Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1338 and Ac3H11_1339 are separated by 30 nucleotides Ac3H11_1339 and Ac3H11_1340 overlap by 4 nucleotides Ac3H11_1340 and Ac3H11_1341 are separated by 917 nucleotides
Ac3H11_1338 - Mobile element protein, at 26,903 to 27,022
_1338
Ac3H11_1339 - Mobile element protein, at 27,053 to 27,376
_1339
Ac3H11_1340 - Mobile element protein, at 27,373 to 28,287
_1340
Ac3H11_1341 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 29,205 to 30,533
_1341
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 26.468 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.477 kb on + strand at 26.478 kb on - strand at 26.478 kb on - strand at 26.520 kb on + strand at 26.520 kb on + strand at 26.521 kb on - strand at 26.521 kb on - strand at 26.527 kb on - strand at 26.538 kb on + strand at 26.538 kb on + strand at 26.538 kb on + strand at 26.538 kb on + strand at 26.539 kb on - strand at 26.700 kb on - strand at 26.731 kb on + strand at 26.731 kb on + strand at 26.731 kb on + strand at 26.732 kb on - strand at 26.742 kb on + strand at 26.742 kb on + strand at 26.743 kb on - strand at 26.755 kb on + strand at 26.756 kb on - strand at 26.826 kb on + strand at 26.826 kb on + strand at 26.826 kb on + strand at 26.827 kb on - strand at 26.827 kb on - strand at 26.827 kb on - strand at 26.827 kb on - strand at 26.828 kb on + strand at 26.828 kb on + strand at 26.828 kb on + strand at 26.829 kb on - strand at 26.829 kb on - strand at 26.829 kb on - strand at 27.128 kb on + strand, within Ac3H11_1339 at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.382 kb on + strand at 27.383 kb on - strand at 27.383 kb on - strand at 27.383 kb on - strand at 27.383 kb on - strand at 27.383 kb on - strand at 27.383 kb on - strand at 27.406 kb on + strand at 27.406 kb on + strand at 27.406 kb on + strand at 27.407 kb on - strand at 27.407 kb on - strand at 27.454 kb on + strand at 27.455 kb on - strand at 27.568 kb on + strand, within Ac3H11_1340 at 27.568 kb on + strand, within Ac3H11_1340 at 27.569 kb on - strand, within Ac3H11_1340 at 27.569 kb on - strand, within Ac3H11_1340 at 27.580 kb on - strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.790 kb on + strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.791 kb on - strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.817 kb on + strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.818 kb on - strand, within Ac3H11_1340 at 27.841 kb on + strand, within Ac3H11_1340 at 27.841 kb on + strand, within Ac3H11_1340 at 27.841 kb on + strand, within Ac3H11_1340 at 27.841 kb on + strand, within Ac3H11_1340 at 27.842 kb on - strand, within Ac3H11_1340 at 27.842 kb on - strand, within Ac3H11_1340 at 27.842 kb on - strand, within Ac3H11_1340 at 27.922 kb on + strand, within Ac3H11_1340 at 27.922 kb on + strand, within Ac3H11_1340 at 27.922 kb on + strand, within Ac3H11_1340 at 27.923 kb on - strand, within Ac3H11_1340 at 27.923 kb on - strand, within Ac3H11_1340 at 27.923 kb on - strand, within Ac3H11_1340 at 27.923 kb on - strand, within Ac3H11_1340 at 27.923 kb on - strand, within Ac3H11_1340 at 27.979 kb on + strand, within Ac3H11_1340 at 27.979 kb on + strand, within Ac3H11_1340 at 27.979 kb on + strand, within Ac3H11_1340 at 27.980 kb on - strand, within Ac3H11_1340 at 27.980 kb on - strand, within Ac3H11_1340 at 27.980 kb on - strand, within Ac3H11_1340 at 27.980 kb on - strand, within Ac3H11_1340 at 27.980 kb on - strand, within Ac3H11_1340 at 27.994 kb on + strand, within Ac3H11_1340 at 27.994 kb on + strand, within Ac3H11_1340 at 27.995 kb on - strand, within Ac3H11_1340 at 27.995 kb on - strand, within Ac3H11_1340 at 28.022 kb on + strand, within Ac3H11_1340 at 28.022 kb on + strand at 28.022 kb on + strand, within Ac3H11_1340 at 28.022 kb on + strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.023 kb on - strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.054 kb on + strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.055 kb on - strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.090 kb on + strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.091 kb on - strand, within Ac3H11_1340 at 28.532 kb on + strand at 28.602 kb on + strand at 28.602 kb on + strand at 28.602 kb on + strand at 28.603 kb on - strand at 28.603 kb on - strand at 28.603 kb on - strand at 28.625 kb on + strand at 28.625 kb on + strand at 28.625 kb on + strand at 28.634 kb on + strand at 28.635 kb on - strand at 28.652 kb on - strand at 28.683 kb on + strand at 28.683 kb on + strand at 28.683 kb on + strand at 28.684 kb on - strand at 28.695 kb on + strand at 28.695 kb on + strand at 28.695 kb on + strand at 28.695 kb on + strand at 28.695 kb on + strand at 28.695 kb on + strand at 28.696 kb on - strand at 28.696 kb on - strand at 28.696 kb on - strand at 28.696 kb on - strand at 28.696 kb on - strand at 28.696 kb on - strand at 28.719 kb on + strand at 28.719 kb on + strand at 28.719 kb on + strand at 28.719 kb on + strand at 28.720 kb on - strand at 28.720 kb on - strand at 28.724 kb on + strand at 28.724 kb on + strand at 28.724 kb on + strand at 28.724 kb on + strand at 28.725 kb on - strand at 28.725 kb on - strand at 28.725 kb on - strand at 28.734 kb on - strand at 28.793 kb on + strand at 28.804 kb on + strand at 28.804 kb on + strand at 28.804 kb on + strand at 28.814 kb on + strand at 28.814 kb on + strand at 28.815 kb on - strand at 28.869 kb on + strand at 28.870 kb on - strand at 28.870 kb on - strand at 28.870 kb on - strand at 28.891 kb on + strand at 28.892 kb on - strand at 28.968 kb on + strand at 28.969 kb on - strand at 28.969 kb on - strand at 29.105 kb on + strand at 29.135 kb on + strand at 29.135 kb on + strand at 29.135 kb on + strand at 29.136 kb on - strand at 29.136 kb on - strand at 29.136 kb on - strand at 29.195 kb on + strand at 29.196 kb on - strand at 29.196 kb on - strand at 29.196 kb on - strand at 29.196 kb on - strand at 29.196 kb on - strand at 29.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 26,468 + +1.1 26,477 + -0.3 26,477 + -0.4 26,477 + -1.7 26,477 + -0.9 26,477 + -0.9 26,477 + -1.6 26,477 + -0.6 26,477 + +0.8 26,478 - +1.7 26,478 - +1.0 26,520 + +0.8 26,520 + -0.3 26,521 - -0.7 26,521 - +0.5 26,527 - -1.8 26,538 + +0.4 26,538 + +0.1 26,538 + +0.7 26,538 + -1.0 26,539 - -0.1 26,700 - -0.5 26,731 + +0.2 26,731 + -1.3 26,731 + -1.2 26,732 - +2.1 26,742 + +0.8 26,742 + +1.1 26,743 - -0.1 26,755 + -0.3 26,756 - -0.6 26,826 + +0.8 26,826 + +4.6 26,826 + -1.4 26,827 - -0.7 26,827 - -2.0 26,827 - -0.1 26,827 - -1.0 26,828 + -0.8 26,828 + -1.2 26,828 + +0.7 26,829 - +0.5 26,829 - +1.2 26,829 - -0.2 27,128 + Ac3H11_1339 0.23 -0.4 27,382 + +1.7 27,382 + +2.4 27,382 + -0.3 27,382 + -2.3 27,382 + -1.9 27,382 + -0.8 27,382 + +0.7 27,382 + +1.7 27,382 + -0.4 27,382 + +0.4 27,382 + +0.4 27,383 - -0.2 27,383 - -2.0 27,383 - +0.4 27,383 - +0.3 27,383 - +0.2 27,383 - -1.6 27,406 + -0.1 27,406 + +0.6 27,406 + -0.2 27,407 - +0.8 27,407 - +0.8 27,454 + -1.1 27,455 - +0.3 27,568 + Ac3H11_1340 0.21 -0.3 27,568 + Ac3H11_1340 0.21 +0.0 27,569 - Ac3H11_1340 0.21 -3.4 27,569 - Ac3H11_1340 0.21 -0.9 27,580 - Ac3H11_1340 0.23 -2.0 27,790 + Ac3H11_1340 0.46 +0.3 27,790 + Ac3H11_1340 0.46 -1.4 27,790 + Ac3H11_1340 0.46 -0.8 27,790 + Ac3H11_1340 0.46 +2.1 27,790 + Ac3H11_1340 0.46 +0.4 27,790 + Ac3H11_1340 0.46 +2.9 27,790 + Ac3H11_1340 0.46 -1.8 27,790 + Ac3H11_1340 0.46 -2.8 27,791 - Ac3H11_1340 0.46 -0.4 27,791 - Ac3H11_1340 0.46 +1.0 27,791 - Ac3H11_1340 0.46 -1.5 27,791 - Ac3H11_1340 0.46 -0.6 27,791 - Ac3H11_1340 0.46 -0.2 27,791 - Ac3H11_1340 0.46 +0.5 27,791 - Ac3H11_1340 0.46 +0.4 27,791 - Ac3H11_1340 0.46 +0.2 27,791 - Ac3H11_1340 0.46 +2.0 27,791 - Ac3H11_1340 0.46 -1.7 27,791 - Ac3H11_1340 0.46 +0.3 27,817 + Ac3H11_1340 0.49 +0.3 27,817 + Ac3H11_1340 0.49 -0.5 27,817 + Ac3H11_1340 0.49 +1.2 27,817 + Ac3H11_1340 0.49 -0.1 27,817 + Ac3H11_1340 0.49 -0.1 27,817 + Ac3H11_1340 0.49 +1.4 27,817 + Ac3H11_1340 0.49 +0.7 27,817 + Ac3H11_1340 0.49 +1.0 27,818 - Ac3H11_1340 0.49 -0.1 27,818 - Ac3H11_1340 0.49 +0.1 27,818 - Ac3H11_1340 0.49 -0.0 27,818 - Ac3H11_1340 0.49 -0.9 27,818 - Ac3H11_1340 0.49 +0.6 27,818 - Ac3H11_1340 0.49 +1.7 27,841 + Ac3H11_1340 0.51 -0.2 27,841 + Ac3H11_1340 0.51 +0.0 27,841 + Ac3H11_1340 0.51 -0.9 27,841 + Ac3H11_1340 0.51 -1.0 27,842 - Ac3H11_1340 0.51 -0.6 27,842 - Ac3H11_1340 0.51 +0.2 27,842 - Ac3H11_1340 0.51 -0.2 27,922 + Ac3H11_1340 0.60 +1.0 27,922 + Ac3H11_1340 0.60 -0.1 27,922 + Ac3H11_1340 0.60 -0.0 27,923 - Ac3H11_1340 0.60 +1.2 27,923 - Ac3H11_1340 0.60 +0.5 27,923 - Ac3H11_1340 0.60 -0.6 27,923 - Ac3H11_1340 0.60 +0.5 27,923 - Ac3H11_1340 0.60 -1.9 27,979 + Ac3H11_1340 0.66 +2.7 27,979 + Ac3H11_1340 0.66 +0.1 27,979 + Ac3H11_1340 0.66 -0.3 27,980 - Ac3H11_1340 0.66 -0.4 27,980 - Ac3H11_1340 0.66 +2.4 27,980 - Ac3H11_1340 0.66 +1.7 27,980 - Ac3H11_1340 0.66 +0.4 27,980 - Ac3H11_1340 0.66 -0.5 27,994 + Ac3H11_1340 0.68 -2.0 27,994 + Ac3H11_1340 0.68 -0.3 27,995 - Ac3H11_1340 0.68 -1.3 27,995 - Ac3H11_1340 0.68 +0.7 28,022 + Ac3H11_1340 0.71 -1.1 28,022 + +2.7 28,022 + Ac3H11_1340 0.71 +1.7 28,022 + Ac3H11_1340 0.71 -0.2 28,023 - Ac3H11_1340 0.71 -1.0 28,023 - Ac3H11_1340 0.71 -2.0 28,023 - Ac3H11_1340 0.71 -0.2 28,023 - Ac3H11_1340 0.71 +0.4 28,023 - Ac3H11_1340 0.71 -0.4 28,023 - Ac3H11_1340 0.71 -0.2 28,054 + Ac3H11_1340 0.74 -0.7 28,054 + Ac3H11_1340 0.74 -1.6 28,054 + Ac3H11_1340 0.74 -2.1 28,054 + Ac3H11_1340 0.74 -0.8 28,054 + Ac3H11_1340 0.74 -1.3 28,054 + Ac3H11_1340 0.74 -1.0 28,054 + Ac3H11_1340 0.74 +0.4 28,054 + Ac3H11_1340 0.74 +0.1 28,054 + Ac3H11_1340 0.74 +0.5 28,054 + Ac3H11_1340 0.74 +0.4 28,054 + Ac3H11_1340 0.74 +1.7 28,054 + Ac3H11_1340 0.74 +0.5 28,054 + Ac3H11_1340 0.74 +0.3 28,054 + Ac3H11_1340 0.74 -2.5 28,054 + Ac3H11_1340 0.74 -1.0 28,054 + Ac3H11_1340 0.74 +0.7 28,055 - Ac3H11_1340 0.75 +0.5 28,055 - Ac3H11_1340 0.75 -0.9 28,055 - Ac3H11_1340 0.75 +0.2 28,055 - Ac3H11_1340 0.75 +0.9 28,055 - Ac3H11_1340 0.75 +0.6 28,055 - Ac3H11_1340 0.75 -1.2 28,055 - Ac3H11_1340 0.75 +0.5 28,055 - Ac3H11_1340 0.75 +0.1 28,055 - Ac3H11_1340 0.75 -1.3 28,055 - Ac3H11_1340 0.75 +1.2 28,055 - Ac3H11_1340 0.75 -1.5 28,055 - Ac3H11_1340 0.75 +1.0 28,055 - Ac3H11_1340 0.75 -0.6 28,055 - Ac3H11_1340 0.75 -0.8 28,055 - Ac3H11_1340 0.75 -1.5 28,055 - Ac3H11_1340 0.75 +0.7 28,055 - Ac3H11_1340 0.75 +1.4 28,090 + Ac3H11_1340 0.78 +0.7 28,090 + Ac3H11_1340 0.78 +1.2 28,090 + Ac3H11_1340 0.78 +0.1 28,090 + Ac3H11_1340 0.78 +0.2 28,090 + Ac3H11_1340 0.78 -0.0 28,090 + Ac3H11_1340 0.78 -0.7 28,090 + Ac3H11_1340 0.78 -0.0 28,090 + Ac3H11_1340 0.78 +0.1 28,090 + Ac3H11_1340 0.78 +0.0 28,090 + Ac3H11_1340 0.78 -3.0 28,090 + Ac3H11_1340 0.78 -2.7 28,090 + Ac3H11_1340 0.78 +1.9 28,091 - Ac3H11_1340 0.78 +1.7 28,091 - Ac3H11_1340 0.78 -0.3 28,091 - Ac3H11_1340 0.78 -1.3 28,091 - Ac3H11_1340 0.78 -2.1 28,091 - Ac3H11_1340 0.78 -1.6 28,091 - Ac3H11_1340 0.78 -1.0 28,091 - Ac3H11_1340 0.78 -0.2 28,091 - Ac3H11_1340 0.78 -1.4 28,532 + -1.8 28,602 + +1.1 28,602 + -1.3 28,602 + +0.5 28,603 - -0.3 28,603 - +2.7 28,603 - -2.1 28,625 + -0.1 28,625 + +0.3 28,625 + -0.1 28,634 + +0.5 28,635 - +0.9 28,652 - +1.0 28,683 + -0.9 28,683 + -0.6 28,683 + -1.1 28,684 - +1.7 28,695 + +0.6 28,695 + +0.5 28,695 + -2.0 28,695 + +1.7 28,695 + -0.6 28,695 + +0.5 28,696 - +1.7 28,696 - -0.2 28,696 - +4.6 28,696 - +0.3 28,696 - -1.4 28,696 - +0.8 28,719 + -0.5 28,719 + +0.7 28,719 + -1.2 28,719 + +0.3 28,720 - +2.7 28,720 - +1.7 28,724 + -0.8 28,724 + -1.1 28,724 + -0.1 28,724 + +1.2 28,725 - -0.3 28,725 - +0.1 28,725 - +0.5 28,734 - +0.7 28,793 + +1.7 28,804 + -0.1 28,804 + -0.9 28,804 + +2.0 28,814 + -1.1 28,814 + +0.2 28,815 - -0.6 28,869 + +0.3 28,870 - +0.3 28,870 - -1.1 28,870 - -1.8 28,891 + -0.0 28,892 - +0.1 28,968 + -0.4 28,969 - -0.1 28,969 - -0.9 29,105 + +0.2 29,135 + -1.1 29,135 + +1.5 29,135 + -0.1 29,136 - +2.1 29,136 - +1.6 29,136 - +0.2 29,195 + +1.9 29,196 - -0.6 29,196 - -0.0 29,196 - -0.5 29,196 - +0.2 29,196 - +1.7 29,206 + -1.1
Or see this region's nucleotide sequence