Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5047 and Pf1N1B4_5048 are separated by 246 nucleotides Pf1N1B4_5048 and Pf1N1B4_5049 are separated by 127 nucleotides Pf1N1B4_5049 and Pf1N1B4_5050 are separated by 12 nucleotides Pf1N1B4_5050 and Pf1N1B4_5051 are separated by 44 nucleotides
Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507
_5047
Pf1N1B4_5048 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 5,592,754 to 5,593,680
_5048
Pf1N1B4_5049 - FIG00959942: hypothetical protein, at 5,593,808 to 5,594,326
_5049
Pf1N1B4_5050 - hypothetical protein, at 5,594,339 to 5,594,452
_5050
Pf1N1B4_5051 - Sensor protein DegS, at 5,594,497 to 5,595,681
_5051
Position (kb)
5592
5593
5594 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5591.974 kb on + strand at 5591.975 kb on - strand, within Pf1N1B4_5047 at 5592.048 kb on + strand, within Pf1N1B4_5047 at 5592.059 kb on + strand, within Pf1N1B4_5047 at 5592.071 kb on + strand, within Pf1N1B4_5047 at 5592.295 kb on + strand, within Pf1N1B4_5047 at 5592.356 kb on + strand, within Pf1N1B4_5047 at 5592.612 kb on - strand at 5592.661 kb on - strand at 5592.661 kb on - strand at 5592.865 kb on + strand, within Pf1N1B4_5048 at 5592.917 kb on - strand at 5592.917 kb on - strand, within Pf1N1B4_5048 at 5592.917 kb on - strand, within Pf1N1B4_5048 at 5593.517 kb on - strand at 5593.588 kb on + strand at 5593.905 kb on + strand, within Pf1N1B4_5049 at 5594.055 kb on + strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.056 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.248 kb on - strand at 5594.248 kb on - strand, within Pf1N1B4_5049 at 5594.450 kb on + strand at 5594.451 kb on - strand at 5594.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,591,974 + -0.8 5,591,975 - Pf1N1B4_5047 0.45 -2.1 5,592,048 + Pf1N1B4_5047 0.53 -1.0 5,592,059 + Pf1N1B4_5047 0.54 -0.7 5,592,071 + Pf1N1B4_5047 0.55 -1.8 5,592,295 + Pf1N1B4_5047 0.78 -0.2 5,592,356 + Pf1N1B4_5047 0.84 -1.5 5,592,612 - -0.8 5,592,661 - -2.8 5,592,661 - +1.1 5,592,865 + Pf1N1B4_5048 0.12 -0.8 5,592,917 - -0.8 5,592,917 - Pf1N1B4_5048 0.18 -2.3 5,592,917 - Pf1N1B4_5048 0.18 +0.6 5,593,517 - -0.8 5,593,588 + +2.2 5,593,905 + Pf1N1B4_5049 0.19 +2.6 5,594,055 + Pf1N1B4_5049 0.48 -2.1 5,594,056 - Pf1N1B4_5049 0.48 -0.8 5,594,056 - Pf1N1B4_5049 0.48 -1.3 5,594,248 - Pf1N1B4_5049 0.85 -0.3 5,594,248 - Pf1N1B4_5049 0.85 -0.3 5,594,248 - Pf1N1B4_5049 0.85 +0.6 5,594,248 - Pf1N1B4_5049 0.85 +0.8 5,594,248 - -2.3 5,594,248 - Pf1N1B4_5049 0.85 -0.8 5,594,450 + -0.5 5,594,451 - +0.9 5,594,490 + +1.2
Or see this region's nucleotide sequence