Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_511

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_510 and Pf1N1B4_511 are separated by 60 nucleotidesPf1N1B4_511 and Pf1N1B4_512 are separated by 105 nucleotides Pf1N1B4_510 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 601,686 to 603,065 _510 Pf1N1B4_511 - hypothetical protein, at 603,126 to 603,254 _511 Pf1N1B4_512 - Short-chain dehydrogenase/reductase SDR, at 603,360 to 604,121 _512 Position (kb) 603 604Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 602.158 kb on + strand, within Pf1N1B4_510at 602.158 kb on + strand, within Pf1N1B4_510at 602.158 kb on + strand, within Pf1N1B4_510at 602.159 kb on - strandat 602.159 kb on - strand, within Pf1N1B4_510at 602.159 kb on - strand, within Pf1N1B4_510at 602.159 kb on - strand, within Pf1N1B4_510at 602.159 kb on - strand, within Pf1N1B4_510at 602.159 kb on - strand, within Pf1N1B4_510at 602.324 kb on - strand, within Pf1N1B4_510at 602.357 kb on - strand, within Pf1N1B4_510at 602.357 kb on - strand, within Pf1N1B4_510at 602.357 kb on - strand, within Pf1N1B4_510at 602.515 kb on - strand, within Pf1N1B4_510at 602.644 kb on + strand, within Pf1N1B4_510at 602.644 kb on + strand, within Pf1N1B4_510at 602.645 kb on - strand, within Pf1N1B4_510at 602.685 kb on + strandat 602.685 kb on + strand, within Pf1N1B4_510at 603.159 kb on - strand, within Pf1N1B4_511at 603.212 kb on + strand, within Pf1N1B4_511at 603.212 kb on + strand, within Pf1N1B4_511at 603.213 kb on - strand, within Pf1N1B4_511at 603.213 kb on - strand, within Pf1N1B4_511at 603.223 kb on + strand, within Pf1N1B4_511at 603.224 kb on - strand, within Pf1N1B4_511at 603.224 kb on - strand, within Pf1N1B4_511at 603.224 kb on - strand, within Pf1N1B4_511at 603.224 kb on - strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.325 kb on + strandat 603.433 kb on + strandat 603.433 kb on + strandat 603.433 kb on + strandat 603.433 kb on + strandat 603.433 kb on + strandat 603.527 kb on - strand, within Pf1N1B4_512at 603.527 kb on - strand, within Pf1N1B4_512at 603.652 kb on + strand, within Pf1N1B4_512at 603.653 kb on - strand, within Pf1N1B4_512at 603.653 kb on - strand, within Pf1N1B4_512at 603.653 kb on - strand, within Pf1N1B4_512at 603.746 kb on - strand, within Pf1N1B4_512at 603.746 kb on - strand, within Pf1N1B4_512at 603.862 kb on + strand, within Pf1N1B4_512at 603.863 kb on - strandat 603.863 kb on - strand, within Pf1N1B4_512at 603.878 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 603.882 kb on - strand, within Pf1N1B4_512at 604.125 kb on + strandat 604.125 kb on + strandat 604.125 kb on + strandat 604.126 kb on - strandat 604.126 kb on - strandat 604.126 kb on - strandat 604.126 kb on - strandat 604.170 kb on + strandat 604.171 kb on - strandat 604.238 kb on + strandat 604.238 kb on + strandat 604.238 kb on + strandat 604.239 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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602,158 + Pf1N1B4_510 0.34 -1.0
602,158 + Pf1N1B4_510 0.34 -1.0
602,158 + Pf1N1B4_510 0.34 -2.0
602,159 - -1.6
602,159 - Pf1N1B4_510 0.34 -0.5
602,159 - Pf1N1B4_510 0.34 -1.0
602,159 - Pf1N1B4_510 0.34 +0.9
602,159 - Pf1N1B4_510 0.34 +0.6
602,159 - Pf1N1B4_510 0.34 -0.4
602,324 - Pf1N1B4_510 0.46 -0.3
602,357 - Pf1N1B4_510 0.49 -1.0
602,357 - Pf1N1B4_510 0.49 -0.1
602,357 - Pf1N1B4_510 0.49 +1.0
602,515 - Pf1N1B4_510 0.60 -1.0
602,644 + Pf1N1B4_510 0.69 -1.0
602,644 + Pf1N1B4_510 0.69 -0.2
602,645 - Pf1N1B4_510 0.69 -1.0
602,685 + +1.0
602,685 + Pf1N1B4_510 0.72 -0.9
603,159 - Pf1N1B4_511 0.26 -1.6
603,212 + Pf1N1B4_511 0.67 -1.0
603,212 + Pf1N1B4_511 0.67 -2.0
603,213 - Pf1N1B4_511 0.67 -2.3
603,213 - Pf1N1B4_511 0.67 -2.8
603,223 + Pf1N1B4_511 0.75 -2.3
603,224 - Pf1N1B4_511 0.76 -3.0
603,224 - Pf1N1B4_511 0.76 +0.2
603,224 - Pf1N1B4_511 0.76 +0.2
603,224 - -1.6
603,325 + +0.2
603,325 + -1.3
603,325 + -1.6
603,325 + -0.3
603,325 + +0.3
603,325 + -0.4
603,325 + +0.8
603,433 + +0.3
603,433 + -0.6
603,433 + +1.0
603,433 + +1.2
603,433 + +1.0
603,527 - Pf1N1B4_512 0.22 -2.0
603,527 - Pf1N1B4_512 0.22 -2.6
603,652 + Pf1N1B4_512 0.38 -0.4
603,653 - Pf1N1B4_512 0.38 +2.3
603,653 - Pf1N1B4_512 0.38 -1.0
603,653 - Pf1N1B4_512 0.38 -0.8
603,746 - Pf1N1B4_512 0.51 -0.0
603,746 - Pf1N1B4_512 0.51 +0.3
603,862 + Pf1N1B4_512 0.66 +1.0
603,863 - -1.0
603,863 - Pf1N1B4_512 0.66 -1.0
603,878 - Pf1N1B4_512 0.68 +0.4
603,882 - Pf1N1B4_512 0.69 -1.0
603,882 - Pf1N1B4_512 0.69 -2.0
603,882 - Pf1N1B4_512 0.69 -0.4
603,882 - Pf1N1B4_512 0.69 -1.4
603,882 - Pf1N1B4_512 0.69 +0.7
603,882 - Pf1N1B4_512 0.69 +1.0
603,882 - Pf1N1B4_512 0.69 +0.7
603,882 - Pf1N1B4_512 0.69 -2.4
604,125 + +1.2
604,125 + +1.0
604,125 + -0.6
604,126 - -2.4
604,126 - +2.1
604,126 - -0.4
604,126 - -1.0
604,170 + -1.0
604,171 - +0.6
604,238 + -1.0
604,238 + +0.2
604,238 + -1.4
604,239 - -0.4

Or see this region's nucleotide sequence