Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1815 and Ac3H11_1816 are separated by 28 nucleotides Ac3H11_1816 and Ac3H11_1817 are separated by 55 nucleotides Ac3H11_1817 and Ac3H11_1818 are separated by 48 nucleotides Ac3H11_1818 and Ac3H11_1819 are separated by 29 nucleotides
Ac3H11_1815 - Phosphoglycerate mutase (EC 5.4.2.1), at 20,944 to 21,585
_1815
Ac3H11_1816 - Probable lipoprotein transmembrane, at 21,614 to 22,228
_1816
Ac3H11_1817 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 22,284 to 23,225
_1817
Ac3H11_1818 - Dihydroneopterin aldolase (EC 4.1.2.25), at 23,274 to 23,648
_1818
Ac3H11_1819 - FolM Alternative dihydrofolate reductase 1, at 23,678 to 24,463
_1819
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.709 kb on - strand, within Ac3H11_1816 at 21.759 kb on + strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.903 kb on + strand, within Ac3H11_1816 at 21.903 kb on + strand at 21.903 kb on + strand, within Ac3H11_1816 at 21.904 kb on - strand, within Ac3H11_1816 at 21.912 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816 at 22.086 kb on + strand, within Ac3H11_1816 at 22.086 kb on + strand, within Ac3H11_1816 at 22.087 kb on - strand, within Ac3H11_1816 at 22.408 kb on + strand, within Ac3H11_1817 at 22.408 kb on + strand, within Ac3H11_1817 at 22.408 kb on + strand, within Ac3H11_1817 at 22.409 kb on - strand, within Ac3H11_1817 at 22.618 kb on + strand, within Ac3H11_1817 at 22.618 kb on + strand, within Ac3H11_1817 at 22.619 kb on - strand, within Ac3H11_1817 at 22.787 kb on - strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.912 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.219 kb on + strand at 23.219 kb on + strand at 23.220 kb on - strand at 23.973 kb on + strand, within Ac3H11_1819 at 23.974 kb on - strand, within Ac3H11_1819
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 21,425 + Ac3H11_1815 0.75 +0.3 21,425 + Ac3H11_1815 0.75 +0.0 21,425 + Ac3H11_1815 0.75 -1.0 21,426 - Ac3H11_1815 0.75 +0.4 21,426 - Ac3H11_1815 0.75 +0.7 21,426 - Ac3H11_1815 0.75 -0.5 21,426 - Ac3H11_1815 0.75 +0.9 21,426 - Ac3H11_1815 0.75 -1.4 21,426 - Ac3H11_1815 0.75 +0.6 21,426 - Ac3H11_1815 0.75 -1.4 21,426 - Ac3H11_1815 0.75 +1.1 21,426 - Ac3H11_1815 0.75 -2.4 21,426 - Ac3H11_1815 0.75 +0.6 21,426 - Ac3H11_1815 0.75 +1.7 21,426 - Ac3H11_1815 0.75 +0.6 21,708 + Ac3H11_1816 0.15 -2.2 21,708 + Ac3H11_1816 0.15 +0.8 21,708 + Ac3H11_1816 0.15 +1.5 21,708 + Ac3H11_1816 0.15 -2.0 21,708 + Ac3H11_1816 0.15 +0.5 21,708 + Ac3H11_1816 0.15 +1.4 21,709 - Ac3H11_1816 0.15 -1.0 21,759 + Ac3H11_1816 0.24 +3.1 21,760 - Ac3H11_1816 0.24 -1.9 21,760 - Ac3H11_1816 0.24 +0.7 21,903 + Ac3H11_1816 0.47 -2.4 21,903 + +2.7 21,903 + Ac3H11_1816 0.47 +3.7 21,904 - Ac3H11_1816 0.47 -1.7 21,912 + Ac3H11_1816 0.48 -1.3 21,930 + Ac3H11_1816 0.51 +0.4 21,930 + Ac3H11_1816 0.51 +2.7 21,931 - Ac3H11_1816 0.52 +1.2 21,931 - Ac3H11_1816 0.52 -0.8 22,086 + Ac3H11_1816 0.77 +1.7 22,086 + Ac3H11_1816 0.77 +0.7 22,087 - Ac3H11_1816 0.77 +2.1 22,408 + Ac3H11_1817 0.13 -1.1 22,408 + Ac3H11_1817 0.13 -0.7 22,408 + Ac3H11_1817 0.13 -1.0 22,409 - Ac3H11_1817 0.13 -0.0 22,618 + Ac3H11_1817 0.35 +0.3 22,618 + Ac3H11_1817 0.35 -0.6 22,619 - Ac3H11_1817 0.36 +0.7 22,787 - Ac3H11_1817 0.53 -0.6 22,864 + Ac3H11_1817 0.62 -0.8 22,864 + Ac3H11_1817 0.62 -0.6 22,864 + Ac3H11_1817 0.62 -0.9 22,864 + Ac3H11_1817 0.62 +1.8 22,864 + Ac3H11_1817 0.62 -0.9 22,864 + Ac3H11_1817 0.62 -0.0 22,864 + Ac3H11_1817 0.62 -0.7 22,864 + Ac3H11_1817 0.62 -1.4 22,864 + Ac3H11_1817 0.62 -0.9 22,864 + Ac3H11_1817 0.62 -1.2 22,864 + Ac3H11_1817 0.62 +0.4 22,864 + Ac3H11_1817 0.62 -0.9 22,864 + Ac3H11_1817 0.62 +0.5 22,865 - Ac3H11_1817 0.62 +0.2 22,865 - Ac3H11_1817 0.62 +1.9 22,865 - Ac3H11_1817 0.62 +0.2 22,865 - Ac3H11_1817 0.62 +0.5 22,865 - Ac3H11_1817 0.62 -1.6 22,865 - Ac3H11_1817 0.62 +0.7 22,865 - Ac3H11_1817 0.62 -2.8 22,865 - Ac3H11_1817 0.62 -0.3 22,865 - Ac3H11_1817 0.62 -1.1 22,865 - Ac3H11_1817 0.62 -0.7 22,912 + Ac3H11_1817 0.67 +0.0 23,029 + Ac3H11_1817 0.79 +0.0 23,029 + Ac3H11_1817 0.79 +0.8 23,029 + Ac3H11_1817 0.79 +0.9 23,029 + Ac3H11_1817 0.79 -0.8 23,029 + Ac3H11_1817 0.79 +0.7 23,029 + Ac3H11_1817 0.79 +0.1 23,029 + Ac3H11_1817 0.79 +2.7 23,029 + Ac3H11_1817 0.79 +1.2 23,029 + Ac3H11_1817 0.79 +0.1 23,029 + Ac3H11_1817 0.79 +1.1 23,029 + Ac3H11_1817 0.79 -0.6 23,030 - Ac3H11_1817 0.79 -1.0 23,030 - Ac3H11_1817 0.79 -1.8 23,030 - Ac3H11_1817 0.79 -1.0 23,030 - Ac3H11_1817 0.79 -0.7 23,030 - Ac3H11_1817 0.79 -1.3 23,030 - Ac3H11_1817 0.79 +0.2 23,030 - Ac3H11_1817 0.79 +0.9 23,219 + +0.8 23,219 + -1.0 23,220 - -1.2 23,973 + Ac3H11_1819 0.38 +1.7 23,974 - Ac3H11_1819 0.38 -1.0
Or see this region's nucleotide sequence