Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_842 and Pf1N1B4_843 are separated by 100 nucleotides Pf1N1B4_843 and Pf1N1B4_844 are separated by 59 nucleotides
Pf1N1B4_842 - putative membrane protein, at 970,424 to 971,191
_842
Pf1N1B4_843 - Transcriptional regulator, LysR family, at 971,292 to 972,242
_843
Pf1N1B4_844 - Exodeoxyribonuclease VII large subunit (EC 3.1.11.6), at 972,302 to 973,708
_844
Position (kb)
971
972
973 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 970.329 kb on + strand at 970.329 kb on + strand at 970.330 kb on - strand at 970.330 kb on - strand at 970.405 kb on - strand at 970.405 kb on - strand at 970.437 kb on + strand at 970.438 kb on - strand at 970.438 kb on - strand at 970.599 kb on + strand, within Pf1N1B4_842 at 970.599 kb on + strand at 970.600 kb on - strand, within Pf1N1B4_842 at 970.704 kb on + strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.713 kb on + strand, within Pf1N1B4_842 at 970.714 kb on - strand, within Pf1N1B4_842 at 970.715 kb on + strand, within Pf1N1B4_842 at 970.716 kb on - strand, within Pf1N1B4_842 at 970.811 kb on - strand, within Pf1N1B4_842 at 970.811 kb on - strand, within Pf1N1B4_842 at 970.824 kb on + strand, within Pf1N1B4_842 at 971.102 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.174 kb on + strand at 971.175 kb on - strand at 971.175 kb on - strand at 971.208 kb on + strand at 971.209 kb on - strand at 971.248 kb on + strand at 971.251 kb on + strand at 971.251 kb on + strand at 971.252 kb on - strand at 971.367 kb on + strand at 971.372 kb on + strand at 971.540 kb on + strand, within Pf1N1B4_843 at 971.541 kb on - strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.634 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.923 kb on - strand, within Pf1N1B4_843 at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.162 kb on - strand at 972.162 kb on - strand at 972.167 kb on + strand at 972.167 kb on + strand at 972.168 kb on - strand at 972.168 kb on - strand at 972.168 kb on - strand at 972.227 kb on + strand at 972.228 kb on - strand at 972.228 kb on - strand at 972.228 kb on - strand at 972.241 kb on - strand at 972.316 kb on + strand at 972.317 kb on - strand at 972.334 kb on + strand at 972.453 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.630 kb on - strand, within Pf1N1B4_844 at 973.237 kb on + strand, within Pf1N1B4_844 at 973.238 kb on - strand, within Pf1N1B4_844 at 973.238 kb on - strand, within Pf1N1B4_844
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 970,329 + -0.8 970,329 + +2.5 970,330 - -1.8 970,330 - +0.2 970,405 - +1.1 970,405 - +1.9 970,437 + +2.2 970,438 - -0.6 970,438 - -0.8 970,599 + Pf1N1B4_842 0.23 -0.7 970,599 + +0.2 970,600 - Pf1N1B4_842 0.23 +2.3 970,704 + Pf1N1B4_842 0.36 +1.0 970,705 - Pf1N1B4_842 0.37 -1.0 970,705 - Pf1N1B4_842 0.37 +0.9 970,705 - Pf1N1B4_842 0.37 -2.7 970,705 - Pf1N1B4_842 0.37 -0.8 970,705 - Pf1N1B4_842 0.37 -0.1 970,713 + Pf1N1B4_842 0.38 -2.4 970,714 - Pf1N1B4_842 0.38 +1.5 970,715 + Pf1N1B4_842 0.38 +0.2 970,716 - Pf1N1B4_842 0.38 +1.2 970,811 - Pf1N1B4_842 0.50 +0.2 970,811 - Pf1N1B4_842 0.50 -0.8 970,824 + Pf1N1B4_842 0.52 +0.5 971,102 + Pf1N1B4_842 0.88 -0.1 971,109 + Pf1N1B4_842 0.89 -0.4 971,109 + Pf1N1B4_842 0.89 -3.2 971,109 + Pf1N1B4_842 0.89 +0.9 971,109 + +1.5 971,110 - Pf1N1B4_842 0.89 +1.2 971,110 - Pf1N1B4_842 0.89 -0.8 971,110 - Pf1N1B4_842 0.89 -1.3 971,110 - Pf1N1B4_842 0.89 -1.2 971,110 - Pf1N1B4_842 0.89 -1.4 971,174 + -0.4 971,175 - -0.2 971,175 - -0.6 971,208 + +1.8 971,209 - -1.8 971,248 + -0.4 971,251 + +1.2 971,251 + -0.8 971,252 - -0.7 971,367 + +1.2 971,372 + -0.8 971,540 + Pf1N1B4_843 0.26 -2.4 971,541 - Pf1N1B4_843 0.26 -2.2 971,600 + Pf1N1B4_843 0.32 -1.4 971,600 + Pf1N1B4_843 0.32 -0.8 971,601 - Pf1N1B4_843 0.32 -1.8 971,601 - Pf1N1B4_843 0.32 -1.8 971,634 + Pf1N1B4_843 0.36 -0.4 971,784 + Pf1N1B4_843 0.52 +0.3 971,784 + Pf1N1B4_843 0.52 -0.4 971,784 + Pf1N1B4_843 0.52 -1.4 971,816 + Pf1N1B4_843 0.55 -1.8 971,816 + +1.2 971,922 + Pf1N1B4_843 0.66 -0.8 971,922 + Pf1N1B4_843 0.66 +0.2 971,922 + Pf1N1B4_843 0.66 -2.2 971,922 + Pf1N1B4_843 0.66 +1.7 971,923 - Pf1N1B4_843 0.66 -2.8 972,161 + -1.4 972,161 + -0.8 972,161 + +0.7 972,161 + -0.3 972,162 - +1.3 972,162 - -0.8 972,167 + +0.5 972,167 + -0.3 972,168 - -2.2 972,168 - -0.2 972,168 - -2.8 972,227 + +1.1 972,228 - -1.2 972,228 - -0.2 972,228 - -0.8 972,241 - +0.6 972,316 + -1.4 972,317 - -2.4 972,334 + -3.0 972,453 + Pf1N1B4_844 0.11 +0.3 972,488 + Pf1N1B4_844 0.13 -2.2 972,488 + Pf1N1B4_844 0.13 +1.7 972,488 + Pf1N1B4_844 0.13 -0.8 972,488 + Pf1N1B4_844 0.13 +1.5 972,488 + Pf1N1B4_844 0.13 -1.8 972,488 + Pf1N1B4_844 0.13 -1.8 972,489 - Pf1N1B4_844 0.13 +0.2 972,489 - Pf1N1B4_844 0.13 -0.3 972,489 - Pf1N1B4_844 0.13 +0.4 972,489 - Pf1N1B4_844 0.13 -0.3 972,489 - Pf1N1B4_844 0.13 +0.6 972,489 - Pf1N1B4_844 0.13 -0.8 972,489 - Pf1N1B4_844 0.13 +0.4 972,630 - Pf1N1B4_844 0.23 -0.3 973,237 + Pf1N1B4_844 0.66 -0.8 973,238 - Pf1N1B4_844 0.67 -2.2 973,238 - Pf1N1B4_844 0.67 +0.2
Or see this region's nucleotide sequence