Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4711 and Ac3H11_4712 are separated by 276 nucleotides Ac3H11_4712 and Ac3H11_4713 overlap by 4 nucleotides Ac3H11_4713 and Ac3H11_4714 are separated by 36 nucleotides
Ac3H11_4711 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 22,191 to 23,669
_4711
Ac3H11_4712 - Methionine ABC transporter ATP-binding protein, at 23,946 to 24,809
_4712
Ac3H11_4713 - Uncharacterized ABC transporter, permease component YrbE, at 24,806 to 25,588
_4713
Ac3H11_4714 - Uncharacterized ABC transporter, periplasmic component YrbD, at 25,625 to 26,113
_4714
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 23.226 kb on + strand, within Ac3H11_4711 at 23.226 kb on + strand, within Ac3H11_4711 at 23.400 kb on + strand, within Ac3H11_4711 at 23.400 kb on + strand, within Ac3H11_4711 at 23.400 kb on + strand, within Ac3H11_4711 at 23.400 kb on + strand, within Ac3H11_4711 at 23.401 kb on - strand, within Ac3H11_4711 at 23.401 kb on - strand, within Ac3H11_4711 at 23.527 kb on - strand at 23.650 kb on - strand at 23.650 kb on - strand at 23.760 kb on + strand at 23.760 kb on + strand at 23.892 kb on + strand at 23.892 kb on + strand at 23.892 kb on + strand at 23.892 kb on + strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.893 kb on - strand at 24.039 kb on + strand, within Ac3H11_4712 at 24.040 kb on - strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.228 kb on + strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.229 kb on - strand, within Ac3H11_4712 at 24.422 kb on - strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.489 kb on + strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.490 kb on - strand, within Ac3H11_4712 at 24.754 kb on - strand at 24.815 kb on + strand at 24.815 kb on + strand at 24.815 kb on + strand at 24.816 kb on - strand at 24.816 kb on - strand at 24.816 kb on - strand at 24.965 kb on + strand, within Ac3H11_4713 at 24.965 kb on + strand, within Ac3H11_4713 at 24.965 kb on + strand, within Ac3H11_4713 at 24.965 kb on + strand, within Ac3H11_4713 at 24.965 kb on + strand, within Ac3H11_4713 at 24.965 kb on + strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 24.966 kb on - strand, within Ac3H11_4713 at 25.028 kb on + strand, within Ac3H11_4713 at 25.028 kb on + strand, within Ac3H11_4713 at 25.029 kb on - strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.031 kb on + strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.032 kb on - strand, within Ac3H11_4713 at 25.046 kb on + strand, within Ac3H11_4713 at 25.582 kb on + strand at 25.582 kb on + strand at 25.582 kb on + strand at 25.582 kb on + strand at 25.582 kb on + strand at 25.582 kb on + strand at 25.582 kb on + strand at 25.583 kb on - strand at 25.583 kb on - strand at 25.583 kb on - strand at 25.583 kb on - strand at 25.742 kb on + strand, within Ac3H11_4714 at 25.742 kb on + strand, within Ac3H11_4714
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 23,226 + Ac3H11_4711 0.70 +0.3 23,226 + Ac3H11_4711 0.70 +0.6 23,400 + Ac3H11_4711 0.82 +1.3 23,400 + Ac3H11_4711 0.82 -0.6 23,400 + Ac3H11_4711 0.82 -0.5 23,400 + Ac3H11_4711 0.82 -1.1 23,401 - Ac3H11_4711 0.82 +0.1 23,401 - Ac3H11_4711 0.82 -1.5 23,527 - -0.5 23,650 - -0.5 23,650 - +2.2 23,760 + +0.7 23,760 + +0.3 23,892 + +1.1 23,892 + +0.6 23,892 + +0.3 23,892 + -1.4 23,893 - +1.2 23,893 - +3.0 23,893 - +0.7 23,893 - +0.3 23,893 - +0.9 23,893 - +3.0 23,893 - +2.5 23,893 - +0.6 23,893 - +0.2 23,893 - +2.6 24,039 + Ac3H11_4712 0.11 -0.5 24,040 - Ac3H11_4712 0.11 +0.7 24,228 + Ac3H11_4712 0.33 -1.1 24,228 + Ac3H11_4712 0.33 +3.4 24,228 + Ac3H11_4712 0.33 +2.2 24,228 + Ac3H11_4712 0.33 -0.8 24,228 + Ac3H11_4712 0.33 +2.2 24,228 + Ac3H11_4712 0.33 +3.1 24,228 + Ac3H11_4712 0.33 +1.5 24,228 + Ac3H11_4712 0.33 +3.5 24,228 + Ac3H11_4712 0.33 +3.7 24,228 + Ac3H11_4712 0.33 -0.9 24,228 + Ac3H11_4712 0.33 +2.1 24,228 + Ac3H11_4712 0.33 +2.3 24,228 + Ac3H11_4712 0.33 +2.3 24,228 + Ac3H11_4712 0.33 +0.6 24,228 + Ac3H11_4712 0.33 +1.8 24,228 + Ac3H11_4712 0.33 +2.4 24,228 + Ac3H11_4712 0.33 +1.2 24,229 - Ac3H11_4712 0.33 +1.0 24,229 - Ac3H11_4712 0.33 +0.7 24,229 - Ac3H11_4712 0.33 +0.3 24,229 - Ac3H11_4712 0.33 +1.4 24,229 - Ac3H11_4712 0.33 +1.6 24,229 - Ac3H11_4712 0.33 +1.8 24,229 - Ac3H11_4712 0.33 +1.3 24,229 - Ac3H11_4712 0.33 +1.9 24,229 - Ac3H11_4712 0.33 +1.5 24,229 - Ac3H11_4712 0.33 -1.3 24,422 - Ac3H11_4712 0.55 +0.6 24,489 + Ac3H11_4712 0.63 +2.4 24,489 + Ac3H11_4712 0.63 +0.1 24,489 + Ac3H11_4712 0.63 +1.2 24,489 + Ac3H11_4712 0.63 -0.9 24,489 + Ac3H11_4712 0.63 +2.1 24,489 + Ac3H11_4712 0.63 -1.3 24,489 + Ac3H11_4712 0.63 +0.8 24,489 + Ac3H11_4712 0.63 +0.3 24,489 + Ac3H11_4712 0.63 -0.0 24,489 + Ac3H11_4712 0.63 +0.6 24,490 - Ac3H11_4712 0.63 +3.0 24,490 - Ac3H11_4712 0.63 +1.3 24,490 - Ac3H11_4712 0.63 -0.4 24,490 - Ac3H11_4712 0.63 +4.3 24,490 - Ac3H11_4712 0.63 +3.1 24,490 - Ac3H11_4712 0.63 +0.6 24,490 - Ac3H11_4712 0.63 +1.3 24,490 - Ac3H11_4712 0.63 +4.3 24,490 - Ac3H11_4712 0.63 +0.8 24,754 - +2.6 24,815 + +2.2 24,815 + +2.8 24,815 + -2.1 24,816 - +1.1 24,816 - +1.5 24,816 - -1.4 24,965 + Ac3H11_4713 0.20 +3.4 24,965 + Ac3H11_4713 0.20 +0.3 24,965 + Ac3H11_4713 0.20 -0.9 24,965 + Ac3H11_4713 0.20 +1.9 24,965 + Ac3H11_4713 0.20 +0.5 24,965 + Ac3H11_4713 0.20 +0.3 24,966 - Ac3H11_4713 0.20 +3.2 24,966 - Ac3H11_4713 0.20 +0.6 24,966 - Ac3H11_4713 0.20 +2.6 24,966 - Ac3H11_4713 0.20 +0.3 24,966 - Ac3H11_4713 0.20 +1.1 24,966 - Ac3H11_4713 0.20 +4.9 24,966 - Ac3H11_4713 0.20 +0.1 24,966 - Ac3H11_4713 0.20 +3.2 24,966 - Ac3H11_4713 0.20 +3.0 24,966 - Ac3H11_4713 0.20 +1.6 25,028 + Ac3H11_4713 0.28 +4.1 25,028 + Ac3H11_4713 0.28 -0.4 25,029 - Ac3H11_4713 0.28 -0.7 25,031 + Ac3H11_4713 0.29 -1.5 25,031 + Ac3H11_4713 0.29 +1.8 25,031 + Ac3H11_4713 0.29 +3.6 25,031 + Ac3H11_4713 0.29 +0.2 25,031 + Ac3H11_4713 0.29 +1.8 25,031 + Ac3H11_4713 0.29 +3.1 25,031 + Ac3H11_4713 0.29 +5.1 25,032 - Ac3H11_4713 0.29 +1.0 25,032 - Ac3H11_4713 0.29 +2.7 25,032 - Ac3H11_4713 0.29 -0.2 25,032 - Ac3H11_4713 0.29 +0.8 25,032 - Ac3H11_4713 0.29 +2.3 25,032 - Ac3H11_4713 0.29 +2.7 25,032 - Ac3H11_4713 0.29 +3.0 25,032 - Ac3H11_4713 0.29 +2.3 25,032 - Ac3H11_4713 0.29 +2.8 25,032 - Ac3H11_4713 0.29 +1.4 25,046 + Ac3H11_4713 0.31 +3.4 25,582 + +2.6 25,582 + +4.6 25,582 + +3.6 25,582 + +1.3 25,582 + -0.5 25,582 + -0.9 25,582 + +3.2 25,583 - +3.6 25,583 - +1.1 25,583 - +4.2 25,583 - -0.2 25,742 + Ac3H11_4714 0.24 +0.8 25,742 + Ac3H11_4714 0.24 +1.4
Or see this region's nucleotide sequence