Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5249 and Pf1N1B4_5250 are separated by 353 nucleotides Pf1N1B4_5250 and Pf1N1B4_5251 are separated by 522 nucleotides
Pf1N1B4_5249 - Outer membrane protein A precursor, at 7,856 to 8,512
_5249
Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994
_5250
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Position (kb)
8
9 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 7.926 kb on - strand, within Pf1N1B4_5249 at 7.986 kb on + strand, within Pf1N1B4_5249 at 7.993 kb on - strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.228 kb on - strand, within Pf1N1B4_5249 at 8.289 kb on - strand, within Pf1N1B4_5249 at 8.343 kb on + strand, within Pf1N1B4_5249 at 8.428 kb on - strand, within Pf1N1B4_5249 at 8.594 kb on + strand at 8.595 kb on - strand at 8.595 kb on - strand at 8.642 kb on + strand at 8.643 kb on - strand at 8.654 kb on + strand at 8.655 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.676 kb on - strand at 8.761 kb on + strand at 8.761 kb on + strand at 8.814 kb on + strand at 8.818 kb on + strand at 8.819 kb on - strand at 8.819 kb on - strand at 9.008 kb on - strand at 9.074 kb on + strand at 9.074 kb on + strand at 9.075 kb on - strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.410 kb on + strand at 9.410 kb on + strand at 9.411 kb on - strand at 9.411 kb on - strand at 9.417 kb on + strand at 9.417 kb on + strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.422 kb on - strand at 9.422 kb on - strand at 9.518 kb on + strand at 9.518 kb on + strand at 9.519 kb on - strand at 9.558 kb on + strand at 9.558 kb on + strand at 9.596 kb on + strand at 9.597 kb on - strand at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.712 kb on + strand, within Pf1N1B4_5251 at 9.920 kb on + strand at 9.920 kb on + strand, within Pf1N1B4_5251
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 7,926 - Pf1N1B4_5249 0.11 +0.4 7,986 + Pf1N1B4_5249 0.20 -1.1 7,993 - Pf1N1B4_5249 0.21 -1.1 8,219 + Pf1N1B4_5249 0.55 -1.7 8,219 + Pf1N1B4_5249 0.55 -2.1 8,220 - Pf1N1B4_5249 0.55 +2.5 8,220 - Pf1N1B4_5249 0.55 -1.7 8,220 - Pf1N1B4_5249 0.55 -2.1 8,228 - Pf1N1B4_5249 0.57 -0.1 8,289 - Pf1N1B4_5249 0.66 -0.1 8,343 + Pf1N1B4_5249 0.74 -1.5 8,428 - Pf1N1B4_5249 0.87 -1.9 8,594 + -0.4 8,595 - +0.3 8,595 - +1.3 8,642 + -0.4 8,643 - -2.1 8,654 + -0.1 8,655 - +0.5 8,659 - -1.1 8,659 - -2.1 8,659 - -3.2 8,659 - -0.5 8,659 - -0.1 8,676 - +0.8 8,761 + +1.5 8,761 + -1.1 8,814 + +0.3 8,818 + -1.1 8,819 - -1.3 8,819 - +1.2 9,008 - +0.7 9,074 + +0.3 9,074 + -1.4 9,075 - -0.7 9,406 + -2.1 9,406 + -1.7 9,406 + -2.1 9,406 + +0.9 9,406 + -0.4 9,407 - -0.1 9,407 - -1.4 9,407 - +0.6 9,407 - +0.3 9,410 + -0.9 9,410 + -1.7 9,411 - -1.3 9,411 - +1.5 9,417 + +0.7 9,417 + -1.7 9,418 - +0.9 9,418 - -0.8 9,418 - +0.3 9,418 - -2.2 9,418 - -1.4 9,418 - +0.5 9,418 - +1.4 9,418 - -1.1 9,418 - -1.1 9,418 - +2.8 9,418 - -0.4 9,422 - +0.0 9,422 - -0.1 9,518 + +1.3 9,518 + +0.7 9,519 - -0.8 9,558 + +0.5 9,558 + +1.4 9,596 + +1.1 9,597 - +0.5 9,640 - Pf1N1B4_5251 0.14 -2.4 9,640 - Pf1N1B4_5251 0.14 +0.3 9,640 - Pf1N1B4_5251 0.14 -2.1 9,640 - Pf1N1B4_5251 0.14 -1.1 9,640 - Pf1N1B4_5251 0.14 +0.8 9,640 - Pf1N1B4_5251 0.14 +0.3 9,712 + Pf1N1B4_5251 0.23 -0.4 9,920 + -1.1 9,920 + Pf1N1B4_5251 0.47 -0.1
Or see this region's nucleotide sequence