Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_76

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotidesPf1N1B4_75 and Pf1N1B4_76 are separated by 501 nucleotidesPf1N1B4_76 and Pf1N1B4_77 are separated by 307 nucleotidesPf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429 _74 Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823 _75 Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692 _76 Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317 _77 Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994 _78 Position (kb) 79 80 81Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 78.419 kb on + strandat 78.419 kb on + strandat 78.419 kb on + strandat 78.419 kb on + strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.685 kb on + strand, within Pf1N1B4_75at 78.685 kb on + strand, within Pf1N1B4_75at 78.686 kb on - strand, within Pf1N1B4_75at 78.813 kb on - strandat 78.844 kb on + strandat 78.888 kb on + strandat 79.002 kb on + strandat 79.003 kb on - strandat 79.181 kb on + strandat 79.181 kb on + strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.200 kb on + strandat 79.201 kb on - strandat 79.236 kb on - strandat 79.276 kb on + strandat 79.276 kb on + strandat 79.277 kb on - strandat 79.277 kb on - strandat 79.324 kb on - strandat 79.587 kb on + strandat 79.785 kb on - strand, within Pf1N1B4_76at 80.281 kb on - strand, within Pf1N1B4_76at 80.281 kb on - strand, within Pf1N1B4_76at 80.344 kb on - strand, within Pf1N1B4_76at 80.374 kb on - strand, within Pf1N1B4_76at 80.449 kb on - strand, within Pf1N1B4_76at 80.503 kb on - strand, within Pf1N1B4_76at 80.659 kb on - strandat 80.705 kb on + strandat 80.752 kb on + strandat 80.752 kb on + strandat 80.752 kb on + strandat 80.752 kb on + strandat 80.753 kb on - strandat 80.753 kb on - strandat 80.753 kb on - strandat 80.753 kb on - strandat 80.824 kb on + strandat 80.824 kb on + strandat 80.824 kb on + strandat 80.825 kb on - strandat 80.825 kb on - strandat 80.877 kb on - strandat 80.877 kb on - strandat 80.911 kb on + strandat 80.927 kb on - strandat 81.030 kb on + strandat 81.030 kb on + strandat 81.031 kb on - strandat 81.071 kb on + strand, within Pf1N1B4_77at 81.071 kb on + strand, within Pf1N1B4_77at 81.071 kb on + strand, within Pf1N1B4_77at 81.137 kb on + strand, within Pf1N1B4_77at 81.162 kb on + strand, within Pf1N1B4_77at 81.220 kb on + strand, within Pf1N1B4_77at 81.289 kb on + strandat 81.290 kb on - strandat 81.290 kb on - strandat 81.315 kb on + strandat 81.316 kb on - strandat 81.334 kb on + strandat 81.335 kb on - strandat 81.335 kb on - strandat 81.403 kb on - strandat 81.403 kb on - strandat 81.426 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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78,419 + -0.2
78,419 + -0.2
78,419 + +2.6
78,419 + -1.2
78,420 - -1.2
78,420 - +0.1
78,420 - -1.6
78,420 - -0.2
78,420 - -1.5
78,685 + Pf1N1B4_75 0.63 -0.2
78,685 + Pf1N1B4_75 0.63 +0.1
78,686 - Pf1N1B4_75 0.63 -0.7
78,813 - -0.6
78,844 + +2.0
78,888 + -0.2
79,002 + +1.1
79,003 - -1.2
79,181 + -1.2
79,181 + -0.2
79,182 - -1.2
79,182 - -0.1
79,182 - -0.8
79,182 - -2.5
79,200 + +2.4
79,201 - -0.8
79,236 - -1.2
79,276 + +0.5
79,276 + -0.2
79,277 - +0.8
79,277 - +0.1
79,324 - -0.8
79,587 + -1.2
79,785 - Pf1N1B4_76 0.34 -0.2
80,281 - Pf1N1B4_76 0.70 +1.6
80,281 - Pf1N1B4_76 0.70 +0.2
80,344 - Pf1N1B4_76 0.74 +1.8
80,374 - Pf1N1B4_76 0.77 +0.8
80,449 - Pf1N1B4_76 0.82 -0.8
80,503 - Pf1N1B4_76 0.86 +1.5
80,659 - +0.2
80,705 + +0.2
80,752 + -1.8
80,752 + +1.8
80,752 + -0.2
80,752 + -3.2
80,753 - +0.8
80,753 - +0.4
80,753 - +0.8
80,753 - +2.1
80,824 + -1.2
80,824 + -0.2
80,824 + +1.2
80,825 - +1.6
80,825 - +0.8
80,877 - -1.2
80,877 - -1.2
80,911 + -0.2
80,927 - +0.5
81,030 + +1.0
81,030 + +0.8
81,031 - -1.2
81,071 + Pf1N1B4_77 0.22 -0.0
81,071 + Pf1N1B4_77 0.22 +0.8
81,071 + Pf1N1B4_77 0.22 +0.2
81,137 + Pf1N1B4_77 0.43 -2.2
81,162 + Pf1N1B4_77 0.51 +2.0
81,220 + Pf1N1B4_77 0.69 +0.5
81,289 + -0.5
81,290 - -1.8
81,290 - +1.8
81,315 + -0.8
81,316 - +0.8
81,334 + -0.0
81,335 - +1.0
81,335 - -0.8
81,403 - -1.8
81,403 - -0.5
81,426 + -0.1

Or see this region's nucleotide sequence