Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5038 and Pf1N1B4_5039 are separated by 190 nucleotides Pf1N1B4_5039 and Pf1N1B4_5040 are separated by 86 nucleotides Pf1N1B4_5040 and Pf1N1B4_5041 are separated by 31 nucleotides Pf1N1B4_5041 and Pf1N1B4_5042 overlap by 1 nucleotides
Pf1N1B4_5038 - Various polyols ABC transporter, periplasmic substrate-binding protein, at 5,582,893 to 5,584,203
_5038
Pf1N1B4_5039 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,584,394 to 5,585,167
_5039
Pf1N1B4_5040 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 5,585,254 to 5,585,694
_5040
Pf1N1B4_5041 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 5,585,726 to 5,586,112
_5041
Pf1N1B4_5042 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 5,586,112 to 5,588,811
_5042
Position (kb)
5584
5585
5586 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5583.787 kb on + strand, within Pf1N1B4_5038 at 5583.787 kb on + strand, within Pf1N1B4_5038 at 5583.788 kb on - strand, within Pf1N1B4_5038 at 5583.872 kb on - strand, within Pf1N1B4_5038 at 5583.872 kb on - strand, within Pf1N1B4_5038 at 5583.937 kb on + strand, within Pf1N1B4_5038 at 5583.937 kb on + strand at 5583.938 kb on - strand at 5583.949 kb on + strand, within Pf1N1B4_5038 at 5583.949 kb on + strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5584.036 kb on + strand, within Pf1N1B4_5038 at 5584.037 kb on - strand, within Pf1N1B4_5038 at 5584.052 kb on - strand at 5584.202 kb on - strand at 5584.368 kb on - strand at 5584.370 kb on - strand at 5584.376 kb on + strand at 5584.470 kb on - strand at 5584.470 kb on - strand at 5584.470 kb on - strand at 5584.557 kb on - strand at 5584.703 kb on + strand, within Pf1N1B4_5039 at 5584.744 kb on + strand, within Pf1N1B4_5039 at 5584.745 kb on - strand, within Pf1N1B4_5039 at 5584.851 kb on - strand, within Pf1N1B4_5039 at 5584.994 kb on + strand, within Pf1N1B4_5039 at 5585.043 kb on + strand, within Pf1N1B4_5039 at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.136 kb on - strand at 5585.136 kb on - strand at 5585.270 kb on + strand at 5585.270 kb on + strand at 5585.271 kb on - strand at 5585.271 kb on - strand at 5585.422 kb on - strand, within Pf1N1B4_5040 at 5585.519 kb on + strand, within Pf1N1B4_5040 at 5585.520 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.838 kb on + strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.937 kb on + strand, within Pf1N1B4_5041 at 5585.938 kb on - strand, within Pf1N1B4_5041
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,583,787 + Pf1N1B4_5038 0.68 +0.2 5,583,787 + Pf1N1B4_5038 0.68 +0.4 5,583,788 - Pf1N1B4_5038 0.68 +2.2 5,583,872 - Pf1N1B4_5038 0.75 +0.1 5,583,872 - Pf1N1B4_5038 0.75 -2.8 5,583,937 + Pf1N1B4_5038 0.80 -1.0 5,583,937 + -2.3 5,583,938 - -0.0 5,583,949 + Pf1N1B4_5038 0.81 -1.2 5,583,949 + Pf1N1B4_5038 0.81 -0.2 5,583,950 - Pf1N1B4_5038 0.81 -2.8 5,583,950 - Pf1N1B4_5038 0.81 -1.8 5,583,950 - Pf1N1B4_5038 0.81 -0.1 5,584,036 + Pf1N1B4_5038 0.87 -0.2 5,584,037 - Pf1N1B4_5038 0.87 -2.3 5,584,052 - -0.8 5,584,202 - -1.8 5,584,368 - -1.8 5,584,370 - -1.8 5,584,376 + -0.6 5,584,470 - +1.0 5,584,470 - +1.4 5,584,470 - -0.8 5,584,557 - -0.8 5,584,703 + Pf1N1B4_5039 0.40 -0.5 5,584,744 + Pf1N1B4_5039 0.45 -1.5 5,584,745 - Pf1N1B4_5039 0.45 -3.1 5,584,851 - Pf1N1B4_5039 0.59 -0.5 5,584,994 + Pf1N1B4_5039 0.78 -1.3 5,585,043 + Pf1N1B4_5039 0.84 -2.2 5,585,117 + -1.1 5,585,117 + +1.7 5,585,117 + -2.8 5,585,135 + -3.3 5,585,135 + +0.4 5,585,135 + -0.0 5,585,136 - +1.6 5,585,136 - +1.0 5,585,270 + -0.2 5,585,270 + -0.1 5,585,271 - -1.3 5,585,271 - -1.8 5,585,422 - Pf1N1B4_5040 0.38 -0.8 5,585,519 + Pf1N1B4_5040 0.60 -0.8 5,585,520 - Pf1N1B4_5040 0.60 +0.1 5,585,579 - Pf1N1B4_5040 0.74 -0.0 5,585,579 - Pf1N1B4_5040 0.74 -1.8 5,585,579 - Pf1N1B4_5040 0.74 -0.1 5,585,579 - Pf1N1B4_5040 0.74 +1.1 5,585,579 - Pf1N1B4_5040 0.74 -0.8 5,585,838 + Pf1N1B4_5041 0.29 -3.1 5,585,839 - Pf1N1B4_5041 0.29 -1.8 5,585,839 - Pf1N1B4_5041 0.29 -1.0 5,585,839 - Pf1N1B4_5041 0.29 -2.8 5,585,839 - Pf1N1B4_5041 0.29 -0.5 5,585,937 + Pf1N1B4_5041 0.55 -0.1 5,585,938 - Pf1N1B4_5041 0.55 -0.2
Or see this region's nucleotide sequence