Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Position (kb) 714
715
716 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand at 715.772 kb on + strand at 715.867 kb on + strand, within Pf1N1B4_619
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 713,744 - +0.0 713,933 + Pf1N1B4_617 0.20 -1.0 713,996 + -1.0 714,126 - Pf1N1B4_617 0.41 -2.0 714,126 - -1.0 714,315 - Pf1N1B4_617 0.61 -0.2 714,315 - Pf1N1B4_617 0.61 -0.3 714,660 + +0.4 714,661 - -2.3 714,661 - -2.8 714,661 - -2.0 714,661 - +0.6 714,665 + +1.4 714,665 + -0.6 714,665 + -0.2 714,665 + -1.6 714,665 + +0.9 714,665 + +2.3 714,665 + -1.6 714,665 + -2.3 714,665 + -1.6 714,665 + +0.2 714,665 + -1.6 714,665 + -1.6 714,665 + +1.8 714,665 + +0.3 714,665 + +2.3 714,665 + -2.8 714,665 + -1.0 714,666 - +0.9 714,666 - +2.9 714,666 - +1.6 714,666 - +0.0 714,666 - -1.0 714,666 - +0.3 714,666 - -0.8 714,666 - -1.0 714,666 - +1.6 714,671 + +1.6 714,671 + -2.2 714,671 + +1.6 714,672 - +1.6 714,672 - -0.6 714,672 - -1.0 714,672 - +1.0 714,752 + -1.0 714,752 + Pf1N1B4_618 0.18 -1.0 714,752 + +1.8 714,770 + Pf1N1B4_618 0.23 +0.8 714,770 + Pf1N1B4_618 0.23 -1.4 714,770 + Pf1N1B4_618 0.23 +0.0 714,770 + +0.0 714,770 + -1.0 714,770 + Pf1N1B4_618 0.23 -2.3 714,771 - Pf1N1B4_618 0.23 -1.1 714,771 - +1.3 714,771 - Pf1N1B4_618 0.23 -2.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -1.0 714,771 - Pf1N1B4_618 0.23 -2.0 714,807 + Pf1N1B4_618 0.33 -1.0 714,881 + Pf1N1B4_618 0.54 +0.0 714,881 + Pf1N1B4_618 0.54 -2.6 714,881 + Pf1N1B4_618 0.54 -1.0 714,881 + Pf1N1B4_618 0.54 -2.0 714,881 + Pf1N1B4_618 0.54 -0.6 714,920 + Pf1N1B4_618 0.64 -1.6 714,975 + Pf1N1B4_618 0.80 +1.0 715,073 + +0.1 715,227 + -2.6 715,227 + +0.0 715,228 - -1.0 715,228 - +0.3 715,379 + +1.6 715,380 - -1.6 715,772 + -1.0 715,867 + Pf1N1B4_619 0.40 +0.0
Or see this region's nucleotide sequence