Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5546

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5545 and Pf1N1B4_5546 overlap by 1 nucleotidesPf1N1B4_5546 and Pf1N1B4_5547 are separated by 37 nucleotidesPf1N1B4_5547 and Pf1N1B4_5548 are separated by 150 nucleotides Pf1N1B4_5545 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 311,793 to 317,519 _5545 Pf1N1B4_5546 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 317,519 to 318,460 _5546 Pf1N1B4_5547 - hypothetical protein, at 318,498 to 318,779 _5547 Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943 _5548 Position (kb) 317 318 319Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 316.586 kb on + strand, within Pf1N1B4_5545at 316.586 kb on + strand, within Pf1N1B4_5545at 316.587 kb on - strand, within Pf1N1B4_5545at 316.705 kb on - strand, within Pf1N1B4_5545at 316.786 kb on - strand, within Pf1N1B4_5545at 316.786 kb on - strand, within Pf1N1B4_5545at 316.786 kb on - strand, within Pf1N1B4_5545at 316.807 kb on + strandat 316.807 kb on + strand, within Pf1N1B4_5545at 316.850 kb on + strand, within Pf1N1B4_5545at 316.850 kb on + strand, within Pf1N1B4_5545at 316.851 kb on - strand, within Pf1N1B4_5545at 316.851 kb on - strand, within Pf1N1B4_5545at 316.978 kb on - strandat 316.985 kb on + strandat 317.192 kb on + strandat 317.192 kb on + strandat 317.197 kb on - strandat 317.256 kb on - strandat 317.284 kb on + strandat 317.284 kb on + strandat 317.285 kb on - strandat 317.461 kb on + strandat 317.486 kb on + strandat 317.486 kb on + strandat 317.486 kb on + strandat 317.487 kb on - strandat 317.518 kb on - strandat 317.532 kb on + strandat 317.533 kb on - strandat 317.533 kb on - strandat 317.533 kb on - strandat 317.823 kb on + strand, within Pf1N1B4_5546at 317.878 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.893 kb on - strand, within Pf1N1B4_5546at 317.940 kb on + strand, within Pf1N1B4_5546at 317.948 kb on + strand, within Pf1N1B4_5546at 317.948 kb on + strand, within Pf1N1B4_5546at 317.985 kb on + strand, within Pf1N1B4_5546at 317.998 kb on + strand, within Pf1N1B4_5546at 317.998 kb on + strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 318.105 kb on + strand, within Pf1N1B4_5546at 318.130 kb on + strand, within Pf1N1B4_5546at 318.131 kb on - strand, within Pf1N1B4_5546at 318.167 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.250 kb on - strand, within Pf1N1B4_5546at 318.253 kb on + strandat 318.253 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.316 kb on - strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.432 kb on + strandat 318.440 kb on + strandat 318.610 kb on - strand, within Pf1N1B4_5547at 318.651 kb on - strand, within Pf1N1B4_5547at 318.896 kb on + strandat 318.897 kb on - strandat 318.920 kb on + strandat 318.986 kb on - strandat 318.986 kb on - strandat 318.986 kb on - strandat 318.986 kb on - strandat 319.027 kb on + strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.044 kb on + strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.182 kb on + strand, within Pf1N1B4_5548at 319.360 kb on - strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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316,586 + Pf1N1B4_5545 0.84 -0.2
316,586 + Pf1N1B4_5545 0.84 -0.1
316,587 - Pf1N1B4_5545 0.84 +2.9
316,705 - Pf1N1B4_5545 0.86 +0.5
316,786 - Pf1N1B4_5545 0.87 -2.7
316,786 - Pf1N1B4_5545 0.87 -0.4
316,786 - Pf1N1B4_5545 0.87 +1.1
316,807 + +0.3
316,807 + Pf1N1B4_5545 0.88 +1.9
316,850 + Pf1N1B4_5545 0.88 -0.3
316,850 + Pf1N1B4_5545 0.88 -1.7
316,851 - Pf1N1B4_5545 0.88 -0.3
316,851 - Pf1N1B4_5545 0.88 -1.7
316,978 - +2.1
316,985 + -2.9
317,192 + -1.7
317,192 + -0.1
317,197 - -0.1
317,256 - -1.7
317,284 + -2.1
317,284 + -0.8
317,285 - -0.9
317,461 + -1.2
317,486 + -1.1
317,486 + -0.6
317,486 + -1.1
317,487 - +0.1
317,518 - -0.1
317,532 + -1.7
317,533 - +0.3
317,533 - +1.5
317,533 - -1.1
317,823 + Pf1N1B4_5546 0.32 -0.4
317,878 + Pf1N1B4_5546 0.38 -2.9
317,892 + Pf1N1B4_5546 0.40 -0.1
317,892 + Pf1N1B4_5546 0.40 -1.1
317,892 + Pf1N1B4_5546 0.40 +0.8
317,893 - Pf1N1B4_5546 0.40 +2.2
317,940 + Pf1N1B4_5546 0.45 +1.1
317,948 + Pf1N1B4_5546 0.46 -0.1
317,948 + Pf1N1B4_5546 0.46 -0.1
317,985 + Pf1N1B4_5546 0.49 -0.1
317,998 + Pf1N1B4_5546 0.51 -0.4
317,998 + Pf1N1B4_5546 0.51 +0.2
317,999 - Pf1N1B4_5546 0.51 -2.1
317,999 - Pf1N1B4_5546 0.51 +1.1
317,999 - Pf1N1B4_5546 0.51 -2.0
318,105 + Pf1N1B4_5546 0.62 -1.7
318,130 + Pf1N1B4_5546 0.65 +1.7
318,131 - Pf1N1B4_5546 0.65 -0.1
318,167 + Pf1N1B4_5546 0.69 +0.3
318,249 + Pf1N1B4_5546 0.77 +0.3
318,249 + Pf1N1B4_5546 0.77 -1.4
318,249 + Pf1N1B4_5546 0.77 -0.1
318,249 + Pf1N1B4_5546 0.77 -3.2
318,250 - Pf1N1B4_5546 0.78 -0.7
318,253 + +0.9
318,253 + Pf1N1B4_5546 0.78 +2.2
318,315 + Pf1N1B4_5546 0.85 -1.4
318,315 + Pf1N1B4_5546 0.85 -1.1
318,315 + Pf1N1B4_5546 0.85 +0.9
318,315 + Pf1N1B4_5546 0.85 -2.1
318,316 - Pf1N1B4_5546 0.85 +2.1
318,358 + Pf1N1B4_5546 0.89 -0.1
318,358 + Pf1N1B4_5546 0.89 -0.1
318,358 + Pf1N1B4_5546 0.89 -1.1
318,358 + Pf1N1B4_5546 0.89 -2.7
318,358 + Pf1N1B4_5546 0.89 -0.5
318,359 - Pf1N1B4_5546 0.89 -0.2
318,359 - Pf1N1B4_5546 0.89 +0.5
318,359 - Pf1N1B4_5546 0.89 -1.1
318,359 - Pf1N1B4_5546 0.89 +0.3
318,432 + -1.7
318,440 + -1.7
318,610 - Pf1N1B4_5547 0.40 -0.7
318,651 - Pf1N1B4_5547 0.54 +1.5
318,896 + +1.1
318,897 - +1.0
318,920 + +0.1
318,986 - +0.1
318,986 - +1.2
318,986 - +1.5
318,986 - -1.7
319,027 + -1.1
319,028 - -1.1
319,028 - +0.1
319,028 - -1.4
319,028 - +2.2
319,044 + Pf1N1B4_5548 0.11 +1.1
319,060 - Pf1N1B4_5548 0.13 +0.7
319,060 - Pf1N1B4_5548 0.13 +0.9
319,060 - Pf1N1B4_5548 0.13 -1.7
319,182 + Pf1N1B4_5548 0.25 +1.8
319,360 - Pf1N1B4_5548 0.42 +1.1
319,408 + Pf1N1B4_5548 0.47 -1.7
319,408 + Pf1N1B4_5548 0.47 -1.4
319,408 + Pf1N1B4_5548 0.47 +0.3
319,408 + Pf1N1B4_5548 0.47 +0.1

Or see this region's nucleotide sequence