Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_770

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_769 and Pf1N1B4_770 are separated by 324 nucleotidesPf1N1B4_770 and Pf1N1B4_771 overlap by 18 nucleotides Pf1N1B4_769 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 873,701 to 875,161 _769 Pf1N1B4_770 - hypothetical protein, at 875,486 to 875,626 _770 Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component (from data), at 875,609 to 876,523 _771 Position (kb) 875 876Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.652 kb on + strand, within Pf1N1B4_769at 874.653 kb on - strand, within Pf1N1B4_769at 874.766 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.957 kb on + strand, within Pf1N1B4_769at 874.991 kb on + strand, within Pf1N1B4_769at 874.991 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.114 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.226 kb on - strandat 875.226 kb on - strandat 875.281 kb on + strandat 875.282 kb on - strandat 875.284 kb on + strandat 875.284 kb on + strandat 875.285 kb on - strandat 875.285 kb on - strandat 875.299 kb on + strandat 875.340 kb on - strandat 875.410 kb on - strandat 875.464 kb on - strandat 875.494 kb on + strandat 875.582 kb on + strand, within Pf1N1B4_770at 875.582 kb on + strand, within Pf1N1B4_770at 875.583 kb on - strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.762 kb on + strand, within Pf1N1B4_771at 875.795 kb on + strand, within Pf1N1B4_771at 875.871 kb on - strand, within Pf1N1B4_771at 875.871 kb on - strand, within Pf1N1B4_771at 876.035 kb on + strand, within Pf1N1B4_771at 876.036 kb on - strand, within Pf1N1B4_771at 876.036 kb on - strandat 876.036 kb on - strand, within Pf1N1B4_771at 876.302 kb on + strand, within Pf1N1B4_771at 876.303 kb on - strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.371 kb on + strand, within Pf1N1B4_771at 876.372 kb on - strand, within Pf1N1B4_771at 876.372 kb on - strand, within Pf1N1B4_771at 876.372 kb on - strand, within Pf1N1B4_771

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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874,589 + Pf1N1B4_769 0.61 +2.2
874,589 + Pf1N1B4_769 0.61 +0.8
874,589 + Pf1N1B4_769 0.61 +2.5
874,589 + Pf1N1B4_769 0.61 +0.7
874,652 + Pf1N1B4_769 0.65 +4.5
874,653 - Pf1N1B4_769 0.65 +1.1
874,766 + Pf1N1B4_769 0.73 +1.1
874,913 + Pf1N1B4_769 0.83 +1.7
874,913 + Pf1N1B4_769 0.83 +0.3
874,913 + Pf1N1B4_769 0.83 +1.9
874,913 + Pf1N1B4_769 0.83 +2.1
874,957 + Pf1N1B4_769 0.86 -0.0
874,991 + Pf1N1B4_769 0.88 +1.1
874,991 + Pf1N1B4_769 0.88 +2.8
875,009 + Pf1N1B4_769 0.90 +1.8
875,009 + Pf1N1B4_769 0.90 +3.4
875,009 + Pf1N1B4_769 0.90 +1.1
875,114 + +1.3
875,225 + +1.3
875,225 + +0.1
875,225 + -0.4
875,225 + -1.5
875,226 - -1.9
875,226 - -0.9
875,281 + +1.7
875,282 - +0.1
875,284 + -0.2
875,284 + +0.3
875,285 - -2.2
875,285 - -0.7
875,299 + +0.3
875,340 - +1.7
875,410 - +1.1
875,464 - -0.9
875,494 + +1.1
875,582 + Pf1N1B4_770 0.68 -1.5
875,582 + Pf1N1B4_770 0.68 -0.4
875,583 - Pf1N1B4_770 0.69 -1.9
875,584 + Pf1N1B4_770 0.70 -2.6
875,584 + Pf1N1B4_770 0.70 -1.5
875,584 + Pf1N1B4_770 0.70 +0.1
875,584 + Pf1N1B4_770 0.70 -0.9
875,762 + Pf1N1B4_771 0.17 +1.1
875,795 + Pf1N1B4_771 0.20 -1.2
875,871 - Pf1N1B4_771 0.29 +2.1
875,871 - Pf1N1B4_771 0.29 +0.1
876,035 + Pf1N1B4_771 0.47 -0.9
876,036 - Pf1N1B4_771 0.47 +2.0
876,036 - +1.1
876,036 - Pf1N1B4_771 0.47 -0.0
876,302 + Pf1N1B4_771 0.76 -1.5
876,303 - Pf1N1B4_771 0.76 +0.5
876,371 + Pf1N1B4_771 0.83 -0.9
876,371 + Pf1N1B4_771 0.83 -2.7
876,371 + Pf1N1B4_771 0.83 -2.5
876,371 + Pf1N1B4_771 0.83 -0.9
876,371 + Pf1N1B4_771 0.83 +0.5
876,371 + Pf1N1B4_771 0.83 +1.1
876,371 + Pf1N1B4_771 0.83 -2.7
876,371 + Pf1N1B4_771 0.83 -0.5
876,371 + Pf1N1B4_771 0.83 +0.1
876,372 - Pf1N1B4_771 0.83 -0.6
876,372 - Pf1N1B4_771 0.83 -1.5
876,372 - Pf1N1B4_771 0.83 +0.1

Or see this region's nucleotide sequence