Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_548 and Pf1N1B4_549 are separated by 14 nucleotides Pf1N1B4_549 and Pf1N1B4_550 are separated by 3 nucleotides Pf1N1B4_550 and Pf1N1B4_551 are separated by 93 nucleotides
Pf1N1B4_548 - Transcriptional regulator, LysR family, at 645,356 to 646,279
_548
Pf1N1B4_549 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 646,294 to 647,097
_549
Pf1N1B4_550 - Peptidyl-prolyl cis-trans isomerase PpiA precursor (EC 5.2.1.8), at 647,101 to 647,658
_550
Pf1N1B4_551 - Lipid A export ATP-binding/permease protein MsbA, at 647,752 to 649,584
_551
Position (kb) 647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 646.122 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.166 kb on + strand, within Pf1N1B4_548 at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.330 kb on + strand at 646.451 kb on + strand, within Pf1N1B4_549 at 646.558 kb on + strand at 646.558 kb on + strand, within Pf1N1B4_549 at 646.558 kb on + strand, within Pf1N1B4_549 at 646.558 kb on + strand, within Pf1N1B4_549 at 646.558 kb on + strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.559 kb on - strand, within Pf1N1B4_549 at 646.562 kb on + strand, within Pf1N1B4_549 at 646.562 kb on + strand, within Pf1N1B4_549 at 646.562 kb on + strand, within Pf1N1B4_549 at 646.562 kb on + strand, within Pf1N1B4_549 at 646.562 kb on + strand, within Pf1N1B4_549 at 646.563 kb on - strand, within Pf1N1B4_549 at 646.563 kb on - strand, within Pf1N1B4_549 at 646.632 kb on - strand at 646.632 kb on - strand, within Pf1N1B4_549 at 646.632 kb on - strand, within Pf1N1B4_549 at 646.834 kb on + strand at 646.834 kb on + strand, within Pf1N1B4_549 at 646.835 kb on - strand, within Pf1N1B4_549 at 646.835 kb on - strand, within Pf1N1B4_549 at 646.860 kb on - strand, within Pf1N1B4_549 at 646.860 kb on - strand, within Pf1N1B4_549 at 646.860 kb on - strand, within Pf1N1B4_549 at 646.860 kb on - strand at 646.957 kb on + strand, within Pf1N1B4_549 at 646.961 kb on + strand, within Pf1N1B4_549 at 646.961 kb on + strand, within Pf1N1B4_549 at 646.961 kb on + strand, within Pf1N1B4_549 at 646.962 kb on - strand, within Pf1N1B4_549 at 646.962 kb on - strand, within Pf1N1B4_549 at 647.100 kb on + strand at 647.100 kb on + strand at 647.101 kb on - strand at 647.101 kb on - strand at 647.101 kb on - strand at 647.101 kb on - strand at 647.269 kb on + strand, within Pf1N1B4_550 at 647.269 kb on + strand, within Pf1N1B4_550 at 647.269 kb on + strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.270 kb on - strand, within Pf1N1B4_550 at 647.287 kb on + strand, within Pf1N1B4_550 at 647.288 kb on - strand, within Pf1N1B4_550 at 647.288 kb on - strand, within Pf1N1B4_550 at 647.325 kb on + strand at 647.325 kb on + strand, within Pf1N1B4_550 at 647.325 kb on + strand, within Pf1N1B4_550 at 647.326 kb on - strand, within Pf1N1B4_550 at 647.413 kb on - strand, within Pf1N1B4_550 at 647.522 kb on - strand, within Pf1N1B4_550 at 647.662 kb on + strand at 647.662 kb on + strand at 647.662 kb on + strand at 647.663 kb on - strand at 647.714 kb on - strand at 647.714 kb on - strand at 647.714 kb on - strand at 647.721 kb on - strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.836 kb on + strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.837 kb on - strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.838 kb on + strand at 647.839 kb on - strand at 647.839 kb on - strand at 647.839 kb on - strand at 647.839 kb on - strand at 647.839 kb on - strand at 647.839 kb on - strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.842 kb on + strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.843 kb on - strand at 647.845 kb on + strand at 647.845 kb on + strand at 647.845 kb on + strand at 647.845 kb on + strand at 647.846 kb on - strand at 647.929 kb on + strand at 647.930 kb on - strand at 647.930 kb on - strand at 648.068 kb on + strand, within Pf1N1B4_551 at 648.069 kb on - strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.124 kb on + strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.125 kb on - strand, within Pf1N1B4_551 at 648.353 kb on - strand at 648.355 kb on + strand, within Pf1N1B4_551 at 648.355 kb on + strand, within Pf1N1B4_551 at 648.355 kb on + strand, within Pf1N1B4_551 at 648.355 kb on + strand, within Pf1N1B4_551 at 648.355 kb on + strand, within Pf1N1B4_551 at 648.356 kb on - strand, within Pf1N1B4_551 at 648.356 kb on - strand, within Pf1N1B4_551 at 648.356 kb on - strand, within Pf1N1B4_551 at 648.436 kb on + strand, within Pf1N1B4_551 at 648.436 kb on + strand, within Pf1N1B4_551 at 648.490 kb on + strand, within Pf1N1B4_551 at 648.491 kb on - strand, within Pf1N1B4_551
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 646,122 + Pf1N1B4_548 0.83 -2.3 646,160 + Pf1N1B4_548 0.87 +1.0 646,160 + Pf1N1B4_548 0.87 -2.0 646,160 + Pf1N1B4_548 0.87 -1.2 646,161 - Pf1N1B4_548 0.87 -2.0 646,161 - Pf1N1B4_548 0.87 -1.3 646,161 - Pf1N1B4_548 0.87 +0.7 646,161 - Pf1N1B4_548 0.87 -1.0 646,161 - Pf1N1B4_548 0.87 -0.3 646,161 - Pf1N1B4_548 0.87 -0.0 646,161 - Pf1N1B4_548 0.87 -0.0 646,166 + Pf1N1B4_548 0.88 -1.3 646,289 + -0.3 646,289 + -0.4 646,289 + -1.0 646,289 + +0.7 646,289 + -0.3 646,289 + +1.4 646,289 + -2.3 646,289 + +0.3 646,289 + -0.6 646,289 + -1.6 646,289 + -0.0 646,289 + -0.8 646,290 - -2.0 646,290 - -1.3 646,290 - -0.0 646,290 - -1.0 646,290 - +1.3 646,290 - -0.0 646,290 - +0.6 646,290 - -0.0 646,290 - -0.0 646,290 - +1.0 646,290 - +1.8 646,290 - +0.6 646,290 - +1.3 646,290 - -1.0 646,293 + -0.7 646,293 + -0.0 646,293 + -0.0 646,293 + -0.3 646,293 + -3.3 646,293 + +1.2 646,293 + -0.0 646,294 - +1.1 646,294 - -1.2 646,294 - -0.8 646,294 - -1.6 646,330 + -1.0 646,451 + Pf1N1B4_549 0.20 -2.3 646,558 + -1.0 646,558 + Pf1N1B4_549 0.33 +1.8 646,558 + Pf1N1B4_549 0.33 -1.6 646,558 + Pf1N1B4_549 0.33 -1.0 646,558 + Pf1N1B4_549 0.33 +1.0 646,559 - Pf1N1B4_549 0.33 -2.3 646,559 - Pf1N1B4_549 0.33 -1.0 646,559 - Pf1N1B4_549 0.33 +0.7 646,559 - Pf1N1B4_549 0.33 +1.4 646,559 - Pf1N1B4_549 0.33 -1.0 646,559 - Pf1N1B4_549 0.33 -0.6 646,559 - Pf1N1B4_549 0.33 +0.5 646,559 - Pf1N1B4_549 0.33 +0.6 646,559 - Pf1N1B4_549 0.33 -2.6 646,559 - -0.0 646,559 - Pf1N1B4_549 0.33 -2.8 646,559 - Pf1N1B4_549 0.33 +0.7 646,559 - Pf1N1B4_549 0.33 -0.0 646,559 - Pf1N1B4_549 0.33 +0.6 646,559 - Pf1N1B4_549 0.33 -2.8 646,559 - Pf1N1B4_549 0.33 +1.6 646,559 - Pf1N1B4_549 0.33 +1.4 646,562 + Pf1N1B4_549 0.33 -1.0 646,562 + Pf1N1B4_549 0.33 -2.3 646,562 + Pf1N1B4_549 0.33 +0.9 646,562 + Pf1N1B4_549 0.33 -1.3 646,562 + Pf1N1B4_549 0.33 -2.3 646,563 - Pf1N1B4_549 0.33 +0.3 646,563 - Pf1N1B4_549 0.33 -0.4 646,632 - -1.6 646,632 - Pf1N1B4_549 0.42 +1.5 646,632 - Pf1N1B4_549 0.42 -0.1 646,834 + +2.0 646,834 + Pf1N1B4_549 0.67 -0.0 646,835 - Pf1N1B4_549 0.67 -1.0 646,835 - Pf1N1B4_549 0.67 +0.3 646,860 - Pf1N1B4_549 0.70 +0.6 646,860 - Pf1N1B4_549 0.70 -0.0 646,860 - Pf1N1B4_549 0.70 -1.3 646,860 - -1.0 646,957 + Pf1N1B4_549 0.82 -1.0 646,961 + Pf1N1B4_549 0.83 -0.6 646,961 + Pf1N1B4_549 0.83 -0.0 646,961 + Pf1N1B4_549 0.83 +0.4 646,962 - Pf1N1B4_549 0.83 +2.0 646,962 - Pf1N1B4_549 0.83 -0.0 647,100 + -0.3 647,100 + +0.6 647,101 - -0.6 647,101 - -1.0 647,101 - -1.6 647,101 - -1.0 647,269 + Pf1N1B4_550 0.30 -3.0 647,269 + Pf1N1B4_550 0.30 -1.6 647,269 + Pf1N1B4_550 0.30 -0.3 647,270 - Pf1N1B4_550 0.30 -1.6 647,270 - Pf1N1B4_550 0.30 -2.0 647,270 - Pf1N1B4_550 0.30 -2.8 647,270 - Pf1N1B4_550 0.30 -1.6 647,270 - Pf1N1B4_550 0.30 -1.6 647,270 - Pf1N1B4_550 0.30 -0.0 647,287 + Pf1N1B4_550 0.33 -2.0 647,288 - Pf1N1B4_550 0.34 -1.0 647,288 - Pf1N1B4_550 0.34 -1.6 647,325 + -2.0 647,325 + Pf1N1B4_550 0.40 -0.0 647,325 + Pf1N1B4_550 0.40 -2.6 647,326 - Pf1N1B4_550 0.40 +2.0 647,413 - Pf1N1B4_550 0.56 -2.2 647,522 - Pf1N1B4_550 0.75 -0.2 647,662 + +0.4 647,662 + +0.6 647,662 + +1.6 647,663 - -0.0 647,714 - +0.3 647,714 - +2.6 647,714 - +1.8 647,721 - -0.0 647,836 + -2.3 647,836 + -3.3 647,836 + -0.6 647,836 + -1.3 647,836 + +0.6 647,836 + -1.6 647,836 + +0.6 647,836 + +0.3 647,836 + -2.8 647,836 + -1.6 647,836 + -1.6 647,836 + -0.0 647,836 + -0.6 647,836 + -0.4 647,836 + -1.6 647,836 + -0.7 647,837 - +1.0 647,837 - -1.0 647,837 - -0.0 647,837 - -1.3 647,837 - -1.0 647,837 - -0.0 647,837 - -0.3 647,837 - +0.5 647,837 - -1.0 647,837 - -0.0 647,837 - -1.0 647,837 - -0.6 647,837 - +1.2 647,837 - -1.0 647,837 - +2.0 647,837 - -0.0 647,837 - -1.6 647,837 - +0.5 647,837 - -0.0 647,837 - -1.0 647,837 - +2.0 647,837 - -0.0 647,837 - -2.3 647,838 + +1.6 647,838 + +1.2 647,838 + +0.1 647,838 + +0.4 647,838 + -1.0 647,838 + -2.0 647,838 + -3.0 647,839 - +0.4 647,839 - +0.2 647,839 - -0.4 647,839 - +1.0 647,839 - +1.1 647,839 - -0.0 647,842 + +0.6 647,842 + +1.0 647,842 + -1.6 647,842 + -0.0 647,842 + -0.5 647,842 + -0.0 647,842 + -2.3 647,842 + -1.3 647,842 + -1.3 647,842 + -0.3 647,843 - -0.2 647,843 - +1.0 647,843 - -2.3 647,843 - +0.4 647,843 - -1.0 647,843 - +2.0 647,843 - -1.6 647,843 - -0.6 647,845 + -0.7 647,845 + -0.0 647,845 + -1.3 647,845 + -1.0 647,846 - -1.6 647,929 + -1.0 647,930 - -0.0 647,930 - -0.0 648,068 + Pf1N1B4_551 0.17 -1.6 648,069 - Pf1N1B4_551 0.17 -2.6 648,124 + Pf1N1B4_551 0.20 +0.3 648,124 + Pf1N1B4_551 0.20 -0.8 648,124 + Pf1N1B4_551 0.20 -0.3 648,124 + Pf1N1B4_551 0.20 +0.6 648,124 + Pf1N1B4_551 0.20 -0.0 648,124 + Pf1N1B4_551 0.20 -2.0 648,124 + Pf1N1B4_551 0.20 +0.9 648,125 - Pf1N1B4_551 0.20 -2.3 648,125 - Pf1N1B4_551 0.20 +3.3 648,125 - Pf1N1B4_551 0.20 +0.4 648,125 - Pf1N1B4_551 0.20 -1.0 648,125 - +1.6 648,125 - Pf1N1B4_551 0.20 -0.0 648,125 - Pf1N1B4_551 0.20 -1.0 648,125 - Pf1N1B4_551 0.20 -1.0 648,125 - Pf1N1B4_551 0.20 -1.0 648,125 - Pf1N1B4_551 0.20 +0.2 648,125 - Pf1N1B4_551 0.20 +1.0 648,125 - Pf1N1B4_551 0.20 -1.6 648,125 - Pf1N1B4_551 0.20 -0.2 648,125 - Pf1N1B4_551 0.20 -1.1 648,353 - -0.0 648,355 + Pf1N1B4_551 0.33 -1.6 648,355 + Pf1N1B4_551 0.33 -0.0 648,355 + Pf1N1B4_551 0.33 -0.0 648,355 + Pf1N1B4_551 0.33 +1.6 648,355 + Pf1N1B4_551 0.33 -0.3 648,356 - Pf1N1B4_551 0.33 +0.8 648,356 - Pf1N1B4_551 0.33 +2.3 648,356 - Pf1N1B4_551 0.33 -0.0 648,436 + Pf1N1B4_551 0.37 -0.0 648,436 + Pf1N1B4_551 0.37 -1.0 648,490 + Pf1N1B4_551 0.40 +1.0 648,491 - Pf1N1B4_551 0.40 -0.4
Or see this region's nucleotide sequence