Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3698 and Ac3H11_3699 are separated by 9 nucleotides Ac3H11_3699 and Ac3H11_3700 are separated by 20 nucleotides Ac3H11_3700 and Ac3H11_3701 are separated by 32 nucleotides Ac3H11_3701 and Ac3H11_3702 are separated by 213 nucleotides
Ac3H11_3698 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 56,431 to 57,483
_3698
Ac3H11_3699 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 57,493 to 57,957
_3699
Ac3H11_3700 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 57,978 to 60,230
_3700
Ac3H11_3701 - hypothetical protein, at 60,263 to 60,382
_3701
Ac3H11_3702 - Urea ABC transporter, urea binding protein, at 60,596 to 61,858
_3702
Position (kb) 57
58
59
60
61 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.986 kb on + strand, within Ac3H11_3698 at 56.986 kb on + strand, within Ac3H11_3698 at 56.987 kb on - strand, within Ac3H11_3698 at 56.987 kb on - strand, within Ac3H11_3698 at 57.409 kb on + strand at 57.409 kb on + strand at 57.410 kb on - strand at 57.410 kb on - strand at 57.410 kb on - strand at 57.481 kb on + strand at 57.481 kb on + strand at 57.548 kb on + strand, within Ac3H11_3699 at 57.549 kb on - strand, within Ac3H11_3699 at 58.843 kb on + strand, within Ac3H11_3700 at 58.843 kb on + strand, within Ac3H11_3700 at 58.936 kb on + strand, within Ac3H11_3700 at 58.936 kb on + strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 59.381 kb on - strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 60.374 kb on - strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.398 kb on + strand at 60.480 kb on - strand at 60.524 kb on + strand at 60.524 kb on + strand at 60.524 kb on + strand at 60.525 kb on - strand at 60.576 kb on + strand at 60.576 kb on + strand at 60.576 kb on + strand at 60.577 kb on - strand at 60.577 kb on - strand at 60.577 kb on - strand at 60.608 kb on + strand at 60.609 kb on - strand at 60.984 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.062 kb on - strand, within Ac3H11_3702 at 61.118 kb on + strand, within Ac3H11_3702 at 61.118 kb on + strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.157 kb on + strand, within Ac3H11_3702 at 61.157 kb on + strand, within Ac3H11_3702 at 61.202 kb on + strand, within Ac3H11_3702 at 61.202 kb on + strand, within Ac3H11_3702 at 61.203 kb on - strand, within Ac3H11_3702 at 61.203 kb on - strand, within Ac3H11_3702 at 61.227 kb on - strand, within Ac3H11_3702
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 56,986 + Ac3H11_3698 0.53 -0.4 56,986 + Ac3H11_3698 0.53 -0.9 56,987 - Ac3H11_3698 0.53 -0.2 56,987 - Ac3H11_3698 0.53 +0.3 57,409 + +0.7 57,409 + -0.3 57,410 - +0.6 57,410 - -1.4 57,410 - +0.1 57,481 + -0.2 57,481 + +0.6 57,548 + Ac3H11_3699 0.12 -0.6 57,549 - Ac3H11_3699 0.12 -1.9 58,843 + Ac3H11_3700 0.38 -1.3 58,843 + Ac3H11_3700 0.38 -2.6 58,936 + Ac3H11_3700 0.43 -0.7 58,936 + Ac3H11_3700 0.43 -1.0 58,937 - Ac3H11_3700 0.43 -1.1 58,937 - Ac3H11_3700 0.43 +0.2 58,937 - Ac3H11_3700 0.43 -1.2 58,937 - Ac3H11_3700 0.43 +0.9 59,381 - Ac3H11_3700 0.62 +1.1 59,947 + Ac3H11_3700 0.87 -1.1 59,947 + Ac3H11_3700 0.87 -0.4 59,947 + Ac3H11_3700 0.87 -0.0 59,948 - Ac3H11_3700 0.87 +0.1 59,948 - Ac3H11_3700 0.87 -0.0 59,948 - Ac3H11_3700 0.87 +0.4 59,948 - Ac3H11_3700 0.87 -0.9 59,948 - Ac3H11_3700 0.87 -2.7 60,374 - +0.6 60,383 + -0.3 60,383 + -1.0 60,383 + -0.8 60,383 + +0.7 60,398 + +1.8 60,480 - +0.7 60,524 + -0.8 60,524 + -0.4 60,524 + -0.4 60,525 - +1.9 60,576 + -1.8 60,576 + -0.4 60,576 + -0.0 60,577 - -2.2 60,577 - -0.3 60,577 - +0.6 60,608 + -0.6 60,609 - -0.8 60,984 - Ac3H11_3702 0.31 +1.8 61,017 - Ac3H11_3702 0.33 -2.0 61,017 - Ac3H11_3702 0.33 -1.2 61,017 - Ac3H11_3702 0.33 +1.1 61,017 - Ac3H11_3702 0.33 +0.4 61,017 - Ac3H11_3702 0.33 -1.1 61,062 - Ac3H11_3702 0.37 +2.0 61,118 + Ac3H11_3702 0.41 -0.6 61,118 + Ac3H11_3702 0.41 -0.0 61,119 - Ac3H11_3702 0.41 -0.4 61,119 - Ac3H11_3702 0.41 +0.6 61,119 - Ac3H11_3702 0.41 +0.6 61,119 - Ac3H11_3702 0.41 +0.8 61,157 + Ac3H11_3702 0.44 +1.8 61,157 + Ac3H11_3702 0.44 -1.3 61,202 + Ac3H11_3702 0.48 -0.6 61,202 + Ac3H11_3702 0.48 -0.7 61,203 - Ac3H11_3702 0.48 -0.2 61,203 - Ac3H11_3702 0.48 +1.2 61,227 - Ac3H11_3702 0.50 +0.2
Or see this region's nucleotide sequence