Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5937 and Pf1N1B4_5938 overlap by 4 nucleotides Pf1N1B4_5938 and Pf1N1B4_5939 are separated by 45 nucleotides Pf1N1B4_5939 and Pf1N1B4_5940 overlap by 4 nucleotides
Pf1N1B4_5937 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 382,060 to 382,599
_5937
Pf1N1B4_5938 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 382,596 to 383,474
_5938
Pf1N1B4_5939 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 383,520 to 384,587
_5939
Pf1N1B4_5940 - Alpha-D-GlcNAc alpha-1,2-L-rhamnosyltransferase (EC 2.4.1.-), at 384,584 to 385,711
_5940
Position (kb) 383
384
385 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 382.568 kb on - strand at 382.614 kb on - strand at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 383.141 kb on - strand, within Pf1N1B4_5938 at 383.174 kb on + strand, within Pf1N1B4_5938 at 383.175 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand at 383.329 kb on - strand, within Pf1N1B4_5938 at 383.356 kb on - strand at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.390 kb on - strand at 383.413 kb on - strand at 383.427 kb on - strand at 383.597 kb on - strand at 383.738 kb on - strand at 383.738 kb on - strand, within Pf1N1B4_5939 at 384.191 kb on + strand, within Pf1N1B4_5939 at 384.192 kb on - strand, within Pf1N1B4_5939 at 384.268 kb on + strand, within Pf1N1B4_5939 at 384.268 kb on + strand, within Pf1N1B4_5939 at 384.325 kb on - strand, within Pf1N1B4_5939 at 384.325 kb on - strand, within Pf1N1B4_5939 at 384.325 kb on - strand, within Pf1N1B4_5939 at 384.325 kb on - strand, within Pf1N1B4_5939 at 384.576 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 382,568 - -1.1 382,614 - +0.2 382,865 - Pf1N1B4_5938 0.31 -0.4 382,865 - Pf1N1B4_5938 0.31 +0.6 382,949 - Pf1N1B4_5938 0.40 +1.3 382,949 - Pf1N1B4_5938 0.40 +0.9 382,955 - Pf1N1B4_5938 0.41 +0.3 382,955 - Pf1N1B4_5938 0.41 -2.0 382,955 - Pf1N1B4_5938 0.41 +2.3 383,141 - Pf1N1B4_5938 0.62 +1.8 383,174 + Pf1N1B4_5938 0.66 +2.2 383,175 - Pf1N1B4_5938 0.66 +1.7 383,322 - Pf1N1B4_5938 0.83 +1.9 383,322 - +0.6 383,329 - Pf1N1B4_5938 0.83 -3.2 383,356 - -1.0 383,372 - Pf1N1B4_5938 0.88 +0.3 383,372 - Pf1N1B4_5938 0.88 +0.6 383,372 - Pf1N1B4_5938 0.88 +1.6 383,390 - -0.4 383,413 - +2.4 383,427 - +2.4 383,597 - -2.7 383,738 - +0.2 383,738 - Pf1N1B4_5939 0.20 +1.8 384,191 + Pf1N1B4_5939 0.63 +1.0 384,192 - Pf1N1B4_5939 0.63 -0.1 384,268 + Pf1N1B4_5939 0.70 -1.4 384,268 + Pf1N1B4_5939 0.70 -2.4 384,325 - Pf1N1B4_5939 0.75 +1.2 384,325 - Pf1N1B4_5939 0.75 -1.7 384,325 - Pf1N1B4_5939 0.75 +0.9 384,325 - Pf1N1B4_5939 0.75 +2.3 384,576 - -1.4
Or see this region's nucleotide sequence