Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_812 and Pf1N1B4_813 are separated by 462 nucleotides Pf1N1B4_813 and Pf1N1B4_814 are separated by 514 nucleotides
Pf1N1B4_812 - CFA/I fimbrial subunit C precursor, at 928,651 to 930,819
_812
Pf1N1B4_813 - hypothetical protein, at 931,282 to 931,776
_813
Pf1N1B4_814 - Transcriptional regulator, PadR family, at 932,291 to 932,872
_814
Position (kb)
931
932 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 930.350 kb on + strand at 930.350 kb on + strand, within Pf1N1B4_812 at 930.350 kb on + strand, within Pf1N1B4_812 at 930.351 kb on - strand, within Pf1N1B4_812 at 930.351 kb on - strand, within Pf1N1B4_812 at 930.351 kb on - strand, within Pf1N1B4_812 at 930.351 kb on - strand, within Pf1N1B4_812 at 930.541 kb on + strand, within Pf1N1B4_812 at 930.541 kb on + strand, within Pf1N1B4_812 at 930.541 kb on + strand, within Pf1N1B4_812 at 930.541 kb on + strand, within Pf1N1B4_812 at 930.541 kb on + strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.542 kb on - strand, within Pf1N1B4_812 at 930.552 kb on - strand, within Pf1N1B4_812 at 930.574 kb on + strand, within Pf1N1B4_812 at 930.732 kb on - strand at 930.732 kb on - strand at 930.732 kb on - strand at 930.735 kb on - strand at 930.735 kb on - strand at 930.872 kb on + strand at 930.872 kb on + strand at 930.964 kb on + strand at 930.965 kb on - strand at 930.965 kb on - strand at 931.249 kb on + strand at 931.249 kb on + strand at 931.250 kb on - strand at 931.257 kb on + strand at 931.271 kb on + strand at 931.272 kb on - strand at 931.337 kb on + strand, within Pf1N1B4_813 at 931.337 kb on + strand, within Pf1N1B4_813 at 931.391 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.434 kb on + strand, within Pf1N1B4_813 at 931.435 kb on - strand, within Pf1N1B4_813 at 931.444 kb on + strand, within Pf1N1B4_813 at 931.444 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand, within Pf1N1B4_813 at 931.479 kb on + strand at 931.479 kb on + strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.480 kb on - strand, within Pf1N1B4_813 at 931.494 kb on - strand, within Pf1N1B4_813 at 931.494 kb on - strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.546 kb on + strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.547 kb on - strand, within Pf1N1B4_813 at 931.556 kb on + strand, within Pf1N1B4_813 at 931.557 kb on - strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.646 kb on + strand, within Pf1N1B4_813 at 931.647 kb on - strand, within Pf1N1B4_813 at 931.778 kb on + strand at 931.779 kb on - strand at 931.781 kb on + strand at 931.797 kb on + strand at 931.797 kb on + strand at 932.108 kb on + strand at 932.146 kb on + strand at 932.148 kb on + strand at 932.148 kb on + strand at 932.148 kb on + strand at 932.251 kb on + strand at 932.267 kb on + strand at 932.460 kb on - strand, within Pf1N1B4_814 at 932.502 kb on - strand, within Pf1N1B4_814 at 932.525 kb on + strand, within Pf1N1B4_814 at 932.626 kb on - strand, within Pf1N1B4_814
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 930,350 + -0.9 930,350 + Pf1N1B4_812 0.78 +0.1 930,350 + Pf1N1B4_812 0.78 +2.7 930,351 - Pf1N1B4_812 0.78 -1.5 930,351 - Pf1N1B4_812 0.78 -1.5 930,351 - Pf1N1B4_812 0.78 -1.5 930,351 - Pf1N1B4_812 0.78 -0.5 930,541 + Pf1N1B4_812 0.87 +1.4 930,541 + Pf1N1B4_812 0.87 -0.2 930,541 + Pf1N1B4_812 0.87 -3.5 930,541 + Pf1N1B4_812 0.87 -2.9 930,541 + Pf1N1B4_812 0.87 +0.4 930,542 - Pf1N1B4_812 0.87 -0.9 930,542 - Pf1N1B4_812 0.87 -3.5 930,542 - Pf1N1B4_812 0.87 +1.1 930,542 - Pf1N1B4_812 0.87 -0.9 930,542 - Pf1N1B4_812 0.87 +0.7 930,542 - Pf1N1B4_812 0.87 -2.2 930,542 - Pf1N1B4_812 0.87 -0.9 930,542 - Pf1N1B4_812 0.87 +1.8 930,542 - Pf1N1B4_812 0.87 -1.5 930,552 - Pf1N1B4_812 0.88 -1.1 930,574 + Pf1N1B4_812 0.89 +0.1 930,732 - -1.9 930,732 - +0.4 930,732 - -0.9 930,735 - -1.5 930,735 - +2.1 930,872 + -0.9 930,872 + +0.8 930,964 + -1.9 930,965 - -0.3 930,965 - +1.7 931,249 + -0.9 931,249 + -0.9 931,250 - -1.6 931,257 + -1.5 931,271 + -1.9 931,272 - -0.9 931,337 + Pf1N1B4_813 0.11 -2.9 931,337 + Pf1N1B4_813 0.11 -1.5 931,391 + Pf1N1B4_813 0.22 -2.5 931,434 + Pf1N1B4_813 0.31 -1.5 931,434 + Pf1N1B4_813 0.31 -0.6 931,434 + Pf1N1B4_813 0.31 -1.9 931,435 - Pf1N1B4_813 0.31 -0.6 931,444 + Pf1N1B4_813 0.33 -1.9 931,444 + Pf1N1B4_813 0.33 -2.9 931,479 + Pf1N1B4_813 0.40 -3.0 931,479 + Pf1N1B4_813 0.40 -1.5 931,479 + +0.1 931,479 + Pf1N1B4_813 0.40 -1.7 931,480 - Pf1N1B4_813 0.40 -1.2 931,480 - Pf1N1B4_813 0.40 -1.0 931,480 - Pf1N1B4_813 0.40 -0.6 931,480 - Pf1N1B4_813 0.40 -0.6 931,494 - Pf1N1B4_813 0.43 -1.9 931,494 - Pf1N1B4_813 0.43 -1.5 931,546 + Pf1N1B4_813 0.53 -0.9 931,546 + Pf1N1B4_813 0.53 -1.9 931,546 + Pf1N1B4_813 0.53 -0.9 931,546 + Pf1N1B4_813 0.53 +0.1 931,546 + Pf1N1B4_813 0.53 +0.5 931,546 + Pf1N1B4_813 0.53 -0.5 931,547 - Pf1N1B4_813 0.54 -0.5 931,547 - Pf1N1B4_813 0.54 -0.9 931,547 - Pf1N1B4_813 0.54 -0.9 931,547 - Pf1N1B4_813 0.54 -1.9 931,547 - Pf1N1B4_813 0.54 +0.5 931,547 - Pf1N1B4_813 0.54 -2.5 931,547 - Pf1N1B4_813 0.54 +0.1 931,556 + Pf1N1B4_813 0.55 -0.9 931,557 - Pf1N1B4_813 0.56 -2.7 931,646 + Pf1N1B4_813 0.74 -1.5 931,646 + Pf1N1B4_813 0.74 -1.5 931,646 + Pf1N1B4_813 0.74 -1.5 931,646 + Pf1N1B4_813 0.74 -3.3 931,647 - Pf1N1B4_813 0.74 -2.0 931,778 + -3.0 931,779 - -0.9 931,781 + -1.9 931,797 + -1.5 931,797 + -3.2 932,108 + -1.5 932,146 + +1.1 932,148 + -3.0 932,148 + +0.1 932,148 + -1.5 932,251 + -1.9 932,267 + -4.3 932,460 - Pf1N1B4_814 0.29 +1.7 932,502 - Pf1N1B4_814 0.36 -0.0 932,525 + Pf1N1B4_814 0.40 -0.9 932,626 - Pf1N1B4_814 0.58 +0.1
Or see this region's nucleotide sequence