Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3074 and Ac3H11_3075 are separated by 193 nucleotides Ac3H11_3075 and Ac3H11_3076 are separated by 55 nucleotides Ac3H11_3076 and Ac3H11_3077 are separated by 46 nucleotides Ac3H11_3077 and Ac3H11_3078 overlap by 9 nucleotides
Ac3H11_3074 - hypothetical protein, at 99,383 to 99,502
_3074
Ac3H11_3075 - hypothetical protein, at 99,696 to 99,809
_3075
Ac3H11_3076 - Antibiotic biosynthesis monooxygenase, at 99,865 to 100,164
_3076
Ac3H11_3077 - hypothetical protein, at 100,211 to 100,540
_3077
Ac3H11_3078 - ATP-dependent DNA helicase UvrD/PcrA, at 100,532 to 102,562
_3078
Position (kb) 99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 98.750 kb on + strand at 98.751 kb on - strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.020 kb on - strand at 99.020 kb on - strand at 99.020 kb on - strand at 99.020 kb on - strand at 99.101 kb on + strand at 99.101 kb on + strand at 99.102 kb on - strand at 99.102 kb on - strand at 99.102 kb on - strand at 99.298 kb on + strand at 99.298 kb on + strand at 99.298 kb on + strand at 99.298 kb on + strand at 99.298 kb on + strand at 99.299 kb on - strand at 99.299 kb on - strand at 99.299 kb on - strand at 99.299 kb on - strand at 99.354 kb on + strand at 99.355 kb on - strand at 99.355 kb on - strand at 99.355 kb on - strand at 99.355 kb on - strand at 99.407 kb on + strand, within Ac3H11_3074 at 99.407 kb on + strand, within Ac3H11_3074 at 99.407 kb on + strand, within Ac3H11_3074 at 99.407 kb on + strand, within Ac3H11_3074 at 99.408 kb on - strand, within Ac3H11_3074 at 99.408 kb on - strand, within Ac3H11_3074 at 99.408 kb on - strand, within Ac3H11_3074 at 99.408 kb on - strand, within Ac3H11_3074 at 99.408 kb on - strand, within Ac3H11_3074 at 99.416 kb on + strand, within Ac3H11_3074 at 99.416 kb on + strand, within Ac3H11_3074 at 99.416 kb on + strand, within Ac3H11_3074 at 99.417 kb on - strand, within Ac3H11_3074 at 99.418 kb on + strand, within Ac3H11_3074 at 99.418 kb on + strand, within Ac3H11_3074 at 99.418 kb on + strand, within Ac3H11_3074 at 99.418 kb on + strand, within Ac3H11_3074 at 99.418 kb on + strand, within Ac3H11_3074 at 99.419 kb on - strand, within Ac3H11_3074 at 99.432 kb on - strand, within Ac3H11_3074 at 99.432 kb on - strand, within Ac3H11_3074 at 99.497 kb on + strand at 99.497 kb on + strand at 99.498 kb on - strand at 99.631 kb on + strand at 99.631 kb on + strand at 99.632 kb on - strand at 99.632 kb on - strand at 99.632 kb on - strand at 99.632 kb on - strand at 99.637 kb on + strand at 99.637 kb on + strand at 99.637 kb on + strand at 99.637 kb on + strand at 99.637 kb on + strand at 99.637 kb on + strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.667 kb on + strand at 99.668 kb on - strand at 99.668 kb on - strand at 99.668 kb on - strand at 99.668 kb on - strand at 99.668 kb on - strand at 99.668 kb on - strand at 99.688 kb on - strand at 99.688 kb on - strand at 99.688 kb on - strand at 99.749 kb on + strand, within Ac3H11_3075 at 99.750 kb on - strand, within Ac3H11_3075 at 99.759 kb on + strand, within Ac3H11_3075 at 99.759 kb on + strand, within Ac3H11_3075 at 99.760 kb on - strand, within Ac3H11_3075 at 99.760 kb on - strand, within Ac3H11_3075 at 99.760 kb on - strand, within Ac3H11_3075 at 99.903 kb on + strand, within Ac3H11_3076 at 99.903 kb on + strand, within Ac3H11_3076 at 100.135 kb on + strand at 100.135 kb on + strand at 100.135 kb on + strand at 100.135 kb on + strand at 100.135 kb on + strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.136 kb on - strand at 100.171 kb on + strand at 100.171 kb on + strand at 100.172 kb on - strand at 100.179 kb on + strand at 100.180 kb on - strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.212 kb on + strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.213 kb on - strand at 100.216 kb on + strand at 100.216 kb on + strand at 100.216 kb on + strand at 100.216 kb on + strand at 100.217 kb on - strand at 100.217 kb on - strand at 100.217 kb on - strand at 100.217 kb on - strand at 100.226 kb on + strand at 100.226 kb on + strand at 100.226 kb on + strand at 100.226 kb on + strand at 100.226 kb on + strand at 100.227 kb on - strand at 100.227 kb on - strand at 100.227 kb on - strand at 100.227 kb on - strand at 100.227 kb on - strand at 100.256 kb on - strand, within Ac3H11_3077 at 100.256 kb on - strand, within Ac3H11_3077 at 100.342 kb on + strand, within Ac3H11_3077 at 100.342 kb on + strand, within Ac3H11_3077 at 100.343 kb on - strand, within Ac3H11_3077 at 100.343 kb on - strand, within Ac3H11_3077 at 100.343 kb on - strand, within Ac3H11_3077 at 100.343 kb on - strand, within Ac3H11_3077 at 100.387 kb on - strand, within Ac3H11_3077 at 100.387 kb on - strand, within Ac3H11_3077 at 100.387 kb on - strand, within Ac3H11_3077 at 100.730 kb on + strand at 100.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 98,750 + +1.4 98,751 - +0.5 99,019 + +0.8 99,019 + +0.3 99,019 + -0.2 99,020 - -0.3 99,020 - +0.3 99,020 - +0.1 99,020 - -0.3 99,101 + -1.7 99,101 + -0.7 99,102 - +0.7 99,102 - +1.1 99,102 - -0.5 99,298 + -0.2 99,298 + -2.1 99,298 + -0.7 99,298 + +1.3 99,298 + -0.3 99,299 - +1.2 99,299 - -1.8 99,299 - -1.4 99,299 - +0.1 99,354 + -0.6 99,355 - -0.7 99,355 - +0.5 99,355 - -0.8 99,355 - +0.2 99,407 + Ac3H11_3074 0.20 +0.8 99,407 + Ac3H11_3074 0.20 -0.6 99,407 + Ac3H11_3074 0.20 +0.5 99,407 + Ac3H11_3074 0.20 -0.5 99,408 - Ac3H11_3074 0.21 +0.8 99,408 - Ac3H11_3074 0.21 -0.0 99,408 - Ac3H11_3074 0.21 -0.7 99,408 - Ac3H11_3074 0.21 -1.7 99,408 - Ac3H11_3074 0.21 +0.0 99,416 + Ac3H11_3074 0.28 -0.6 99,416 + Ac3H11_3074 0.28 -0.3 99,416 + Ac3H11_3074 0.28 +0.0 99,417 - Ac3H11_3074 0.28 +0.7 99,418 + Ac3H11_3074 0.29 +0.1 99,418 + Ac3H11_3074 0.29 +0.3 99,418 + Ac3H11_3074 0.29 +5.5 99,418 + Ac3H11_3074 0.29 -1.0 99,418 + Ac3H11_3074 0.29 +0.0 99,419 - Ac3H11_3074 0.30 +1.3 99,432 - Ac3H11_3074 0.41 -1.2 99,432 - Ac3H11_3074 0.41 -0.9 99,497 + +0.1 99,497 + -2.3 99,498 - -1.0 99,631 + -2.3 99,631 + +0.8 99,632 - +0.2 99,632 - -0.4 99,632 - +1.3 99,632 - -0.8 99,637 + -1.4 99,637 + -1.7 99,637 + +0.2 99,637 + -1.3 99,637 + -0.5 99,637 + -0.1 99,638 - +0.1 99,638 - +0.2 99,638 - -1.5 99,638 - +0.4 99,638 - -0.1 99,638 - -0.4 99,638 - +2.0 99,667 + +0.7 99,667 + +1.0 99,667 + +1.3 99,667 + +0.5 99,667 + -0.5 99,667 + +0.5 99,667 + +0.5 99,668 - +0.2 99,668 - -1.3 99,668 - +0.9 99,668 - +1.1 99,668 - -0.4 99,668 - -0.7 99,688 - -0.8 99,688 - +0.7 99,688 - +0.6 99,749 + Ac3H11_3075 0.46 -0.1 99,750 - Ac3H11_3075 0.47 -2.2 99,759 + Ac3H11_3075 0.55 +2.1 99,759 + Ac3H11_3075 0.55 -0.0 99,760 - Ac3H11_3075 0.56 -0.0 99,760 - Ac3H11_3075 0.56 -0.8 99,760 - Ac3H11_3075 0.56 -0.2 99,903 + Ac3H11_3076 0.13 -0.3 99,903 + Ac3H11_3076 0.13 -2.3 100,135 + -0.1 100,135 + -2.0 100,135 + +1.9 100,135 + -1.7 100,135 + -1.3 100,136 - +0.9 100,136 - -1.4 100,136 - +1.2 100,136 - -0.3 100,136 - -0.6 100,136 - -1.3 100,136 - -0.1 100,136 - +0.2 100,136 - -0.1 100,136 - -0.3 100,136 - -0.7 100,171 + -0.5 100,171 + +2.7 100,172 - +0.3 100,179 + +0.7 100,180 - +0.3 100,212 + -1.2 100,212 + -0.5 100,212 + -0.7 100,212 + -0.7 100,212 + +0.1 100,212 + -0.3 100,212 + +0.6 100,212 + +1.3 100,212 + -0.7 100,212 + +0.3 100,212 + -0.6 100,212 + +0.5 100,212 + -0.2 100,212 + -1.4 100,212 + +2.7 100,213 - +1.5 100,213 - -2.6 100,213 - -0.1 100,213 - -1.9 100,213 - -1.0 100,213 - -2.1 100,213 - -2.0 100,213 - +1.1 100,213 - +1.0 100,213 - -0.1 100,213 - +0.3 100,213 - +0.5 100,213 - +0.3 100,213 - -1.2 100,216 + -0.4 100,216 + -2.0 100,216 + -0.8 100,216 + -1.8 100,217 - -0.9 100,217 - +0.8 100,217 - -0.0 100,217 - -0.5 100,226 + -1.1 100,226 + -0.4 100,226 + +0.1 100,226 + -0.6 100,226 + -0.8 100,227 - -1.9 100,227 - -0.8 100,227 - -1.0 100,227 - -1.7 100,227 - +0.7 100,256 - Ac3H11_3077 0.14 +2.1 100,256 - Ac3H11_3077 0.14 +0.1 100,342 + Ac3H11_3077 0.40 -2.9 100,342 + Ac3H11_3077 0.40 -1.0 100,343 - Ac3H11_3077 0.40 +1.3 100,343 - Ac3H11_3077 0.40 +0.1 100,343 - Ac3H11_3077 0.40 +0.5 100,343 - Ac3H11_3077 0.40 +0.4 100,387 - Ac3H11_3077 0.53 +0.2 100,387 - Ac3H11_3077 0.53 -0.3 100,387 - Ac3H11_3077 0.53 +1.1 100,730 + +0.1 100,731 - +1.2
Or see this region's nucleotide sequence