 at 172.046 kb on - strand, within Ac3H11_475 at 172.046 kb on - strand, within Ac3H11_475 at 172.186 kb on + strand at 172.186 kb on + strand at 172.187 kb on - strand at 172.187 kb on - strand at 172.187 kb on - strand at 172.222 kb on + strand at 172.222 kb on + strand at 172.222 kb on + strand at 172.222 kb on + strand at 172.222 kb on + strand at 172.223 kb on - strand at 172.223 kb on - strand at 172.327 kb on + strand at 172.327 kb on + strand at 172.327 kb on + strand at 172.328 kb on - strand at 172.328 kb on - strand at 172.328 kb on - strand at 172.328 kb on - strand at 172.328 kb on - strand at 172.552 kb on + strand, within Ac3H11_476 at 172.552 kb on + strand, within Ac3H11_476 at 172.552 kb on + strand, within Ac3H11_476 at 172.552 kb on + strand, within Ac3H11_476 at 172.552 kb on + strand, within Ac3H11_476 at 172.553 kb on - strand, within Ac3H11_476 at 172.553 kb on - strand, within Ac3H11_476 at 172.553 kb on - strand, within Ac3H11_476 at 172.553 kb on - strand, within Ac3H11_476 at 172.574 kb on + strand, within Ac3H11_476 at 172.574 kb on + strand, within Ac3H11_476 at 172.575 kb on - strand, within Ac3H11_476 at 172.575 kb on - strand, within Ac3H11_476 at 172.772 kb on + strand at 172.772 kb on + strand at 172.772 kb on + strand at 172.773 kb on - strand at 172.773 kb on - strand at 172.857 kb on - strand, within Ac3H11_477 at 173.144 kb on + strand, within Ac3H11_477 at 173.145 kb on - strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.167 kb on + strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.168 kb on - strand, within Ac3H11_477 at 173.250 kb on - strand, within Ac3H11_477 at 173.250 kb on - strand, within Ac3H11_477 at 173.250 kb on - strand, within Ac3H11_477 at 173.348 kb on + strand, within Ac3H11_477 at 173.348 kb on + strand, within Ac3H11_477 at 173.349 kb on - strand, within Ac3H11_477 at 173.413 kb on + strand, within Ac3H11_477 at 173.413 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.435 kb on + strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.436 kb on - strand, within Ac3H11_477 at 173.471 kb on + strand, within Ac3H11_477 at 173.472 kb on - strand, within Ac3H11_477 at 173.541 kb on - strand, within Ac3H11_477 at 173.541 kb on - strand, within Ac3H11_477 at 173.541 kb on - strand, within Ac3H11_477 at 173.541 kb on - strand, within Ac3H11_477 at 173.594 kb on + strand, within Ac3H11_477 at 173.623 kb on + strand, within Ac3H11_477 at 173.623 kb on + strand, within Ac3H11_477 at 173.623 kb on + strand, within Ac3H11_477 at 173.623 kb on + strand, within Ac3H11_477 at 173.669 kb on + strand, within Ac3H11_477 at 173.669 kb on + strand, within Ac3H11_477 at 173.669 kb on + strand, within Ac3H11_477 at 173.670 kb on - strand, within Ac3H11_477 at 173.670 kb on - strand, within Ac3H11_477 at 173.670 kb on - strand, within Ac3H11_477 at 173.753 kb on + strand, within Ac3H11_477 at 173.754 kb on - strand, within Ac3H11_477 at 173.754 kb on - strand, within Ac3H11_477 at 173.819 kb on + strand, within Ac3H11_477 at 173.819 kb on + strand, within Ac3H11_477 at 173.852 kb on + strand, within Ac3H11_477 at 173.852 kb on + strand, within Ac3H11_477 at 173.853 kb on - strand, within Ac3H11_477 at 173.853 kb on - strand, within Ac3H11_477 at 173.870 kb on + strand, within Ac3H11_477 at 173.871 kb on - strand, within Ac3H11_477 at 173.871 kb on - strand, within Ac3H11_477 at 174.036 kb on + strand at 174.036 kb on + strand at 174.037 kb on - strand at 174.037 kb on - strand at 174.037 kb on - strand at 174.037 kb on - strand at 174.373 kb on + strand, within Ac3H11_478 at 174.374 kb on - strand, within Ac3H11_478 at 174.436 kb on + strand, within Ac3H11_478 at 174.436 kb on + strand, within Ac3H11_478 at 174.437 kb on - strand, within Ac3H11_478 at 174.437 kb on - strand, within Ac3H11_478 at 174.567 kb on + strand, within Ac3H11_478 at 174.567 kb on + strand, within Ac3H11_478 at 174.568 kb on - strand, within Ac3H11_478 at 174.568 kb on - strand, within Ac3H11_478 at 174.568 kb on - strand, within Ac3H11_478 at 174.568 kb on - strand, within Ac3H11_478 at 174.598 kb on + strand, within Ac3H11_478 at 174.598 kb on + strand, within Ac3H11_478 at 174.598 kb on + strand, within Ac3H11_478 at 174.598 kb on + strand, within Ac3H11_478 at 174.598 kb on + strand, within Ac3H11_478 at 174.599 kb on - strand, within Ac3H11_478 at 174.599 kb on - strand, within Ac3H11_478 at 174.599 kb on - strand, within Ac3H11_478 at 174.601 kb on + strand, within Ac3H11_478 at 174.601 kb on + strand, within Ac3H11_478 at 174.601 kb on + strand, within Ac3H11_478 at 174.601 kb on + strand, within Ac3H11_478 at 174.602 kb on - strand, within Ac3H11_478 at 174.602 kb on - strand, within Ac3H11_478 at 174.737 kb on - strand, within Ac3H11_478 at 174.767 kb on + strand, within Ac3H11_478 at 174.768 kb on - strand, within Ac3H11_478 at 174.768 kb on - strand, within Ac3H11_478 at 174.807 kb on + strand, within Ac3H11_478 at 174.807 kb on + strand, within Ac3H11_478 at 174.807 kb on + strand, within Ac3H11_478 at 174.807 kb on + strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.808 kb on - strand, within Ac3H11_478 at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.916 kb on + strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.917 kb on - strand at 174.966 kb on + strand at 174.966 kb on + strand at 174.966 kb on + strand at 174.966 kb on + strand at 174.967 kb on - strand at 174.967 kb on - strand at 174.967 kb on - strand at 174.968 kb on + strand at 174.969 kb on - strand at 175.007 kb on - strand at 175.063 kb on + strand, within Ac3H11_479 at 175.064 kb on - strand, within Ac3H11_479 at 175.123 kb on + strand, within Ac3H11_479 at 175.124 kb on - strand, within Ac3H11_479
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 171,861 - Ac3H11_475 0.65 +0.7 171,893 - Ac3H11_475 0.68 +0.8 171,893 - Ac3H11_475 0.68 +0.5 171,945 + Ac3H11_475 0.73 +0.4 172,021 + Ac3H11_475 0.80 -1.0 172,022 - Ac3H11_475 0.80 -0.6 172,022 - Ac3H11_475 0.80 +1.5 172,022 - Ac3H11_475 0.80 +0.9 172,045 + Ac3H11_475 0.82 -0.5 172,045 + Ac3H11_475 0.82 +1.7 172,045 + Ac3H11_475 0.82 +0.2 172,046 - Ac3H11_475 0.83 +0.8 172,046 - Ac3H11_475 0.83 +2.9 172,046 - Ac3H11_475 0.83 +0.8 172,186 + -2.2 172,186 + +0.9 172,187 - -0.5 172,187 - -0.5 172,187 - +1.2 172,222 + +1.0 172,222 + -1.3 172,222 + -1.7 172,222 + +1.1 172,222 + -0.3 172,223 - +2.1 172,223 - -0.9 172,327 + -1.7 172,327 + -0.1 172,327 + +0.2 172,328 - +2.1 172,328 - -1.4 172,328 - -0.2 172,328 - -1.3 172,328 - -0.1 172,552 + Ac3H11_476 0.63 -0.4 172,552 + Ac3H11_476 0.63 -0.4 172,552 + Ac3H11_476 0.63 -0.7 172,552 + Ac3H11_476 0.63 -0.7 172,552 + Ac3H11_476 0.63 -0.8 172,553 - Ac3H11_476 0.63 -0.8 172,553 - Ac3H11_476 0.63 +1.6 172,553 - Ac3H11_476 0.63 +0.1 172,553 - Ac3H11_476 0.63 -1.6 172,574 + Ac3H11_476 0.77 +0.2 172,574 + Ac3H11_476 0.77 -0.3 172,575 - Ac3H11_476 0.78 -1.1 172,575 - Ac3H11_476 0.78 +0.0 172,772 + -2.1 172,772 + +1.1 172,772 + -1.6 172,773 - -0.1 172,773 - -1.7 172,857 - Ac3H11_477 0.13 +0.5 173,144 + Ac3H11_477 0.32 +0.7 173,145 - Ac3H11_477 0.32 +3.4 173,167 + Ac3H11_477 0.34 -0.7 173,167 + Ac3H11_477 0.34 -0.6 173,167 + Ac3H11_477 0.34 -1.5 173,167 + Ac3H11_477 0.34 +0.8 173,167 + Ac3H11_477 0.34 -0.6 173,167 + Ac3H11_477 0.34 +1.2 173,167 + Ac3H11_477 0.34 -1.7 173,167 + Ac3H11_477 0.34 -0.3 173,168 - Ac3H11_477 0.34 +0.6 173,168 - Ac3H11_477 0.34 -1.4 173,168 - Ac3H11_477 0.34 -1.7 173,168 - Ac3H11_477 0.34 -1.3 173,168 - Ac3H11_477 0.34 +0.2 173,168 - Ac3H11_477 0.34 -0.3 173,250 - Ac3H11_477 0.39 -0.7 173,250 - Ac3H11_477 0.39 -0.3 173,250 - Ac3H11_477 0.39 +1.9 173,348 + Ac3H11_477 0.46 -1.4 173,348 + Ac3H11_477 0.46 +2.5 173,349 - Ac3H11_477 0.46 -1.5 173,413 + Ac3H11_477 0.50 -1.6 173,413 + Ac3H11_477 0.50 +0.9 173,435 + Ac3H11_477 0.51 +0.3 173,435 + Ac3H11_477 0.51 -1.0 173,435 + Ac3H11_477 0.51 -0.4 173,435 + Ac3H11_477 0.51 +0.5 173,435 + Ac3H11_477 0.51 -0.7 173,435 + Ac3H11_477 0.51 +2.8 173,435 + Ac3H11_477 0.51 +0.5 173,435 + Ac3H11_477 0.51 -1.3 173,435 + Ac3H11_477 0.51 -2.4 173,435 + Ac3H11_477 0.51 +1.6 173,435 + Ac3H11_477 0.51 -0.5 173,435 + Ac3H11_477 0.51 -0.8 173,435 + Ac3H11_477 0.51 -0.8 173,435 + Ac3H11_477 0.51 -1.5 173,435 + Ac3H11_477 0.51 -0.1 173,435 + Ac3H11_477 0.51 -2.3 173,435 + +2.5 173,435 + Ac3H11_477 0.51 +0.5 173,435 + Ac3H11_477 0.51 +0.1 173,435 + Ac3H11_477 0.51 +0.2 173,435 + Ac3H11_477 0.51 -0.7 173,435 + Ac3H11_477 0.51 -0.4 173,435 + Ac3H11_477 0.51 -2.5 173,435 + Ac3H11_477 0.51 +0.2 173,436 - Ac3H11_477 0.51 -1.2 173,436 - Ac3H11_477 0.51 +0.5 173,436 - Ac3H11_477 0.51 -1.6 173,436 - Ac3H11_477 0.51 +0.1 173,436 - Ac3H11_477 0.51 -0.2 173,436 - Ac3H11_477 0.51 -1.0 173,436 - Ac3H11_477 0.51 +0.2 173,436 - Ac3H11_477 0.51 -2.8 173,436 - Ac3H11_477 0.51 +0.1 173,436 - Ac3H11_477 0.51 -1.7 173,436 - Ac3H11_477 0.51 -0.6 173,436 - Ac3H11_477 0.51 +0.7 173,436 - Ac3H11_477 0.51 -0.0 173,436 - Ac3H11_477 0.51 -0.7 173,436 - Ac3H11_477 0.51 -1.2 173,436 - Ac3H11_477 0.51 +0.2 173,436 - Ac3H11_477 0.51 -1.0 173,436 - Ac3H11_477 0.51 +1.0 173,471 + Ac3H11_477 0.54 +0.3 173,472 - Ac3H11_477 0.54 -1.2 173,541 - Ac3H11_477 0.58 -0.1 173,541 - Ac3H11_477 0.58 +0.5 173,541 - Ac3H11_477 0.58 -1.2 173,541 - Ac3H11_477 0.58 -0.7 173,594 + Ac3H11_477 0.62 -0.8 173,623 + Ac3H11_477 0.64 -0.7 173,623 + Ac3H11_477 0.64 -1.2 173,623 + Ac3H11_477 0.64 -0.2 173,623 + Ac3H11_477 0.64 -0.3 173,669 + Ac3H11_477 0.67 -1.3 173,669 + Ac3H11_477 0.67 -0.2 173,669 + Ac3H11_477 0.67 -2.1 173,670 - Ac3H11_477 0.67 +1.2 173,670 - Ac3H11_477 0.67 -0.8 173,670 - Ac3H11_477 0.67 -0.9 173,753 + Ac3H11_477 0.72 +0.5 173,754 - Ac3H11_477 0.72 -0.3 173,754 - Ac3H11_477 0.72 -1.5 173,819 + Ac3H11_477 0.77 -2.0 173,819 + Ac3H11_477 0.77 +1.5 173,852 + Ac3H11_477 0.79 -1.9 173,852 + Ac3H11_477 0.79 +1.3 173,853 - Ac3H11_477 0.79 -2.7 173,853 - Ac3H11_477 0.79 -1.2 173,870 + Ac3H11_477 0.80 -0.4 173,871 - Ac3H11_477 0.80 -0.5 173,871 - Ac3H11_477 0.80 +0.4 174,036 + +0.6 174,036 + -0.6 174,037 - +0.6 174,037 - -1.3 174,037 - -0.2 174,037 - -0.7 174,373 + Ac3H11_478 0.26 +0.1 174,374 - Ac3H11_478 0.26 -0.5 174,436 + Ac3H11_478 0.34 -1.7 174,436 + Ac3H11_478 0.34 -0.7 174,437 - Ac3H11_478 0.34 -1.3 174,437 - Ac3H11_478 0.34 -0.3 174,567 + Ac3H11_478 0.51 -1.9 174,567 + Ac3H11_478 0.51 -2.1 174,568 - Ac3H11_478 0.51 +0.8 174,568 - Ac3H11_478 0.51 +0.5 174,568 - Ac3H11_478 0.51 +1.3 174,568 - Ac3H11_478 0.51 +1.8 174,598 + Ac3H11_478 0.55 +0.8 174,598 + Ac3H11_478 0.55 -0.0 174,598 + Ac3H11_478 0.55 -1.7 174,598 + Ac3H11_478 0.55 -0.4 174,598 + Ac3H11_478 0.55 -0.6 174,599 - Ac3H11_478 0.55 -1.3 174,599 - Ac3H11_478 0.55 -1.4 174,599 - Ac3H11_478 0.55 +1.1 174,601 + Ac3H11_478 0.55 -0.5 174,601 + Ac3H11_478 0.55 -0.5 174,601 + Ac3H11_478 0.55 +0.8 174,601 + Ac3H11_478 0.55 -1.9 174,602 - Ac3H11_478 0.55 -0.1 174,602 - Ac3H11_478 0.55 -0.9 174,737 - Ac3H11_478 0.72 -2.1 174,767 + Ac3H11_478 0.76 +2.5 174,768 - Ac3H11_478 0.76 +1.8 174,768 - Ac3H11_478 0.76 +0.5 174,807 + Ac3H11_478 0.81 -0.8 174,807 + Ac3H11_478 0.81 -0.9 174,807 + Ac3H11_478 0.81 -2.3 174,807 + Ac3H11_478 0.81 -0.1 174,808 - Ac3H11_478 0.81 -0.9 174,808 - Ac3H11_478 0.81 +0.0 174,808 - Ac3H11_478 0.81 -2.1 174,808 - Ac3H11_478 0.81 +0.0 174,808 - Ac3H11_478 0.81 +0.1 174,808 - Ac3H11_478 0.81 -1.3 174,916 + -1.2 174,916 + -2.8 174,916 + -0.7 174,916 + +0.7 174,916 + -0.8 174,916 + -1.0 174,916 + +0.3 174,916 + +0.6 174,916 + -1.7 174,917 - -0.1 174,917 - +3.0 174,917 - +4.4 174,917 - +3.0 174,917 - +3.5 174,917 - +0.1 174,917 - -0.4 174,917 - +0.5 174,917 - -0.2 174,917 - +2.6 174,966 + +1.9 174,966 + +0.3 174,966 + +0.8 174,966 + +0.2 174,967 - +0.7 174,967 - +4.0 174,967 - +5.3 174,968 + -1.4 174,969 - +1.0 175,007 - +5.8 175,063 + Ac3H11_479 0.13 +1.1 175,064 - Ac3H11_479 0.13 +3.9 175,123 + Ac3H11_479 0.20 +2.8 175,124 - Ac3H11_479 0.20 +5.1
Or see this region's nucleotide sequence