Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_44 and Ac3H11_45 overlap by 30 nucleotides Ac3H11_45 and Ac3H11_46 are separated by 127 nucleotides Ac3H11_46 and Ac3H11_47 are separated by 47 nucleotides Ac3H11_47 and Ac3H11_48 are separated by 99 nucleotides
Ac3H11_44 - TPR repeat precursor, at 41,082 to 42,158
_44
Ac3H11_45 - hypothetical protein, at 42,129 to 42,263
_45
Ac3H11_46 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 42,391 to 43,764
_46
Ac3H11_47 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 43,812 to 44,486
_47
Ac3H11_48 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 44,586 to 45,563
_48
Position (kb)
42
43
44 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 41.471 kb on + strand, within Ac3H11_44 at 41.472 kb on - strand, within Ac3H11_44 at 41.719 kb on + strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.845 kb on + strand, within Ac3H11_44 at 41.846 kb on - strand, within Ac3H11_44 at 41.866 kb on + strand, within Ac3H11_44 at 41.866 kb on + strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.887 kb on + strand, within Ac3H11_44 at 42.068 kb on - strand at 42.068 kb on - strand at 42.068 kb on - strand at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.220 kb on + strand, within Ac3H11_45 at 42.220 kb on + strand, within Ac3H11_45 at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.329 kb on + strand at 42.329 kb on + strand at 43.824 kb on - strand at 43.982 kb on + strand, within Ac3H11_47 at 44.151 kb on + strand, within Ac3H11_47 at 44.152 kb on - strand, within Ac3H11_47 at 44.295 kb on + strand, within Ac3H11_47 at 44.314 kb on + strand, within Ac3H11_47 at 44.361 kb on + strand, within Ac3H11_47 at 44.361 kb on + strand, within Ac3H11_47 at 44.445 kb on + strand at 44.481 kb on + strand at 44.481 kb on + strand at 44.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 41,471 + Ac3H11_44 0.36 +0.8 41,472 - Ac3H11_44 0.36 -0.9 41,719 + Ac3H11_44 0.59 -0.4 41,720 - Ac3H11_44 0.59 -2.0 41,720 - Ac3H11_44 0.59 +0.2 41,720 - Ac3H11_44 0.59 -0.8 41,720 - Ac3H11_44 0.59 +0.9 41,720 - Ac3H11_44 0.59 -1.4 41,743 + Ac3H11_44 0.61 -1.6 41,743 + Ac3H11_44 0.61 -1.7 41,743 + Ac3H11_44 0.61 -1.2 41,743 + Ac3H11_44 0.61 -3.1 41,743 + Ac3H11_44 0.61 -1.3 41,743 + Ac3H11_44 0.61 +0.6 41,744 - Ac3H11_44 0.61 -1.2 41,744 - Ac3H11_44 0.61 -0.7 41,744 - Ac3H11_44 0.61 -1.4 41,744 - Ac3H11_44 0.61 -0.0 41,744 - Ac3H11_44 0.61 -1.9 41,744 - Ac3H11_44 0.61 -0.7 41,744 - Ac3H11_44 0.61 +0.1 41,744 - Ac3H11_44 0.61 -1.7 41,776 + Ac3H11_44 0.64 +0.9 41,776 + Ac3H11_44 0.64 -0.7 41,776 + Ac3H11_44 0.64 -0.5 41,845 + Ac3H11_44 0.71 -2.1 41,846 - Ac3H11_44 0.71 -0.2 41,866 + Ac3H11_44 0.73 -0.8 41,866 + Ac3H11_44 0.73 +0.8 41,867 - Ac3H11_44 0.73 +1.5 41,867 - Ac3H11_44 0.73 -1.7 41,867 - Ac3H11_44 0.73 -0.8 41,867 - Ac3H11_44 0.73 +0.3 41,867 - Ac3H11_44 0.73 -0.8 41,867 - Ac3H11_44 0.73 -1.7 41,887 + Ac3H11_44 0.75 -1.3 42,068 - +0.3 42,068 - -0.4 42,068 - -1.9 42,203 + Ac3H11_45 0.55 -1.1 42,203 + Ac3H11_45 0.55 -0.1 42,203 + Ac3H11_45 0.55 -2.3 42,203 + Ac3H11_45 0.55 -0.2 42,203 + Ac3H11_45 0.55 -0.2 42,203 + Ac3H11_45 0.55 -0.1 42,203 + Ac3H11_45 0.55 -1.0 42,203 + Ac3H11_45 0.55 -0.5 42,203 + Ac3H11_45 0.55 +0.3 42,203 + Ac3H11_45 0.55 +0.5 42,203 + Ac3H11_45 0.55 -0.1 42,203 + Ac3H11_45 0.55 -0.4 42,203 + Ac3H11_45 0.55 +1.5 42,203 + Ac3H11_45 0.55 +0.6 42,204 - Ac3H11_45 0.56 -0.2 42,204 - Ac3H11_45 0.56 -0.4 42,204 - Ac3H11_45 0.56 -0.9 42,204 - Ac3H11_45 0.56 -3.2 42,204 - Ac3H11_45 0.56 +0.5 42,204 - Ac3H11_45 0.56 -1.0 42,204 - Ac3H11_45 0.56 +0.6 42,204 - Ac3H11_45 0.56 +0.1 42,204 - Ac3H11_45 0.56 +0.3 42,204 - Ac3H11_45 0.56 -0.3 42,204 - Ac3H11_45 0.56 -0.2 42,204 - Ac3H11_45 0.56 +0.1 42,204 - Ac3H11_45 0.56 +0.5 42,204 - Ac3H11_45 0.56 -1.6 42,204 - Ac3H11_45 0.56 -0.1 42,205 + Ac3H11_45 0.56 +4.9 42,205 + Ac3H11_45 0.56 +0.4 42,205 + Ac3H11_45 0.56 +4.3 42,205 + Ac3H11_45 0.56 +1.2 42,205 + Ac3H11_45 0.56 +3.7 42,205 + Ac3H11_45 0.56 +3.4 42,205 + Ac3H11_45 0.56 +1.4 42,205 + Ac3H11_45 0.56 +3.4 42,220 + Ac3H11_45 0.67 +2.8 42,220 + Ac3H11_45 0.67 -0.7 42,272 + -0.2 42,272 + +0.9 42,272 + -0.4 42,272 + -1.4 42,272 + -0.3 42,272 + +0.4 42,272 + +1.0 42,272 + -0.1 42,272 + -1.4 42,272 + -0.9 42,329 + +4.7 42,329 + +4.5 43,824 - -0.8 43,982 + Ac3H11_47 0.25 +0.7 44,151 + Ac3H11_47 0.50 +2.1 44,152 - Ac3H11_47 0.50 +0.5 44,295 + Ac3H11_47 0.72 +1.2 44,314 + Ac3H11_47 0.74 +1.2 44,361 + Ac3H11_47 0.81 +2.2 44,361 + Ac3H11_47 0.81 -0.1 44,445 + +0.8 44,481 + -0.5 44,481 + +1.5 44,481 + +1.3
Or see this region's nucleotide sequence