Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5884 and Pf1N1B4_5885 overlap by 4 nucleotides Pf1N1B4_5885 and Pf1N1B4_5886 overlap by 4 nucleotides Pf1N1B4_5886 and Pf1N1B4_5887 are separated by 0 nucleotides
Pf1N1B4_5884 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 327,315 to 328,667
_5884
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Pf1N1B4_5886 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 329,719 to 330,465
_5886
Pf1N1B4_5887 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 330,466 to 331,110
_5887
Position (kb) 328
329
330 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 327.879 kb on + strand, within Pf1N1B4_5884 at 327.880 kb on - strand at 328.121 kb on + strand, within Pf1N1B4_5884 at 328.266 kb on - strand, within Pf1N1B4_5884 at 328.381 kb on - strand, within Pf1N1B4_5884 at 328.417 kb on - strand, within Pf1N1B4_5884 at 328.597 kb on + strand at 328.597 kb on + strand at 328.598 kb on - strand at 328.671 kb on + strand at 328.671 kb on + strand at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand at 330.031 kb on + strand at 330.031 kb on + strand, within Pf1N1B4_5886 at 330.032 kb on - strand, within Pf1N1B4_5886 at 330.266 kb on - strand, within Pf1N1B4_5886 at 330.298 kb on + strand, within Pf1N1B4_5886 at 330.379 kb on + strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 327,879 + Pf1N1B4_5884 0.42 -1.4 327,880 - +0.9 328,121 + Pf1N1B4_5884 0.60 -0.4 328,266 - Pf1N1B4_5884 0.70 +0.2 328,381 - Pf1N1B4_5884 0.79 +0.8 328,417 - Pf1N1B4_5884 0.81 +0.4 328,597 + -1.4 328,597 + +0.0 328,598 - +1.9 328,671 + +0.2 328,671 + -2.7 328,744 - +0.7 328,773 - Pf1N1B4_5885 0.10 -0.4 328,773 - Pf1N1B4_5885 0.10 -1.4 328,938 - Pf1N1B4_5885 0.26 +1.9 328,962 - Pf1N1B4_5885 0.28 -2.4 329,036 + Pf1N1B4_5885 0.35 +0.4 329,036 + -0.4 329,036 + Pf1N1B4_5885 0.35 -2.0 329,121 - Pf1N1B4_5885 0.43 -1.0 329,162 + +0.2 329,162 + Pf1N1B4_5885 0.47 +0.2 329,162 + Pf1N1B4_5885 0.47 -1.1 329,163 - Pf1N1B4_5885 0.47 -2.0 329,163 - Pf1N1B4_5885 0.47 -0.4 329,324 + Pf1N1B4_5885 0.62 +0.7 329,324 + Pf1N1B4_5885 0.62 -2.4 329,325 - Pf1N1B4_5885 0.62 -4.1 329,325 - Pf1N1B4_5885 0.62 -0.4 329,442 - Pf1N1B4_5885 0.73 -2.4 329,442 - Pf1N1B4_5885 0.73 -1.0 329,481 - Pf1N1B4_5885 0.77 +0.3 329,660 + -1.4 329,661 - -2.4 329,663 + -1.4 330,031 + -1.4 330,031 + Pf1N1B4_5886 0.42 +0.3 330,032 - Pf1N1B4_5886 0.42 +0.4 330,266 - Pf1N1B4_5886 0.73 -0.4 330,298 + Pf1N1B4_5886 0.78 +0.0 330,379 + Pf1N1B4_5886 0.88 +1.6 330,380 - Pf1N1B4_5886 0.88 +2.2 330,380 - Pf1N1B4_5886 0.88 +0.8 330,380 - Pf1N1B4_5886 0.88 +0.1
Or see this region's nucleotide sequence