Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6026 and Pf1N1B4_6027 are separated by 165 nucleotides Pf1N1B4_6027 and Pf1N1B4_6028 are separated by 131 nucleotides Pf1N1B4_6028 and Pf1N1B4_6029 are separated by 38 nucleotides Pf1N1B4_6029 and Pf1N1B4_6030 are separated by 33 nucleotides Pf1N1B4_6030 and Pf1N1B4_6031 overlap by 4 nucleotides
Pf1N1B4_6026 - lipoprotein, putative, at 492,334 to 492,651
_6026
Pf1N1B4_6027 - FIG00955358: hypothetical protein, at 492,817 to 493,122
_6027
Pf1N1B4_6028 - FIG00954052: hypothetical protein, at 493,254 to 493,460
_6028
Pf1N1B4_6029 - uridine nucleosidase (EC 3.2.2.3) (from data) , at 493,499 to 494,527
_6029
Pf1N1B4_6030 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 494,561 to 494,965
_6030
Pf1N1B4_6031 - Ribokinase (EC 2.7.1.15) (from data) , at 494,962 to 495,879
_6031
Position (kb)
493
494
495 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.671 kb on - strand at 492.674 kb on + strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.821 kb on + strand at 492.822 kb on - strand at 492.822 kb on - strand at 492.822 kb on - strand at 492.834 kb on - strand at 492.834 kb on - strand at 492.924 kb on - strand, within Pf1N1B4_6027 at 492.924 kb on - strand, within Pf1N1B4_6027 at 492.990 kb on + strand, within Pf1N1B4_6027 at 492.991 kb on - strand, within Pf1N1B4_6027 at 493.005 kb on - strand, within Pf1N1B4_6027 at 493.005 kb on - strand, within Pf1N1B4_6027 at 493.164 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.264 kb on + strand at 493.314 kb on + strand, within Pf1N1B4_6028 at 493.314 kb on + strand, within Pf1N1B4_6028 at 493.353 kb on - strand at 493.422 kb on + strand, within Pf1N1B4_6028 at 493.423 kb on - strand at 493.423 kb on - strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.436 kb on + strand, within Pf1N1B4_6028 at 493.437 kb on - strand, within Pf1N1B4_6028 at 493.500 kb on + strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.600 kb on - strand at 493.600 kb on - strand at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.653 kb on - strand at 493.696 kb on - strand, within Pf1N1B4_6029 at 493.709 kb on - strand, within Pf1N1B4_6029 at 493.764 kb on + strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.875 kb on + strand, within Pf1N1B4_6029 at 493.876 kb on - strand, within Pf1N1B4_6029 at 494.118 kb on + strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.351 kb on - strand, within Pf1N1B4_6029 at 494.517 kb on + strand at 494.518 kb on - strand at 494.518 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.571 kb on - strand at 494.571 kb on - strand at 494.599 kb on - strand at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.806 kb on + strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.974 kb on - strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.984 kb on - strand at 494.984 kb on - strand at 494.984 kb on - strand at 495.493 kb on + strand, within Pf1N1B4_6031 at 495.494 kb on - strand, within Pf1N1B4_6031
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 492,586 - Pf1N1B4_6026 0.79 +0.2 492,586 - Pf1N1B4_6026 0.79 -0.4 492,586 - Pf1N1B4_6026 0.79 +0.2 492,671 - -2.0 492,674 + -2.7 492,675 - -2.0 492,675 - -1.0 492,675 - -2.2 492,675 - -0.8 492,675 - -3.6 492,675 - -1.7 492,675 - -0.4 492,675 - -2.0 492,675 - -0.9 492,675 - -1.1 492,675 - -0.4 492,806 - -3.6 492,806 - -2.0 492,806 - +1.0 492,806 - -0.7 492,806 - -0.4 492,821 + -0.4 492,822 - -0.4 492,822 - -0.7 492,822 - -1.2 492,834 - -2.7 492,834 - -0.4 492,924 - Pf1N1B4_6027 0.35 -0.6 492,924 - Pf1N1B4_6027 0.35 -1.6 492,990 + Pf1N1B4_6027 0.57 +1.4 492,991 - Pf1N1B4_6027 0.57 +0.9 493,005 - Pf1N1B4_6027 0.61 -0.7 493,005 - Pf1N1B4_6027 0.61 -1.4 493,164 - +2.2 493,182 - -1.0 493,182 - -0.4 493,182 - +0.6 493,182 - -0.4 493,264 + +1.2 493,314 + Pf1N1B4_6028 0.29 +0.3 493,314 + Pf1N1B4_6028 0.29 +0.6 493,353 - -0.4 493,422 + Pf1N1B4_6028 0.81 -0.4 493,423 - -2.0 493,423 - Pf1N1B4_6028 0.82 -0.4 493,434 + Pf1N1B4_6028 0.87 -0.4 493,434 + Pf1N1B4_6028 0.87 +0.6 493,434 + Pf1N1B4_6028 0.87 -0.4 493,434 + Pf1N1B4_6028 0.87 -1.0 493,435 - Pf1N1B4_6028 0.87 +1.2 493,435 - -1.4 493,435 - Pf1N1B4_6028 0.87 -1.4 493,435 - Pf1N1B4_6028 0.87 -0.4 493,435 - Pf1N1B4_6028 0.87 -0.8 493,436 + Pf1N1B4_6028 0.88 -1.3 493,437 - Pf1N1B4_6028 0.88 -1.5 493,500 + -1.1 493,501 - -1.4 493,501 - -2.0 493,501 - -0.4 493,501 - +0.6 493,600 - -1.4 493,600 - -1.4 493,652 + Pf1N1B4_6029 0.15 -2.1 493,652 + Pf1N1B4_6029 0.15 +0.9 493,653 - -2.4 493,696 - Pf1N1B4_6029 0.19 +1.0 493,709 - Pf1N1B4_6029 0.20 +0.9 493,764 + Pf1N1B4_6029 0.26 -0.4 493,765 - Pf1N1B4_6029 0.26 -2.0 493,765 - Pf1N1B4_6029 0.26 -0.6 493,765 - Pf1N1B4_6029 0.26 -1.2 493,875 + Pf1N1B4_6029 0.37 -0.4 493,876 - Pf1N1B4_6029 0.37 +2.2 494,118 + Pf1N1B4_6029 0.60 +0.3 494,119 - Pf1N1B4_6029 0.60 +0.6 494,119 - Pf1N1B4_6029 0.60 -3.2 494,209 - Pf1N1B4_6029 0.69 -0.2 494,209 - -2.0 494,209 - Pf1N1B4_6029 0.69 -1.4 494,351 - Pf1N1B4_6029 0.83 -1.4 494,517 + +3.7 494,518 - -3.0 494,518 - -0.4 494,563 - -0.0 494,563 - +0.0 494,563 - +1.1 494,571 - +1.6 494,571 - -1.8 494,599 - -1.4 494,792 + Pf1N1B4_6030 0.57 -0.4 494,792 + Pf1N1B4_6030 0.57 -2.4 494,792 + Pf1N1B4_6030 0.57 -1.4 494,792 + Pf1N1B4_6030 0.57 -2.0 494,793 - Pf1N1B4_6030 0.57 -2.4 494,793 - Pf1N1B4_6030 0.57 -1.4 494,806 + Pf1N1B4_6030 0.60 +0.0 494,807 - Pf1N1B4_6030 0.61 -1.4 494,807 - Pf1N1B4_6030 0.61 -1.0 494,974 - -1.3 494,983 + -0.4 494,983 + -1.4 494,983 + +1.2 494,984 - +1.6 494,984 - +0.3 494,984 - +1.4 495,493 + Pf1N1B4_6031 0.58 -1.1 495,494 - Pf1N1B4_6031 0.58 -0.3
Or see this region's nucleotide sequence