Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_560 and Pf1N1B4_561 are separated by 99 nucleotides Pf1N1B4_561 and Pf1N1B4_562 are separated by 143 nucleotides Pf1N1B4_562 and Pf1N1B4_563 overlap by 4 nucleotides Pf1N1B4_563 and Pf1N1B4_564 are separated by 233 nucleotides
Pf1N1B4_560 - Transcriptional regulators, LysR family, at 657,019 to 657,951
_560
Pf1N1B4_561 - Glutathione S-transferase (EC 2.5.1.18), at 658,051 to 658,653
_561
Pf1N1B4_562 - Histidine transporter, periplasmic histidine-binding protein, at 658,797 to 659,810
_562
Pf1N1B4_563 - RNA polymerase sigma-70 factor, at 659,807 to 660,355
_563
Pf1N1B4_564 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 660,589 to 661,353
_564
Position (kb) 658
659
660 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 657.957 kb on + strand at 657.958 kb on - strand at 658.013 kb on + strand at 658.013 kb on + strand at 658.013 kb on + strand at 658.014 kb on - strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.060 kb on + strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.061 kb on - strand at 658.264 kb on + strand, within Pf1N1B4_561 at 658.265 kb on - strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand at 658.483 kb on + strand at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.483 kb on + strand, within Pf1N1B4_561 at 658.519 kb on + strand, within Pf1N1B4_561 at 658.519 kb on + strand, within Pf1N1B4_561 at 658.520 kb on - strand, within Pf1N1B4_561 at 658.527 kb on + strand, within Pf1N1B4_561 at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.711 kb on + strand at 658.712 kb on - strand at 658.712 kb on - strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.713 kb on + strand at 658.714 kb on - strand at 658.714 kb on - strand at 658.714 kb on - strand at 658.776 kb on + strand at 658.777 kb on - strand at 658.777 kb on - strand at 658.786 kb on + strand at 658.786 kb on + strand at 658.786 kb on + strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.787 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 658.799 kb on - strand at 659.165 kb on - strand, within Pf1N1B4_562 at 659.165 kb on - strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.248 kb on + strand, within Pf1N1B4_562 at 659.249 kb on - strand, within Pf1N1B4_562 at 659.249 kb on - strand, within Pf1N1B4_562 at 659.266 kb on + strand at 659.267 kb on - strand, within Pf1N1B4_562 at 659.267 kb on - strand, within Pf1N1B4_562 at 659.418 kb on - strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.449 kb on + strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.450 kb on - strand, within Pf1N1B4_562 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.892 kb on + strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.893 kb on - strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 659.943 kb on + strand, within Pf1N1B4_563 at 660.138 kb on + strand, within Pf1N1B4_563 at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.355 kb on + strand at 660.356 kb on - strand at 660.356 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.412 kb on - strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.485 kb on + strand at 660.486 kb on - strand at 660.486 kb on - strand at 660.651 kb on - strand at 660.785 kb on + strand, within Pf1N1B4_564 at 660.785 kb on + strand, within Pf1N1B4_564 at 660.786 kb on - strand, within Pf1N1B4_564
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 657,957 + +1.2 657,958 - +1.1 658,013 + -1.0 658,013 + -2.0 658,013 + -0.0 658,014 - +0.4 658,060 + -0.0 658,060 + -1.6 658,060 + -0.6 658,060 + -1.0 658,060 + -1.6 658,060 + -2.0 658,060 + -2.0 658,061 - +0.2 658,061 - +1.8 658,061 - -2.6 658,061 - -1.6 658,061 - +1.6 658,264 + Pf1N1B4_561 0.35 -1.0 658,265 - Pf1N1B4_561 0.35 -1.0 658,483 + Pf1N1B4_561 0.72 -1.0 658,483 + +2.0 658,483 + -0.0 658,483 + Pf1N1B4_561 0.72 -2.6 658,483 + Pf1N1B4_561 0.72 +1.4 658,483 + Pf1N1B4_561 0.72 -1.0 658,519 + Pf1N1B4_561 0.78 -0.0 658,519 + Pf1N1B4_561 0.78 -1.0 658,520 - Pf1N1B4_561 0.78 -0.3 658,527 + Pf1N1B4_561 0.79 -0.6 658,711 + -0.6 658,711 + +1.2 658,711 + -1.6 658,711 + +2.1 658,711 + -0.8 658,711 + +0.7 658,711 + -0.6 658,711 + -1.6 658,711 + -1.0 658,711 + +0.9 658,711 + +2.1 658,712 - -1.6 658,712 - +2.4 658,713 + -0.0 658,713 + -0.6 658,713 + +0.8 658,713 + -1.0 658,713 + -2.6 658,714 - -0.4 658,714 - -0.0 658,714 - -0.0 658,776 + -1.0 658,777 - -1.0 658,777 - +0.6 658,786 + +1.6 658,786 + -2.3 658,786 + +0.7 658,787 - +0.6 658,787 - -2.0 658,787 - -0.0 658,787 - -1.0 658,787 - -1.0 658,799 - -0.6 658,799 - -0.6 658,799 - -0.5 658,799 - -2.0 659,165 - Pf1N1B4_562 0.36 +1.2 659,165 - Pf1N1B4_562 0.36 +0.1 659,248 + Pf1N1B4_562 0.44 +0.2 659,248 + Pf1N1B4_562 0.44 -2.0 659,248 + Pf1N1B4_562 0.44 -1.6 659,249 - Pf1N1B4_562 0.45 -0.0 659,249 - Pf1N1B4_562 0.45 -0.0 659,266 + -0.0 659,267 - Pf1N1B4_562 0.46 -0.0 659,267 - Pf1N1B4_562 0.46 +0.6 659,418 - Pf1N1B4_562 0.61 +1.3 659,449 + Pf1N1B4_562 0.64 +1.6 659,449 + Pf1N1B4_562 0.64 +1.6 659,449 + Pf1N1B4_562 0.64 +1.0 659,450 - Pf1N1B4_562 0.64 -3.2 659,450 - Pf1N1B4_562 0.64 +1.6 659,450 - Pf1N1B4_562 0.64 -1.3 659,450 - Pf1N1B4_562 0.64 -0.0 659,892 + Pf1N1B4_563 0.15 -0.0 659,892 + Pf1N1B4_563 0.15 -1.6 659,892 + Pf1N1B4_563 0.15 -1.6 659,892 + Pf1N1B4_563 0.15 +0.8 659,893 - Pf1N1B4_563 0.16 -0.3 659,893 - Pf1N1B4_563 0.16 +1.0 659,943 + Pf1N1B4_563 0.25 -0.0 659,943 + Pf1N1B4_563 0.25 -1.6 660,138 + Pf1N1B4_563 0.60 -1.0 660,355 + -0.6 660,355 + +0.6 660,355 + +0.5 660,355 + -0.5 660,355 + +1.3 660,356 - +1.6 660,356 - +2.1 660,412 - -3.0 660,412 - +0.6 660,412 - -0.8 660,412 - -0.5 660,485 + -0.0 660,485 + +1.0 660,485 + -0.0 660,485 + +0.8 660,485 + -0.8 660,485 + -0.0 660,485 + -1.2 660,485 + +1.6 660,485 + -0.6 660,486 - +0.4 660,486 - +1.0 660,651 - -1.0 660,785 + Pf1N1B4_564 0.26 -0.2 660,785 + Pf1N1B4_564 0.26 +0.2 660,786 - Pf1N1B4_564 0.26 +0.8
Or see this region's nucleotide sequence