Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4597 and Ac3H11_4598 are separated by 176 nucleotides Ac3H11_4598 and Ac3H11_4599 are separated by 394 nucleotides Ac3H11_4599 and Ac3H11_4600 overlap by 4 nucleotides
Ac3H11_4597 - Copper tolerance protein, at 197,696 to 198,949
_4597
Ac3H11_4598 - hypothetical protein, at 199,126 to 199,335
_4598
Ac3H11_4599 - DNA-binding heavy metal response regulator, at 199,730 to 200,275
_4599
Ac3H11_4600 - Heavy metal sensor histidine kinase, at 200,272 to 201,678
_4600
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 198.793 kb on + strand, within Ac3H11_4597 at 198.793 kb on + strand, within Ac3H11_4597 at 198.793 kb on + strand, within Ac3H11_4597 at 198.793 kb on + strand, within Ac3H11_4597 at 198.793 kb on + strand, within Ac3H11_4597 at 198.794 kb on - strand, within Ac3H11_4597 at 198.875 kb on - strand at 198.875 kb on - strand at 198.982 kb on + strand at 198.982 kb on + strand at 198.982 kb on + strand at 198.982 kb on + strand at 198.982 kb on + strand at 198.982 kb on + strand at 198.983 kb on - strand at 198.983 kb on - strand at 198.983 kb on - strand at 199.094 kb on + strand at 199.094 kb on + strand at 199.094 kb on + strand at 199.094 kb on + strand at 199.095 kb on - strand at 199.095 kb on - strand at 199.095 kb on - strand at 199.095 kb on - strand at 199.095 kb on - strand at 199.120 kb on + strand at 199.121 kb on - strand at 199.215 kb on - strand, within Ac3H11_4598 at 199.215 kb on - strand, within Ac3H11_4598 at 199.215 kb on - strand, within Ac3H11_4598 at 199.287 kb on - strand, within Ac3H11_4598 at 199.296 kb on - strand, within Ac3H11_4598 at 199.296 kb on - strand, within Ac3H11_4598 at 199.296 kb on - strand, within Ac3H11_4598 at 199.328 kb on + strand at 199.328 kb on + strand at 199.329 kb on - strand at 199.416 kb on + strand at 199.416 kb on + strand at 199.416 kb on + strand at 199.443 kb on + strand at 199.443 kb on + strand at 199.497 kb on + strand at 199.501 kb on + strand at 199.501 kb on + strand at 199.501 kb on + strand at 199.501 kb on + strand at 199.513 kb on + strand at 199.513 kb on + strand at 199.513 kb on + strand at 199.513 kb on + strand at 199.514 kb on - strand at 199.514 kb on - strand at 199.514 kb on - strand at 199.514 kb on - strand at 199.514 kb on - strand at 199.514 kb on - strand at 199.596 kb on + strand at 199.596 kb on + strand at 199.596 kb on + strand at 199.596 kb on + strand at 199.597 kb on - strand at 199.631 kb on + strand at 199.632 kb on - strand at 199.632 kb on - strand at 199.721 kb on + strand at 199.721 kb on + strand at 199.722 kb on - strand at 199.722 kb on - strand at 199.722 kb on - strand at 199.722 kb on - strand at 199.722 kb on - strand at 199.783 kb on + strand at 199.783 kb on + strand at 199.783 kb on + strand at 199.783 kb on + strand at 199.783 kb on + strand at 199.784 kb on - strand at 199.784 kb on - strand at 199.815 kb on + strand, within Ac3H11_4599 at 199.815 kb on + strand, within Ac3H11_4599 at 199.815 kb on + strand, within Ac3H11_4599 at 199.815 kb on + strand, within Ac3H11_4599 at 199.816 kb on - strand, within Ac3H11_4599 at 199.816 kb on - strand, within Ac3H11_4599 at 199.860 kb on + strand, within Ac3H11_4599 at 199.860 kb on + strand, within Ac3H11_4599 at 199.860 kb on + strand, within Ac3H11_4599 at 199.860 kb on + strand, within Ac3H11_4599 at 199.861 kb on - strand, within Ac3H11_4599 at 199.861 kb on - strand, within Ac3H11_4599 at 199.880 kb on + strand, within Ac3H11_4599 at 199.880 kb on + strand, within Ac3H11_4599 at 199.881 kb on - strand, within Ac3H11_4599 at 199.932 kb on + strand, within Ac3H11_4599 at 199.932 kb on + strand, within Ac3H11_4599 at 199.932 kb on + strand, within Ac3H11_4599 at 199.933 kb on - strand, within Ac3H11_4599 at 199.933 kb on - strand, within Ac3H11_4599 at 199.933 kb on - strand, within Ac3H11_4599 at 199.967 kb on - strand, within Ac3H11_4599 at 199.967 kb on - strand, within Ac3H11_4599 at 200.131 kb on + strand, within Ac3H11_4599 at 200.131 kb on + strand, within Ac3H11_4599 at 200.132 kb on - strand, within Ac3H11_4599 at 200.204 kb on + strand, within Ac3H11_4599 at 200.205 kb on - strand, within Ac3H11_4599 at 200.205 kb on - strand, within Ac3H11_4599 at 200.205 kb on - strand, within Ac3H11_4599 at 200.243 kb on + strand at 200.243 kb on + strand at 200.243 kb on + strand at 200.244 kb on - strand at 200.244 kb on - strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.316 kb on + strand at 200.317 kb on - strand at 200.317 kb on - strand at 200.370 kb on + strand at 200.442 kb on - strand, within Ac3H11_4600 at 200.442 kb on - strand, within Ac3H11_4600 at 200.705 kb on + strand, within Ac3H11_4600 at 200.705 kb on + strand, within Ac3H11_4600 at 200.711 kb on + strand, within Ac3H11_4600 at 200.711 kb on + strand, within Ac3H11_4600 at 200.711 kb on + strand, within Ac3H11_4600 at 200.711 kb on + strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.712 kb on - strand, within Ac3H11_4600 at 200.740 kb on + strand, within Ac3H11_4600 at 200.740 kb on + strand, within Ac3H11_4600 at 200.740 kb on + strand, within Ac3H11_4600 at 200.740 kb on + strand, within Ac3H11_4600 at 200.740 kb on + strand, within Ac3H11_4600 at 200.741 kb on - strand, within Ac3H11_4600 at 200.767 kb on + strand, within Ac3H11_4600 at 200.767 kb on + strand, within Ac3H11_4600 at 200.768 kb on - strand, within Ac3H11_4600 at 200.787 kb on - strand, within Ac3H11_4600 at 201.063 kb on + strand, within Ac3H11_4600 at 201.063 kb on + strand, within Ac3H11_4600 at 201.088 kb on + strand, within Ac3H11_4600 at 201.088 kb on + strand, within Ac3H11_4600 at 201.088 kb on + strand, within Ac3H11_4600 at 201.089 kb on - strand, within Ac3H11_4600 at 201.089 kb on - strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.098 kb on + strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.099 kb on - strand, within Ac3H11_4600 at 201.121 kb on + strand, within Ac3H11_4600 at 201.121 kb on + strand, within Ac3H11_4600 at 201.121 kb on + strand, within Ac3H11_4600 at 201.121 kb on + strand, within Ac3H11_4600 at 201.121 kb on + strand, within Ac3H11_4600 at 201.183 kb on + strand, within Ac3H11_4600 at 201.183 kb on + strand, within Ac3H11_4600 at 201.183 kb on + strand, within Ac3H11_4600 at 201.183 kb on + strand, within Ac3H11_4600 at 201.183 kb on + strand, within Ac3H11_4600 at 201.184 kb on - strand, within Ac3H11_4600 at 201.184 kb on - strand, within Ac3H11_4600 at 201.184 kb on - strand, within Ac3H11_4600 at 201.184 kb on - strand, within Ac3H11_4600
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 198,793 + Ac3H11_4597 0.87 -1.1 198,793 + Ac3H11_4597 0.87 -1.0 198,793 + Ac3H11_4597 0.87 +0.5 198,793 + Ac3H11_4597 0.87 -1.1 198,793 + Ac3H11_4597 0.87 -0.3 198,794 - Ac3H11_4597 0.88 +0.5 198,875 - +0.4 198,875 - -0.3 198,982 + +0.8 198,982 + -2.4 198,982 + -1.0 198,982 + +0.4 198,982 + -0.1 198,982 + -0.3 198,983 - -0.1 198,983 - -0.8 198,983 - -2.5 199,094 + +0.3 199,094 + +0.2 199,094 + -2.0 199,094 + +1.0 199,095 - +2.7 199,095 - +0.1 199,095 - -2.1 199,095 - -1.0 199,095 - +0.7 199,120 + +0.3 199,121 - -0.7 199,215 - Ac3H11_4598 0.42 +0.5 199,215 - Ac3H11_4598 0.42 -1.9 199,215 - Ac3H11_4598 0.42 +0.7 199,287 - Ac3H11_4598 0.77 -1.0 199,296 - Ac3H11_4598 0.81 -0.6 199,296 - Ac3H11_4598 0.81 +0.7 199,296 - Ac3H11_4598 0.81 +2.2 199,328 + +0.7 199,328 + +1.5 199,329 - -1.7 199,416 + +0.2 199,416 + -1.1 199,416 + -0.1 199,443 + +1.6 199,443 + -0.9 199,497 + +0.2 199,501 + -1.6 199,501 + +1.5 199,501 + +1.1 199,501 + +0.3 199,513 + +1.4 199,513 + +0.5 199,513 + -1.1 199,513 + -1.9 199,514 - -0.2 199,514 - -0.5 199,514 - +1.1 199,514 - -0.7 199,514 - +0.7 199,514 - +1.1 199,596 + +0.7 199,596 + -0.9 199,596 + -0.0 199,596 + -0.1 199,597 - +1.1 199,631 + +0.6 199,632 - +0.9 199,632 - +0.2 199,721 + +2.2 199,721 + +0.6 199,722 - -2.1 199,722 - -0.4 199,722 - +0.3 199,722 - -0.6 199,722 - -0.4 199,783 + -0.8 199,783 + -0.3 199,783 + -0.4 199,783 + -0.1 199,783 + -0.2 199,784 - -0.1 199,784 - -1.3 199,815 + Ac3H11_4599 0.16 -0.5 199,815 + Ac3H11_4599 0.16 -1.7 199,815 + Ac3H11_4599 0.16 +0.2 199,815 + Ac3H11_4599 0.16 +0.5 199,816 - Ac3H11_4599 0.16 +0.2 199,816 - Ac3H11_4599 0.16 +0.3 199,860 + Ac3H11_4599 0.24 +0.7 199,860 + Ac3H11_4599 0.24 +2.6 199,860 + Ac3H11_4599 0.24 +0.1 199,860 + Ac3H11_4599 0.24 -0.1 199,861 - Ac3H11_4599 0.24 +1.1 199,861 - Ac3H11_4599 0.24 +2.9 199,880 + Ac3H11_4599 0.27 -1.1 199,880 + Ac3H11_4599 0.27 +1.7 199,881 - Ac3H11_4599 0.28 -0.3 199,932 + Ac3H11_4599 0.37 -1.5 199,932 + Ac3H11_4599 0.37 -0.2 199,932 + Ac3H11_4599 0.37 +0.1 199,933 - Ac3H11_4599 0.37 +0.7 199,933 - Ac3H11_4599 0.37 -0.1 199,933 - Ac3H11_4599 0.37 -0.1 199,967 - Ac3H11_4599 0.43 +1.8 199,967 - Ac3H11_4599 0.43 -0.8 200,131 + Ac3H11_4599 0.73 -2.4 200,131 + Ac3H11_4599 0.73 -1.3 200,132 - Ac3H11_4599 0.74 -1.5 200,204 + Ac3H11_4599 0.87 -1.2 200,205 - Ac3H11_4599 0.87 -0.2 200,205 - Ac3H11_4599 0.87 +0.1 200,205 - Ac3H11_4599 0.87 -0.1 200,243 + -0.6 200,243 + -0.3 200,243 + +1.2 200,244 - -1.6 200,244 - -2.2 200,316 + -0.1 200,316 + +1.2 200,316 + -1.2 200,316 + -0.9 200,317 - -1.0 200,317 - +0.2 200,370 + -0.3 200,442 - Ac3H11_4600 0.12 +0.2 200,442 - Ac3H11_4600 0.12 +0.8 200,705 + Ac3H11_4600 0.31 +2.9 200,705 + Ac3H11_4600 0.31 -1.3 200,711 + Ac3H11_4600 0.31 +1.3 200,711 + Ac3H11_4600 0.31 -0.7 200,711 + Ac3H11_4600 0.31 +1.7 200,711 + Ac3H11_4600 0.31 +0.1 200,712 - Ac3H11_4600 0.31 +1.8 200,712 - Ac3H11_4600 0.31 -1.0 200,712 - Ac3H11_4600 0.31 -2.3 200,712 - Ac3H11_4600 0.31 -0.8 200,712 - Ac3H11_4600 0.31 -0.5 200,712 - Ac3H11_4600 0.31 -0.9 200,712 - Ac3H11_4600 0.31 -2.3 200,712 - Ac3H11_4600 0.31 +0.8 200,740 + Ac3H11_4600 0.33 +0.1 200,740 + Ac3H11_4600 0.33 -0.2 200,740 + Ac3H11_4600 0.33 -1.9 200,740 + Ac3H11_4600 0.33 +2.3 200,740 + Ac3H11_4600 0.33 +1.3 200,741 - Ac3H11_4600 0.33 +0.2 200,767 + Ac3H11_4600 0.35 -0.3 200,767 + Ac3H11_4600 0.35 +1.2 200,768 - Ac3H11_4600 0.35 -2.6 200,787 - Ac3H11_4600 0.37 +0.2 201,063 + Ac3H11_4600 0.56 -1.2 201,063 + Ac3H11_4600 0.56 +0.7 201,088 + Ac3H11_4600 0.58 -0.3 201,088 + Ac3H11_4600 0.58 -0.1 201,088 + Ac3H11_4600 0.58 +0.1 201,089 - Ac3H11_4600 0.58 -0.4 201,089 - Ac3H11_4600 0.58 +0.0 201,098 + Ac3H11_4600 0.59 -0.9 201,098 + Ac3H11_4600 0.59 +0.6 201,098 + Ac3H11_4600 0.59 +0.6 201,098 + Ac3H11_4600 0.59 -2.4 201,098 + Ac3H11_4600 0.59 -1.4 201,098 + Ac3H11_4600 0.59 -1.6 201,098 + Ac3H11_4600 0.59 -2.8 201,098 + Ac3H11_4600 0.59 +0.9 201,099 - Ac3H11_4600 0.59 +0.2 201,099 - Ac3H11_4600 0.59 -1.3 201,099 - Ac3H11_4600 0.59 +0.7 201,099 - Ac3H11_4600 0.59 -3.2 201,099 - Ac3H11_4600 0.59 +0.7 201,099 - Ac3H11_4600 0.59 +0.8 201,099 - Ac3H11_4600 0.59 +1.0 201,099 - Ac3H11_4600 0.59 -1.9 201,099 - Ac3H11_4600 0.59 -0.2 201,121 + Ac3H11_4600 0.60 +1.1 201,121 + Ac3H11_4600 0.60 -0.8 201,121 + Ac3H11_4600 0.60 +1.1 201,121 + Ac3H11_4600 0.60 -0.8 201,121 + Ac3H11_4600 0.60 -1.5 201,183 + Ac3H11_4600 0.65 -1.2 201,183 + Ac3H11_4600 0.65 +1.1 201,183 + Ac3H11_4600 0.65 +0.1 201,183 + Ac3H11_4600 0.65 -0.0 201,183 + Ac3H11_4600 0.65 +0.7 201,184 - Ac3H11_4600 0.65 -0.3 201,184 - Ac3H11_4600 0.65 +0.8 201,184 - Ac3H11_4600 0.65 -1.5 201,184 - Ac3H11_4600 0.65 -1.4
Or see this region's nucleotide sequence