Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_73 and Pf1N1B4_74 are separated by 231 nucleotides Pf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotides Pf1N1B4_75 and Pf1N1B4_76 are separated by 501 nucleotides
Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934
_73
Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429
_74
Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823
_75
Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692
_76
Position (kb)
78
79 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 77.452 kb on + strand, within Pf1N1B4_73 at 77.620 kb on + strand at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.623 kb on + strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.624 kb on - strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.665 kb on + strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.666 kb on - strand, within Pf1N1B4_73 at 77.672 kb on - strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.688 kb on + strand, within Pf1N1B4_73 at 77.689 kb on - strand, within Pf1N1B4_73 at 77.689 kb on - strand, within Pf1N1B4_73 at 77.819 kb on - strand at 77.876 kb on - strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.912 kb on + strand at 77.913 kb on - strand at 77.913 kb on - strand at 78.032 kb on + strand at 78.033 kb on - strand at 78.033 kb on - strand at 78.033 kb on - strand at 78.040 kb on - strand at 78.060 kb on - strand at 78.121 kb on + strand at 78.122 kb on - strand at 78.139 kb on - strand at 78.139 kb on - strand at 78.167 kb on + strand at 78.168 kb on - strand at 78.168 kb on - strand at 78.205 kb on + strand, within Pf1N1B4_74 at 78.206 kb on - strand, within Pf1N1B4_74 at 78.206 kb on - strand, within Pf1N1B4_74 at 78.304 kb on + strand, within Pf1N1B4_74 at 78.305 kb on - strand, within Pf1N1B4_74 at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.685 kb on + strand, within Pf1N1B4_75 at 78.685 kb on + strand, within Pf1N1B4_75 at 78.686 kb on - strand, within Pf1N1B4_75 at 78.813 kb on - strand at 78.844 kb on + strand at 78.888 kb on + strand at 79.002 kb on + strand at 79.003 kb on - strand at 79.181 kb on + strand at 79.181 kb on + strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.182 kb on - strand at 79.200 kb on + strand at 79.201 kb on - strand at 79.236 kb on - strand at 79.276 kb on + strand at 79.276 kb on + strand at 79.277 kb on - strand at 79.277 kb on - strand at 79.324 kb on - strand at 79.587 kb on + strand at 79.785 kb on - strand, within Pf1N1B4_76
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 77,452 + Pf1N1B4_73 0.59 +1.6 77,620 + +0.4 77,623 + Pf1N1B4_73 0.73 +0.8 77,623 + Pf1N1B4_73 0.73 -0.8 77,623 + Pf1N1B4_73 0.73 -0.2 77,623 + Pf1N1B4_73 0.73 -0.8 77,623 + Pf1N1B4_73 0.73 -0.2 77,623 + Pf1N1B4_73 0.73 -1.2 77,624 - Pf1N1B4_73 0.73 -2.2 77,624 - Pf1N1B4_73 0.73 -0.6 77,624 - Pf1N1B4_73 0.73 -1.2 77,624 - Pf1N1B4_73 0.73 +0.4 77,665 + Pf1N1B4_73 0.77 -1.8 77,665 + Pf1N1B4_73 0.77 -1.8 77,665 + Pf1N1B4_73 0.77 +1.5 77,665 + Pf1N1B4_73 0.77 +0.1 77,665 + Pf1N1B4_73 0.77 -2.2 77,665 + Pf1N1B4_73 0.77 -1.8 77,666 - Pf1N1B4_73 0.77 +1.6 77,666 - Pf1N1B4_73 0.77 -0.8 77,666 - Pf1N1B4_73 0.77 -0.8 77,666 - Pf1N1B4_73 0.77 -0.2 77,672 - Pf1N1B4_73 0.78 -1.2 77,688 + Pf1N1B4_73 0.79 +0.5 77,688 + Pf1N1B4_73 0.79 -0.9 77,688 + Pf1N1B4_73 0.79 -2.2 77,689 - Pf1N1B4_73 0.79 +1.8 77,689 - Pf1N1B4_73 0.79 +0.1 77,819 - +0.0 77,876 - -2.2 77,912 + -0.2 77,912 + -0.2 77,912 + +0.8 77,912 + -2.3 77,913 - +0.9 77,913 - +1.4 78,032 + +0.3 78,033 - -1.2 78,033 - +0.4 78,033 - +0.6 78,040 - +0.2 78,060 - -0.2 78,121 + -2.2 78,122 - +2.8 78,139 - +1.8 78,139 - -2.2 78,167 + +1.6 78,168 - +0.4 78,168 - +1.5 78,205 + Pf1N1B4_74 0.15 +0.2 78,206 - Pf1N1B4_74 0.15 -1.8 78,206 - Pf1N1B4_74 0.15 -1.8 78,304 + Pf1N1B4_74 0.52 -0.9 78,305 - Pf1N1B4_74 0.53 -1.4 78,419 + -0.2 78,419 + -0.2 78,419 + +2.6 78,419 + -1.2 78,420 - -1.2 78,420 - +0.1 78,420 - -1.6 78,420 - -0.2 78,420 - -1.5 78,685 + Pf1N1B4_75 0.63 -0.2 78,685 + Pf1N1B4_75 0.63 +0.1 78,686 - Pf1N1B4_75 0.63 -0.7 78,813 - -0.6 78,844 + +2.0 78,888 + -0.2 79,002 + +1.1 79,003 - -1.2 79,181 + -1.2 79,181 + -0.2 79,182 - -1.2 79,182 - -0.1 79,182 - -0.8 79,182 - -2.5 79,200 + +2.4 79,201 - -0.8 79,236 - -1.2 79,276 + +0.5 79,276 + -0.2 79,277 - +0.8 79,277 - +0.1 79,324 - -0.8 79,587 + -1.2 79,785 - Pf1N1B4_76 0.34 -0.2
Or see this region's nucleotide sequence