Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_163 and Ac3H11_164 are separated by 89 nucleotides Ac3H11_164 and Ac3H11_165 are separated by 18 nucleotides Ac3H11_165 and Ac3H11_166 are separated by 86 nucleotides Ac3H11_166 and Ac3H11_167 are separated by 144 nucleotides
Ac3H11_163 - TRAP-type C4-dicarboxylate transport system, large permease component, at 49,281 to 50,558
_163
Ac3H11_164 - hypothetical protein, at 50,648 to 50,845
_164
Ac3H11_165 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 50,864 to 52,006
_165
Ac3H11_166 - Glycine cleavage system H protein, at 52,093 to 52,467
_166
Ac3H11_167 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 52,612 to 55,524
_167
Position (kb)
50
51
52
53 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 49.965 kb on + strand, within Ac3H11_163 at 49.966 kb on - strand, within Ac3H11_163 at 49.966 kb on - strand, within Ac3H11_163 at 49.966 kb on - strand, within Ac3H11_163 at 50.013 kb on + strand, within Ac3H11_163 at 50.013 kb on + strand, within Ac3H11_163 at 50.014 kb on - strand, within Ac3H11_163 at 50.014 kb on - strand, within Ac3H11_163 at 50.040 kb on + strand, within Ac3H11_163 at 50.040 kb on + strand, within Ac3H11_163 at 50.067 kb on + strand, within Ac3H11_163 at 50.067 kb on + strand, within Ac3H11_163 at 50.067 kb on + strand, within Ac3H11_163 at 50.067 kb on + strand, within Ac3H11_163 at 50.067 kb on + strand, within Ac3H11_163 at 50.068 kb on - strand, within Ac3H11_163 at 50.488 kb on + strand at 50.489 kb on - strand at 50.489 kb on - strand at 50.508 kb on + strand at 50.508 kb on + strand at 50.508 kb on + strand at 50.508 kb on + strand at 50.508 kb on + strand at 50.509 kb on - strand at 50.509 kb on - strand at 50.509 kb on - strand at 50.509 kb on - strand at 50.548 kb on - strand at 50.556 kb on + strand at 50.557 kb on - strand at 50.557 kb on - strand at 50.563 kb on + strand at 50.564 kb on - strand at 50.792 kb on + strand, within Ac3H11_164 at 50.793 kb on - strand, within Ac3H11_164 at 50.966 kb on + strand at 50.967 kb on - strand at 51.264 kb on + strand, within Ac3H11_165 at 51.264 kb on + strand, within Ac3H11_165 at 51.264 kb on + strand, within Ac3H11_165 at 51.264 kb on + strand, within Ac3H11_165 at 51.264 kb on + strand, within Ac3H11_165 at 51.264 kb on + strand, within Ac3H11_165 at 51.265 kb on - strand, within Ac3H11_165 at 51.265 kb on - strand, within Ac3H11_165 at 51.450 kb on - strand, within Ac3H11_165 at 51.593 kb on + strand, within Ac3H11_165 at 51.853 kb on - strand, within Ac3H11_165 at 51.887 kb on + strand, within Ac3H11_165 at 51.887 kb on + strand, within Ac3H11_165 at 51.887 kb on + strand, within Ac3H11_165 at 51.887 kb on + strand, within Ac3H11_165 at 51.888 kb on - strand, within Ac3H11_165 at 52.046 kb on + strand at 52.047 kb on - strand at 52.047 kb on - strand at 52.047 kb on - strand at 52.047 kb on - strand at 52.063 kb on + strand at 52.064 kb on - strand at 52.068 kb on - strand at 52.068 kb on - strand at 52.288 kb on + strand, within Ac3H11_166 at 52.288 kb on + strand, within Ac3H11_166 at 52.288 kb on + strand, within Ac3H11_166 at 52.288 kb on + strand, within Ac3H11_166 at 52.288 kb on + strand, within Ac3H11_166 at 52.288 kb on + strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.289 kb on - strand, within Ac3H11_166 at 52.441 kb on + strand at 52.442 kb on - strand at 52.506 kb on + strand at 52.593 kb on - strand at 52.897 kb on + strand at 52.897 kb on + strand at 52.898 kb on - strand at 52.898 kb on - strand at 52.955 kb on - strand, within Ac3H11_167 at 52.963 kb on + strand, within Ac3H11_167 at 52.963 kb on + strand, within Ac3H11_167 at 52.964 kb on - strand, within Ac3H11_167 at 52.964 kb on - strand, within Ac3H11_167
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 49,965 + Ac3H11_163 0.54 -1.2 49,966 - Ac3H11_163 0.54 +0.6 49,966 - Ac3H11_163 0.54 +0.0 49,966 - Ac3H11_163 0.54 -1.0 50,013 + Ac3H11_163 0.57 -0.5 50,013 + Ac3H11_163 0.57 -0.4 50,014 - Ac3H11_163 0.57 -1.4 50,014 - Ac3H11_163 0.57 +0.8 50,040 + Ac3H11_163 0.59 -0.1 50,040 + Ac3H11_163 0.59 +0.8 50,067 + Ac3H11_163 0.62 +0.7 50,067 + Ac3H11_163 0.62 -3.0 50,067 + Ac3H11_163 0.62 +0.2 50,067 + Ac3H11_163 0.62 +6.5 50,067 + Ac3H11_163 0.62 +0.3 50,068 - Ac3H11_163 0.62 -0.7 50,488 + -1.0 50,489 - +1.0 50,489 - +0.4 50,508 + -0.6 50,508 + +1.2 50,508 + -0.7 50,508 + +0.0 50,508 + +0.7 50,509 - +0.5 50,509 - +1.3 50,509 - +1.0 50,509 - +0.4 50,548 - +0.4 50,556 + -0.2 50,557 - -1.5 50,557 - +0.4 50,563 + -1.2 50,564 - -1.5 50,792 + Ac3H11_164 0.73 -1.4 50,793 - Ac3H11_164 0.73 +0.4 50,966 + +2.0 50,967 - +0.6 51,264 + Ac3H11_165 0.35 +0.7 51,264 + Ac3H11_165 0.35 -0.7 51,264 + Ac3H11_165 0.35 +0.3 51,264 + Ac3H11_165 0.35 -0.0 51,264 + Ac3H11_165 0.35 +0.4 51,264 + Ac3H11_165 0.35 -1.2 51,265 - Ac3H11_165 0.35 -0.2 51,265 - Ac3H11_165 0.35 -0.4 51,450 - Ac3H11_165 0.51 -1.1 51,593 + Ac3H11_165 0.64 +0.1 51,853 - Ac3H11_165 0.87 -0.7 51,887 + Ac3H11_165 0.90 -0.4 51,887 + Ac3H11_165 0.90 -0.5 51,887 + Ac3H11_165 0.90 -1.3 51,887 + Ac3H11_165 0.90 -1.0 51,888 - Ac3H11_165 0.90 +0.4 52,046 + +0.9 52,047 - +1.8 52,047 - -1.8 52,047 - +0.1 52,047 - -2.2 52,063 + +1.0 52,064 - -1.4 52,068 - +0.4 52,068 - -0.2 52,288 + Ac3H11_166 0.52 -1.6 52,288 + Ac3H11_166 0.52 -1.0 52,288 + Ac3H11_166 0.52 -0.2 52,288 + Ac3H11_166 0.52 -0.0 52,288 + Ac3H11_166 0.52 +0.2 52,288 + Ac3H11_166 0.52 +0.3 52,289 - Ac3H11_166 0.52 +0.4 52,289 - Ac3H11_166 0.52 -1.5 52,289 - Ac3H11_166 0.52 -1.7 52,289 - Ac3H11_166 0.52 -0.3 52,289 - Ac3H11_166 0.52 +3.4 52,289 - Ac3H11_166 0.52 -2.0 52,441 + -0.4 52,442 - -1.5 52,506 + -0.7 52,593 - +0.7 52,897 + +0.1 52,897 + -1.7 52,898 - +0.5 52,898 - +0.3 52,955 - Ac3H11_167 0.12 +1.8 52,963 + Ac3H11_167 0.12 +1.1 52,963 + Ac3H11_167 0.12 -0.2 52,964 - Ac3H11_167 0.12 -0.2 52,964 - Ac3H11_167 0.12 +0.6
Or see this region's nucleotide sequence