Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5712 and Pf1N1B4_5713 are separated by 149 nucleotides Pf1N1B4_5713 and Pf1N1B4_5714 are separated by 27 nucleotides Pf1N1B4_5714 and Pf1N1B4_5715 are separated by 108 nucleotides
Pf1N1B4_5712 - Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2), at 151,717 to 154,614
_5712
Pf1N1B4_5713 - hypothetical protein, at 154,764 to 154,979
_5713
Pf1N1B4_5714 - RND efflux system, outer membrane lipoprotein, NodT family, at 155,007 to 156,611
_5714
Pf1N1B4_5715 - RND efflux membrane fusion protein, at 156,720 to 157,754
_5715
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 154.292 kb on + strand, within Pf1N1B4_5712 at 154.572 kb on + strand at 154.573 kb on - strand at 154.594 kb on - strand at 154.594 kb on - strand at 154.807 kb on - strand, within Pf1N1B4_5713 at 154.807 kb on - strand, within Pf1N1B4_5713 at 155.080 kb on + strand at 155.081 kb on - strand at 155.500 kb on - strand, within Pf1N1B4_5714 at 155.689 kb on - strand, within Pf1N1B4_5714 at 156.019 kb on + strand, within Pf1N1B4_5714 at 156.522 kb on + strand at 156.522 kb on + strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.799 kb on + strand at 156.868 kb on + strand, within Pf1N1B4_5715 at 157.061 kb on + strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.137 kb on + strand, within Pf1N1B4_5715 at 157.137 kb on + strand, within Pf1N1B4_5715 at 157.138 kb on - strand, within Pf1N1B4_5715 at 157.138 kb on - strand at 157.396 kb on - strand, within Pf1N1B4_5715
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 154,292 + Pf1N1B4_5712 0.89 -3.2 154,572 + -1.5 154,573 - -0.9 154,594 - -0.6 154,594 - -1.5 154,807 - Pf1N1B4_5713 0.20 -0.9 154,807 - Pf1N1B4_5713 0.20 -0.1 155,080 + -0.1 155,081 - -0.5 155,500 - Pf1N1B4_5714 0.31 +0.1 155,689 - Pf1N1B4_5714 0.42 +2.3 156,019 + Pf1N1B4_5714 0.63 +0.1 156,522 + -0.5 156,522 + -2.5 156,523 - -2.8 156,523 - +1.1 156,523 - -0.5 156,523 - -2.1 156,523 - -2.1 156,799 + +2.9 156,868 + Pf1N1B4_5715 0.14 -0.3 157,061 + Pf1N1B4_5715 0.33 -1.8 157,062 - Pf1N1B4_5715 0.33 +0.9 157,062 - Pf1N1B4_5715 0.33 -1.1 157,062 - Pf1N1B4_5715 0.33 +0.5 157,062 - Pf1N1B4_5715 0.33 -1.1 157,137 + Pf1N1B4_5715 0.40 +0.5 157,137 + Pf1N1B4_5715 0.40 -2.5 157,138 - Pf1N1B4_5715 0.40 -0.4 157,138 - +0.1 157,396 - Pf1N1B4_5715 0.65 +0.5
Or see this region's nucleotide sequence