Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5548 and Pf1N1B4_5549 are separated by 989 nucleotides Pf1N1B4_5549 and Pf1N1B4_5550 are separated by 322 nucleotides
Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943
_5548
Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061
_5549
Pf1N1B4_5550 - Group 2 RNA polymerase sigma factor @ Cyanobacteria-specific RpoD-like sigma factor, type-7, at 321,384 to 322,937
_5550
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 319.947 kb on + strand at 319.948 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.966 kb on + strand at 319.971 kb on + strand at 319.975 kb on - strand at 319.975 kb on - strand at 320.021 kb on + strand at 320.022 kb on - strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.174 kb on + strand at 320.174 kb on + strand at 320.175 kb on - strand at 320.175 kb on - strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.179 kb on - strand at 320.179 kb on - strand at 320.200 kb on - strand at 320.255 kb on + strand at 320.256 kb on - strand at 320.277 kb on - strand at 320.282 kb on - strand at 320.339 kb on + strand at 320.339 kb on + strand at 320.340 kb on - strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.367 kb on + strand at 320.367 kb on + strand at 320.368 kb on - strand at 320.409 kb on - strand at 320.558 kb on + strand at 320.558 kb on + strand at 320.559 kb on - strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.681 kb on + strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.735 kb on - strand at 320.787 kb on + strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.848 kb on + strand at 320.849 kb on - strand at 320.935 kb on - strand at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 321.053 kb on - strand at 321.053 kb on - strand at 321.208 kb on + strand at 321.223 kb on + strand at 321.427 kb on - strand at 321.427 kb on - strand at 321.435 kb on + strand at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.724 kb on + strand, within Pf1N1B4_5550 at 321.724 kb on + strand at 321.734 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.958 kb on + strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 322.013 kb on + strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.031 kb on - strand, within Pf1N1B4_5550
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 319,947 + -0.1 319,948 - -0.3 319,954 - -1.7 319,954 - -2.4 319,954 - +0.9 319,954 - -2.1 319,966 + -0.1 319,971 + -1.1 319,975 - -2.1 319,975 - +0.7 320,021 + -1.7 320,022 - +0.4 320,135 + -1.7 320,135 + -1.7 320,135 + -1.1 320,135 + -0.1 320,135 + -2.4 320,135 + +0.9 320,135 + -0.1 320,136 - +1.0 320,136 - +0.9 320,136 - -2.4 320,136 - +1.3 320,136 - -0.5 320,174 + +1.5 320,174 + -2.9 320,175 - -1.1 320,175 - -0.3 320,178 + -0.1 320,178 + -1.7 320,178 + +2.1 320,178 + -1.8 320,178 + +1.7 320,179 - -2.4 320,179 - -1.7 320,200 - +0.2 320,255 + -2.0 320,256 - +0.1 320,277 - -0.1 320,282 - -2.7 320,339 + -0.1 320,339 + -1.1 320,340 - -0.8 320,365 + -0.1 320,365 + +1.2 320,365 + -1.4 320,366 - +0.7 320,366 - -1.1 320,366 - +1.2 320,366 - -0.8 320,366 - -0.8 320,367 + -0.8 320,367 + -0.1 320,368 - +0.5 320,409 - +0.7 320,558 + -1.1 320,558 + +0.6 320,559 - -2.1 320,622 + -0.5 320,622 + +0.5 320,622 + -0.8 320,623 - -0.1 320,623 - -1.1 320,623 - -2.1 320,681 + -1.7 320,682 - +1.2 320,682 - -1.7 320,682 - +0.9 320,685 + -0.3 320,685 + +1.1 320,685 + +0.2 320,685 + -1.1 320,685 + -1.2 320,685 + +1.2 320,686 - -1.1 320,686 - +0.2 320,686 - -1.7 320,686 - +0.3 320,686 - +0.9 320,686 - -0.1 320,735 - -1.1 320,787 + -1.5 320,788 - -0.5 320,788 - -1.1 320,848 + -0.5 320,849 - -2.4 320,935 - -0.8 320,995 + Pf1N1B4_5549 0.48 +1.5 320,995 + Pf1N1B4_5549 0.48 +0.9 320,995 + Pf1N1B4_5549 0.48 +0.8 320,996 - Pf1N1B4_5549 0.49 -0.1 320,996 - Pf1N1B4_5549 0.49 +2.2 320,996 - Pf1N1B4_5549 0.49 -0.4 320,996 - Pf1N1B4_5549 0.49 -1.1 321,053 - +0.3 321,053 - -0.1 321,208 + -0.1 321,223 + +1.7 321,427 - -0.5 321,427 - -2.1 321,435 + -0.1 321,595 + Pf1N1B4_5550 0.14 -2.1 321,595 + Pf1N1B4_5550 0.14 +0.4 321,596 - Pf1N1B4_5550 0.14 -2.4 321,596 - Pf1N1B4_5550 0.14 +2.1 321,596 - Pf1N1B4_5550 0.14 -2.1 321,596 - Pf1N1B4_5550 0.14 -0.7 321,596 - Pf1N1B4_5550 0.14 -1.1 321,628 - Pf1N1B4_5550 0.16 -0.1 321,628 - Pf1N1B4_5550 0.16 -1.7 321,724 + Pf1N1B4_5550 0.22 +2.9 321,724 + -1.1 321,734 - Pf1N1B4_5550 0.23 -1.7 321,926 - Pf1N1B4_5550 0.35 -0.1 321,926 - Pf1N1B4_5550 0.35 -2.9 321,926 - Pf1N1B4_5550 0.35 -0.1 321,958 + Pf1N1B4_5550 0.37 -2.4 321,959 - Pf1N1B4_5550 0.37 +1.2 321,959 - Pf1N1B4_5550 0.37 +1.1 321,959 - Pf1N1B4_5550 0.37 -2.1 322,013 + Pf1N1B4_5550 0.40 -2.4 322,014 - Pf1N1B4_5550 0.41 +1.3 322,014 - Pf1N1B4_5550 0.41 -0.7 322,014 - Pf1N1B4_5550 0.41 +0.9 322,031 - Pf1N1B4_5550 0.42 +0.1
Or see this region's nucleotide sequence