Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5686 and Pf1N1B4_5687 are separated by 132 nucleotides Pf1N1B4_5687 and Pf1N1B4_5688 are separated by 57 nucleotides Pf1N1B4_5688 and Pf1N1B4_5689 are separated by 11 nucleotides Pf1N1B4_5689 and Pf1N1B4_5690 are separated by 70 nucleotides
Pf1N1B4_5686 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,838 to 126,239
_5686
Pf1N1B4_5687 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 126,372 to 126,809
_5687
Pf1N1B4_5688 - hypothetical protein, at 126,867 to 127,196
_5688
Pf1N1B4_5689 - Lactoylglutathione lyase, at 127,208 to 127,600
_5689
Pf1N1B4_5690 - Putative TEGT family carrier/transport protein, at 127,671 to 128,387
_5690
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 125.935 kb on + strand, within Pf1N1B4_5686 at 125.936 kb on - strand, within Pf1N1B4_5686 at 126.222 kb on + strand at 126.223 kb on - strand at 126.250 kb on + strand at 126.251 kb on - strand at 126.522 kb on + strand, within Pf1N1B4_5687 at 126.523 kb on - strand, within Pf1N1B4_5687 at 126.834 kb on - strand at 126.901 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.006 kb on - strand at 127.020 kb on + strand, within Pf1N1B4_5688 at 127.068 kb on + strand, within Pf1N1B4_5688 at 127.069 kb on - strand, within Pf1N1B4_5688 at 127.074 kb on + strand at 127.074 kb on + strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.096 kb on + strand, within Pf1N1B4_5688 at 127.097 kb on - strand, within Pf1N1B4_5688 at 127.109 kb on - strand, within Pf1N1B4_5688 at 127.155 kb on + strand, within Pf1N1B4_5688 at 127.255 kb on - strand at 127.263 kb on - strand, within Pf1N1B4_5689 at 127.460 kb on + strand, within Pf1N1B4_5689 at 127.544 kb on + strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand, within Pf1N1B4_5689 at 127.545 kb on - strand at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.546 kb on + strand, within Pf1N1B4_5689 at 127.630 kb on - strand at 127.727 kb on - strand at 127.727 kb on - strand at 127.735 kb on - strand at 127.735 kb on - strand at 127.739 kb on - strand at 127.763 kb on - strand, within Pf1N1B4_5690 at 127.763 kb on - strand, within Pf1N1B4_5690 at 127.966 kb on + strand, within Pf1N1B4_5690 at 127.966 kb on + strand, within Pf1N1B4_5690 at 127.967 kb on - strand, within Pf1N1B4_5690
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 125,935 + Pf1N1B4_5686 0.24 +0.1 125,936 - Pf1N1B4_5686 0.24 -2.1 126,222 + +2.2 126,223 - +1.1 126,250 + +1.9 126,251 - -0.5 126,522 + Pf1N1B4_5687 0.34 +0.9 126,523 - Pf1N1B4_5687 0.34 +0.5 126,834 - +1.1 126,901 + Pf1N1B4_5688 0.10 -1.5 127,005 + Pf1N1B4_5688 0.42 +0.3 127,005 + Pf1N1B4_5688 0.42 +2.3 127,005 + Pf1N1B4_5688 0.42 -1.1 127,006 - +0.5 127,020 + Pf1N1B4_5688 0.46 -0.8 127,068 + Pf1N1B4_5688 0.61 -0.5 127,069 - Pf1N1B4_5688 0.61 -3.3 127,074 + -1.5 127,074 + Pf1N1B4_5688 0.63 -0.5 127,075 - Pf1N1B4_5688 0.63 -1.1 127,075 - Pf1N1B4_5688 0.63 +0.1 127,096 + Pf1N1B4_5688 0.69 +0.1 127,097 - Pf1N1B4_5688 0.70 -2.1 127,109 - Pf1N1B4_5688 0.73 +0.8 127,155 + Pf1N1B4_5688 0.87 -0.5 127,255 - -0.5 127,263 - Pf1N1B4_5689 0.14 +1.3 127,460 + Pf1N1B4_5689 0.64 -0.5 127,544 + Pf1N1B4_5689 0.85 -1.8 127,545 - Pf1N1B4_5689 0.86 +0.4 127,545 - Pf1N1B4_5689 0.86 +1.3 127,545 - Pf1N1B4_5689 0.86 -1.1 127,545 - +1.5 127,546 + Pf1N1B4_5689 0.86 +1.9 127,546 + Pf1N1B4_5689 0.86 +0.7 127,546 + Pf1N1B4_5689 0.86 -0.2 127,630 - -0.9 127,727 - +0.4 127,727 - -0.5 127,735 - +0.5 127,735 - -1.2 127,739 - -0.5 127,763 - Pf1N1B4_5690 0.13 -0.3 127,763 - Pf1N1B4_5690 0.13 +1.5 127,966 + Pf1N1B4_5690 0.41 -2.5 127,966 + Pf1N1B4_5690 0.41 +0.7 127,967 - Pf1N1B4_5690 0.41 +0.9
Or see this region's nucleotide sequence