Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5786 and Pf1N1B4_5787 are separated by 219 nucleotides Pf1N1B4_5787 and Pf1N1B4_5788 are separated by 413 nucleotides Pf1N1B4_5788 and Pf1N1B4_5789 are separated by 188 nucleotides
Pf1N1B4_5786 - putative membrane protein, at 225,003 to 225,446
_5786
Pf1N1B4_5787 - Gifsy-2 prophage protein, at 225,666 to 226,358
_5787
Pf1N1B4_5788 - CBS domain protein, at 226,772 to 226,915
_5788
Pf1N1B4_5789 - hypothetical protein, at 227,104 to 227,238
_5789
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 224.781 kb on + strand at 224.781 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.954 kb on - strand at 225.016 kb on + strand at 225.016 kb on + strand at 225.017 kb on - strand at 225.226 kb on + strand at 225.330 kb on + strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.628 kb on + strand at 225.636 kb on - strand at 225.636 kb on - strand at 225.641 kb on + strand at 225.641 kb on + strand at 225.743 kb on + strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.937 kb on + strand, within Pf1N1B4_5787 at 225.938 kb on - strand, within Pf1N1B4_5787 at 225.956 kb on + strand, within Pf1N1B4_5787 at 225.957 kb on - strand at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.988 kb on - strand, within Pf1N1B4_5787 at 226.015 kb on - strand at 226.078 kb on - strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.238 kb on - strand, within Pf1N1B4_5787 at 226.487 kb on + strand at 226.607 kb on - strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.710 kb on + strand at 226.710 kb on + strand at 226.710 kb on + strand at 226.711 kb on - strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.753 kb on - strand at 226.753 kb on - strand at 226.937 kb on + strand at 226.954 kb on + strand at 227.219 kb on + strand at 227.220 kb on - strand, within Pf1N1B4_5789 at 227.268 kb on - strand at 227.331 kb on - strand at 227.331 kb on - strand at 227.331 kb on - strand at 227.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 224,781 + -0.5 224,781 + +1.1 224,953 + -0.9 224,953 + +1.1 224,953 + -2.5 224,954 - -0.5 225,016 + +0.7 225,016 + +0.1 225,017 - -2.1 225,226 + -0.5 225,330 + Pf1N1B4_5786 0.74 +0.3 225,331 - Pf1N1B4_5786 0.74 -0.2 225,331 - -1.5 225,331 - Pf1N1B4_5786 0.74 -0.7 225,331 - Pf1N1B4_5786 0.74 -1.9 225,628 + +2.1 225,636 - -2.0 225,636 - +2.3 225,641 + +0.1 225,641 + +2.7 225,743 + Pf1N1B4_5787 0.11 -1.4 225,772 - Pf1N1B4_5787 0.15 -1.5 225,772 - Pf1N1B4_5787 0.15 -1.5 225,937 + Pf1N1B4_5787 0.39 -0.9 225,938 - Pf1N1B4_5787 0.39 -1.0 225,956 + Pf1N1B4_5787 0.42 +0.9 225,957 - -1.5 225,957 - Pf1N1B4_5787 0.42 +1.9 225,957 - Pf1N1B4_5787 0.42 -0.5 225,957 - Pf1N1B4_5787 0.42 -2.8 225,988 - Pf1N1B4_5787 0.46 +1.5 226,015 - +0.5 226,078 - Pf1N1B4_5787 0.59 -0.1 226,237 + Pf1N1B4_5787 0.82 -2.5 226,237 + Pf1N1B4_5787 0.82 -2.8 226,238 - Pf1N1B4_5787 0.83 -2.5 226,487 + -1.5 226,607 - -1.0 226,656 + -1.0 226,656 + -2.0 226,656 + -2.0 226,656 + +1.5 226,656 + +0.1 226,656 + -1.5 226,656 + +1.1 226,656 + -0.9 226,656 + +1.1 226,656 + +0.7 226,656 + -3.0 226,657 - +0.2 226,657 - +0.8 226,657 - -3.5 226,657 - +0.3 226,660 + -2.0 226,660 + +1.1 226,660 + -1.5 226,660 + +2.0 226,660 + -0.5 226,660 + -2.0 226,660 + -1.5 226,660 + +0.8 226,660 + -2.0 226,660 + -0.5 226,660 + -0.2 226,710 + +1.0 226,710 + -0.0 226,710 + +1.0 226,711 - -1.5 226,752 + +1.1 226,752 + -0.5 226,752 + -2.5 226,752 + +1.9 226,753 - +0.2 226,753 - -2.5 226,937 + +0.5 226,954 + +0.5 227,219 + -0.5 227,220 - Pf1N1B4_5789 0.86 +1.7 227,268 - +0.8 227,331 - +1.1 227,331 - +2.1 227,331 - +0.9 227,335 - +1.3
Or see this region's nucleotide sequence