Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_140 and Ac3H11_141 are separated by 188 nucleotides Ac3H11_141 and Ac3H11_142 are separated by 73 nucleotides
Ac3H11_140 - Tricarboxylate transport protein TctC, at 23,959 to 24,915
_140
Ac3H11_141 - Tricarboxylate transport protein TctC, at 25,104 to 26,102
_141
Ac3H11_142 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 26,176 to 27,117
_142
Position (kb) 25
26
27 Strain fitness (log2 ratio) -1
0
1
2
3 at 24.185 kb on + strand, within Ac3H11_140 at 24.185 kb on + strand, within Ac3H11_140 at 24.185 kb on + strand, within Ac3H11_140 at 24.381 kb on - strand, within Ac3H11_140 at 24.654 kb on - strand, within Ac3H11_140 at 24.654 kb on - strand, within Ac3H11_140 at 24.654 kb on - strand, within Ac3H11_140 at 24.665 kb on + strand, within Ac3H11_140 at 24.666 kb on - strand, within Ac3H11_140 at 24.666 kb on - strand, within Ac3H11_140 at 24.666 kb on - strand, within Ac3H11_140 at 24.816 kb on - strand, within Ac3H11_140 at 24.926 kb on + strand at 24.926 kb on + strand at 24.926 kb on + strand at 24.926 kb on + strand at 24.927 kb on - strand at 24.927 kb on - strand at 24.927 kb on - strand at 24.931 kb on - strand at 24.956 kb on + strand at 24.957 kb on - strand at 24.957 kb on - strand at 24.957 kb on - strand at 25.142 kb on + strand at 25.142 kb on + strand at 25.142 kb on + strand at 25.142 kb on + strand at 25.143 kb on - strand at 25.144 kb on + strand at 25.145 kb on - strand at 25.321 kb on + strand, within Ac3H11_141 at 25.322 kb on - strand, within Ac3H11_141 at 25.322 kb on - strand, within Ac3H11_141 at 25.339 kb on + strand, within Ac3H11_141 at 25.339 kb on + strand, within Ac3H11_141 at 25.525 kb on + strand, within Ac3H11_141 at 25.526 kb on - strand, within Ac3H11_141 at 25.651 kb on + strand, within Ac3H11_141 at 25.651 kb on + strand, within Ac3H11_141 at 25.651 kb on + strand, within Ac3H11_141 at 25.651 kb on + strand, within Ac3H11_141 at 25.652 kb on - strand, within Ac3H11_141 at 25.652 kb on - strand, within Ac3H11_141 at 25.652 kb on - strand, within Ac3H11_141 at 25.652 kb on - strand, within Ac3H11_141 at 25.652 kb on - strand, within Ac3H11_141 at 25.713 kb on + strand, within Ac3H11_141 at 25.713 kb on + strand, within Ac3H11_141 at 25.714 kb on - strand, within Ac3H11_141 at 25.769 kb on - strand, within Ac3H11_141 at 26.043 kb on - strand at 26.047 kb on - strand at 26.259 kb on - strand at 26.576 kb on + strand, within Ac3H11_142 at 26.576 kb on + strand, within Ac3H11_142 at 26.576 kb on + strand, within Ac3H11_142 at 26.577 kb on - strand, within Ac3H11_142 at 26.577 kb on - strand, within Ac3H11_142 at 26.577 kb on - strand, within Ac3H11_142 at 26.577 kb on - strand, within Ac3H11_142 at 27.039 kb on - strand at 27.050 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 24,185 + Ac3H11_140 0.24 -1.0 24,185 + Ac3H11_140 0.24 -0.6 24,185 + Ac3H11_140 0.24 -1.5 24,381 - Ac3H11_140 0.44 -0.7 24,654 - Ac3H11_140 0.73 +0.4 24,654 - Ac3H11_140 0.73 -0.4 24,654 - Ac3H11_140 0.73 -0.9 24,665 + Ac3H11_140 0.74 -1.2 24,666 - Ac3H11_140 0.74 -1.2 24,666 - Ac3H11_140 0.74 +0.1 24,666 - Ac3H11_140 0.74 -0.8 24,816 - Ac3H11_140 0.90 +0.4 24,926 + +0.6 24,926 + +0.5 24,926 + +0.6 24,926 + +0.4 24,927 - +1.2 24,927 - +2.0 24,927 - -0.6 24,931 - -1.7 24,956 + -0.6 24,957 - +0.1 24,957 - +0.1 24,957 - +0.4 25,142 + -0.7 25,142 + +0.1 25,142 + -0.2 25,142 + -1.2 25,143 - -0.9 25,144 + -1.6 25,145 - +1.0 25,321 + Ac3H11_141 0.22 +0.9 25,322 - Ac3H11_141 0.22 +0.5 25,322 - Ac3H11_141 0.22 -0.8 25,339 + Ac3H11_141 0.24 -0.6 25,339 + Ac3H11_141 0.24 -0.8 25,525 + Ac3H11_141 0.42 +0.1 25,526 - Ac3H11_141 0.42 -1.0 25,651 + Ac3H11_141 0.55 +3.7 25,651 + Ac3H11_141 0.55 -0.6 25,651 + Ac3H11_141 0.55 -0.1 25,651 + Ac3H11_141 0.55 -0.3 25,652 - Ac3H11_141 0.55 +0.0 25,652 - Ac3H11_141 0.55 +1.5 25,652 - Ac3H11_141 0.55 +1.4 25,652 - Ac3H11_141 0.55 -0.2 25,652 - Ac3H11_141 0.55 -0.8 25,713 + Ac3H11_141 0.61 +0.6 25,713 + Ac3H11_141 0.61 +0.8 25,714 - Ac3H11_141 0.61 -0.2 25,769 - Ac3H11_141 0.67 +2.0 26,043 - +0.6 26,047 - +1.4 26,259 - +0.3 26,576 + Ac3H11_142 0.42 +1.4 26,576 + Ac3H11_142 0.42 -0.6 26,576 + Ac3H11_142 0.42 -1.4 26,577 - Ac3H11_142 0.43 -0.5 26,577 - Ac3H11_142 0.43 -1.6 26,577 - Ac3H11_142 0.43 +1.7 26,577 - Ac3H11_142 0.43 +0.3 27,039 - -0.2 27,050 + -0.3
Or see this region's nucleotide sequence