Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1181 and Ac3H11_1182 are separated by 188 nucleotides Ac3H11_1182 and Ac3H11_1183 are separated by 35 nucleotides Ac3H11_1183 and Ac3H11_1184 are separated by 136 nucleotides Ac3H11_1184 and Ac3H11_1185 are separated by 88 nucleotides
Ac3H11_1181 - Transcriptional regulator ligR, LysR family, at 18,836 to 19,786
_1181
Ac3H11_1182 - Hypothetical ABC transport system, periplasmic component, at 19,975 to 20,676
_1182
Ac3H11_1183 - 4-oxalomesaconate hydratase (EC 4.2.1.83), at 20,712 to 21,746
_1183
Ac3H11_1184 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase (EC 4.1.3.17), at 21,883 to 22,566
_1184
Ac3H11_1185 - putative exported protein, at 22,655 to 23,665
_1185
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 19.764 kb on + strand at 19.764 kb on + strand at 19.804 kb on - strand at 19.804 kb on - strand at 19.840 kb on + strand at 19.840 kb on + strand at 19.842 kb on + strand at 19.842 kb on + strand at 19.842 kb on + strand at 19.843 kb on - strand at 19.850 kb on - strand at 20.050 kb on + strand, within Ac3H11_1182 at 20.050 kb on + strand, within Ac3H11_1182 at 20.617 kb on + strand at 20.742 kb on + strand at 20.742 kb on + strand at 20.743 kb on - strand at 20.743 kb on - strand at 20.743 kb on - strand at 21.120 kb on + strand, within Ac3H11_1183 at 21.210 kb on + strand, within Ac3H11_1183 at 21.211 kb on - strand, within Ac3H11_1183 at 21.228 kb on + strand, within Ac3H11_1183 at 21.405 kb on + strand, within Ac3H11_1183 at 21.406 kb on - strand, within Ac3H11_1183 at 21.501 kb on + strand, within Ac3H11_1183 at 21.612 kb on + strand, within Ac3H11_1183 at 21.613 kb on - strand, within Ac3H11_1183 at 21.613 kb on - strand, within Ac3H11_1183 at 21.613 kb on - strand, within Ac3H11_1183 at 21.616 kb on - strand, within Ac3H11_1183 at 21.634 kb on - strand, within Ac3H11_1183 at 21.634 kb on - strand, within Ac3H11_1183 at 21.634 kb on - strand, within Ac3H11_1183 at 21.681 kb on + strand at 21.682 kb on - strand at 21.682 kb on - strand at 21.886 kb on + strand at 21.887 kb on - strand at 21.887 kb on - strand at 21.887 kb on - strand at 21.893 kb on + strand at 21.893 kb on + strand at 21.894 kb on - strand at 22.165 kb on + strand, within Ac3H11_1184 at 22.166 kb on - strand, within Ac3H11_1184 at 22.516 kb on + strand at 22.516 kb on + strand at 22.516 kb on + strand at 22.516 kb on + strand at 22.517 kb on - strand at 22.517 kb on - strand at 22.517 kb on - strand at 22.517 kb on - strand at 22.517 kb on - strand at 22.517 kb on - strand at 22.608 kb on + strand at 22.609 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 19,764 + +0.3 19,764 + +0.0 19,804 - +0.0 19,804 - +1.1 19,840 + -1.2 19,840 + +0.4 19,842 + -2.2 19,842 + +3.4 19,842 + -1.1 19,843 - -0.7 19,850 - -0.7 20,050 + Ac3H11_1182 0.11 -2.7 20,050 + Ac3H11_1182 0.11 +0.5 20,617 + -1.0 20,742 + -0.3 20,742 + +3.3 20,743 - +2.0 20,743 - -0.7 20,743 - -1.3 21,120 + Ac3H11_1183 0.39 +0.4 21,210 + Ac3H11_1183 0.48 +0.7 21,211 - Ac3H11_1183 0.48 -1.2 21,228 + Ac3H11_1183 0.50 -0.9 21,405 + Ac3H11_1183 0.67 -1.9 21,406 - Ac3H11_1183 0.67 -1.2 21,501 + Ac3H11_1183 0.76 +1.1 21,612 + Ac3H11_1183 0.87 -0.1 21,613 - Ac3H11_1183 0.87 +0.0 21,613 - Ac3H11_1183 0.87 +0.6 21,613 - Ac3H11_1183 0.87 +0.6 21,616 - Ac3H11_1183 0.87 +0.3 21,634 - Ac3H11_1183 0.89 -0.3 21,634 - Ac3H11_1183 0.89 +0.6 21,634 - Ac3H11_1183 0.89 +0.4 21,681 + +0.6 21,682 - -0.7 21,682 - -1.1 21,886 + -0.1 21,887 - +0.4 21,887 - -0.3 21,887 - -2.8 21,893 + -0.5 21,893 + -0.9 21,894 - +0.4 22,165 + Ac3H11_1184 0.41 +0.8 22,166 - Ac3H11_1184 0.41 -0.9 22,516 + +1.1 22,516 + +3.5 22,516 + +0.5 22,516 + -0.6 22,517 - -1.5 22,517 - +1.5 22,517 - +0.1 22,517 - +0.6 22,517 - +1.1 22,517 - +1.1 22,608 + -0.7 22,609 - +0.8
Or see this region's nucleotide sequence