Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4975 and Pf1N1B4_4976 are separated by 145 nucleotides Pf1N1B4_4976 and Pf1N1B4_4977 are separated by 111 nucleotides
Pf1N1B4_4975 - hypothetical protein, at 5,514,804 to 5,515,145
_4975
Pf1N1B4_4976 - D-serine/D-alanine/glycine transporter, at 5,515,291 to 5,516,712
_4976
Pf1N1B4_4977 - Ornithine cyclodeaminase (EC 4.3.1.12), at 5,516,824 to 5,517,849
_4977
Position (kb)
5515
5516
5517 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5514.363 kb on + strand at 5514.558 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.680 kb on - strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.695 kb on - strand at 5514.695 kb on - strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.815 kb on - strand at 5514.815 kb on - strand at 5514.919 kb on - strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand at 5515.042 kb on - strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.160 kb on + strand at 5515.548 kb on - strand, within Pf1N1B4_4976 at 5515.572 kb on + strand, within Pf1N1B4_4976 at 5515.573 kb on - strand, within Pf1N1B4_4976 at 5515.767 kb on + strand, within Pf1N1B4_4976 at 5515.767 kb on + strand at 5516.038 kb on + strand, within Pf1N1B4_4976 at 5516.409 kb on - strand, within Pf1N1B4_4976 at 5516.492 kb on - strand, within Pf1N1B4_4976 at 5516.547 kb on + strand at 5516.549 kb on + strand, within Pf1N1B4_4976 at 5516.554 kb on - strand, within Pf1N1B4_4976 at 5516.557 kb on + strand, within Pf1N1B4_4976 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.186 kb on + strand, within Pf1N1B4_4977 at 5517.320 kb on + strand at 5517.321 kb on - strand, within Pf1N1B4_4977 at 5517.641 kb on + strand at 5517.641 kb on + strand, within Pf1N1B4_4977 at 5517.642 kb on - strand, within Pf1N1B4_4977 at 5517.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,514,363 + +0.6 5,514,558 + +0.4 5,514,580 + -0.8 5,514,580 + -2.3 5,514,580 + -0.0 5,514,580 + -0.8 5,514,580 + -0.8 5,514,580 + -1.1 5,514,679 + +2.5 5,514,679 + -0.6 5,514,679 + -0.9 5,514,679 + -1.2 5,514,679 + +2.2 5,514,679 + +0.8 5,514,680 - -3.1 5,514,691 + +0.4 5,514,691 + +0.2 5,514,691 + +0.9 5,514,691 + +0.5 5,514,695 - -2.6 5,514,695 - -0.8 5,514,814 + +1.7 5,514,814 + -0.2 5,514,814 + +0.2 5,514,815 - -1.2 5,514,815 - -2.3 5,514,919 - Pf1N1B4_4975 0.34 +0.7 5,515,041 + Pf1N1B4_4975 0.69 -0.3 5,515,041 + Pf1N1B4_4975 0.69 -0.4 5,515,041 + -1.8 5,515,042 - Pf1N1B4_4975 0.70 -0.5 5,515,074 + Pf1N1B4_4975 0.79 -1.8 5,515,074 + Pf1N1B4_4975 0.79 -0.8 5,515,074 + +1.1 5,515,074 + Pf1N1B4_4975 0.79 +0.7 5,515,160 + +0.0 5,515,548 - Pf1N1B4_4976 0.18 -2.8 5,515,572 + Pf1N1B4_4976 0.20 -1.2 5,515,573 - Pf1N1B4_4976 0.20 -0.2 5,515,767 + Pf1N1B4_4976 0.33 -1.8 5,515,767 + -0.2 5,516,038 + Pf1N1B4_4976 0.53 -1.8 5,516,409 - Pf1N1B4_4976 0.79 -0.8 5,516,492 - Pf1N1B4_4976 0.84 +0.8 5,516,547 + -0.8 5,516,549 + Pf1N1B4_4976 0.88 +2.4 5,516,554 - Pf1N1B4_4976 0.89 +1.2 5,516,557 + Pf1N1B4_4976 0.89 +1.2 5,517,038 + Pf1N1B4_4977 0.21 -1.8 5,517,038 + Pf1N1B4_4977 0.21 +0.8 5,517,186 + Pf1N1B4_4977 0.35 +0.1 5,517,320 + -0.8 5,517,321 - Pf1N1B4_4977 0.48 -0.8 5,517,641 + -1.8 5,517,641 + Pf1N1B4_4977 0.80 -1.1 5,517,642 - Pf1N1B4_4977 0.80 -3.3 5,517,654 - +0.8
Or see this region's nucleotide sequence