Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_68

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotidesPf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotidesPf1N1B4_68 and Pf1N1B4_69 are separated by 293 nucleotides Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535 _68 Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542 _69 Position (kb) 70 71 72Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67at 69.798 kb on - strand, within Pf1N1B4_67at 70.129 kb on + strandat 70.160 kb on + strandat 70.161 kb on - strandat 70.534 kb on + strand, within Pf1N1B4_68at 70.596 kb on - strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68at 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.566 kb on + strandat 71.567 kb on - strandat 71.569 kb on + strandat 71.569 kb on + strandat 71.570 kb on - strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.722 kb on + strandat 71.862 kb on - strandat 71.871 kb on + strandat 71.872 kb on - strandat 72.028 kb on - strand, within Pf1N1B4_69at 72.028 kb on - strand, within Pf1N1B4_69at 72.139 kb on + strand, within Pf1N1B4_69at 72.140 kb on - strand, within Pf1N1B4_69at 72.303 kb on + strand, within Pf1N1B4_69at 72.304 kb on - strand, within Pf1N1B4_69

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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69,207 - Pf1N1B4_66 0.73 -1.2
69,207 - Pf1N1B4_66 0.73 +0.5
69,271 + Pf1N1B4_66 0.85 +3.2
69,330 + -1.2
69,330 + +0.4
69,395 + +1.8
69,432 + -0.4
69,432 + +0.3
69,433 - +2.0
69,433 - -1.2
69,433 - -0.8
69,433 - -1.8
69,473 + +1.1
69,474 - -0.6
69,474 - -2.8
69,476 - -0.6
69,476 - -2.2
69,639 - Pf1N1B4_67 0.20 -0.4
69,729 + Pf1N1B4_67 0.33 +2.2
69,798 - Pf1N1B4_67 0.43 -0.1
70,129 + +0.3
70,160 + -0.2
70,161 - -1.2
70,534 + Pf1N1B4_68 0.26 +1.3
70,596 - Pf1N1B4_68 0.30 +0.5
71,044 + Pf1N1B4_68 0.64 -0.2
71,044 + Pf1N1B4_68 0.64 -0.6
71,045 - Pf1N1B4_68 0.64 -0.2
71,045 - Pf1N1B4_68 0.64 -2.2
71,566 + +2.1
71,566 + -1.1
71,566 + -3.2
71,566 + -0.2
71,566 + -1.2
71,566 + +1.4
71,566 + -1.8
71,567 - +1.2
71,569 + -0.6
71,569 + -1.2
71,570 - +0.8
71,722 + -3.0
71,722 + -1.8
71,722 + -0.2
71,722 + -1.1
71,862 - +1.1
71,871 + +0.3
71,872 - -1.8
72,028 - Pf1N1B4_69 0.28 +2.8
72,028 - Pf1N1B4_69 0.28 +1.2
72,139 + Pf1N1B4_69 0.43 +1.1
72,140 - Pf1N1B4_69 0.44 -1.8
72,303 + Pf1N1B4_69 0.66 -2.2
72,304 - Pf1N1B4_69 0.67 -1.2

Or see this region's nucleotide sequence