Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4888 and Pf1N1B4_4889 are separated by 302 nucleotides Pf1N1B4_4889 and Pf1N1B4_4890 overlap by 4 nucleotides
Pf1N1B4_4888 - UvrD/REP helicase family protein, at 5,408,350 to 5,410,773
_4888
Pf1N1B4_4889 - Cystathionine beta-synthase (EC 4.2.1.22), at 5,411,076 to 5,412,452
_4889
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Position (kb)
5411
5412
5413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5410.088 kb on - strand, within Pf1N1B4_4888 at 5410.153 kb on + strand, within Pf1N1B4_4888 at 5410.191 kb on + strand, within Pf1N1B4_4888 at 5410.192 kb on - strand at 5410.320 kb on + strand at 5410.321 kb on - strand at 5410.454 kb on - strand, within Pf1N1B4_4888 at 5410.488 kb on - strand, within Pf1N1B4_4888 at 5410.529 kb on - strand, within Pf1N1B4_4888 at 5410.624 kb on + strand at 5410.624 kb on + strand at 5410.625 kb on - strand at 5410.625 kb on - strand at 5410.664 kb on - strand at 5410.695 kb on + strand at 5411.055 kb on + strand at 5411.055 kb on + strand at 5411.056 kb on - strand at 5411.457 kb on + strand, within Pf1N1B4_4889 at 5411.458 kb on - strand, within Pf1N1B4_4889 at 5411.461 kb on - strand, within Pf1N1B4_4889 at 5411.558 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.666 kb on - strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.015 kb on - strand, within Pf1N1B4_4889 at 5412.407 kb on + strand at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.570 kb on - strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand at 5413.067 kb on + strand at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.302 kb on - strand, within Pf1N1B4_4890
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,410,088 - Pf1N1B4_4888 0.72 -0.8 5,410,153 + Pf1N1B4_4888 0.74 -0.5 5,410,191 + Pf1N1B4_4888 0.76 -0.1 5,410,192 - +1.2 5,410,320 + -1.8 5,410,321 - +0.2 5,410,454 - Pf1N1B4_4888 0.87 +0.6 5,410,488 - Pf1N1B4_4888 0.88 +0.5 5,410,529 - Pf1N1B4_4888 0.90 +0.0 5,410,624 + -0.5 5,410,624 + -0.8 5,410,625 - -0.1 5,410,625 - -0.0 5,410,664 - -2.8 5,410,695 + -0.2 5,411,055 + +0.6 5,411,055 + -2.4 5,411,056 - +0.8 5,411,457 + Pf1N1B4_4889 0.28 -3.8 5,411,458 - Pf1N1B4_4889 0.28 -1.8 5,411,461 - Pf1N1B4_4889 0.28 +0.6 5,411,558 + Pf1N1B4_4889 0.35 -0.0 5,411,665 + Pf1N1B4_4889 0.43 +1.6 5,411,665 + Pf1N1B4_4889 0.43 -0.2 5,411,666 - Pf1N1B4_4889 0.43 +0.2 5,411,721 + Pf1N1B4_4889 0.47 +0.4 5,411,721 + Pf1N1B4_4889 0.47 -1.8 5,411,909 + Pf1N1B4_4889 0.60 +0.5 5,411,909 + Pf1N1B4_4889 0.60 -0.8 5,412,013 - Pf1N1B4_4889 0.68 -0.2 5,412,013 - Pf1N1B4_4889 0.68 -1.8 5,412,013 - Pf1N1B4_4889 0.68 -1.8 5,412,013 - Pf1N1B4_4889 0.68 -0.8 5,412,015 - Pf1N1B4_4889 0.68 -0.8 5,412,407 + +0.2 5,412,569 + Pf1N1B4_4890 0.10 -1.8 5,412,569 + Pf1N1B4_4890 0.10 +0.6 5,412,570 - Pf1N1B4_4890 0.10 -0.2 5,412,955 + Pf1N1B4_4890 0.43 -0.8 5,412,955 + Pf1N1B4_4890 0.43 -1.2 5,413,067 + -1.8 5,413,067 + +0.2 5,413,067 + Pf1N1B4_4890 0.52 -0.8 5,413,067 + Pf1N1B4_4890 0.52 -1.8 5,413,067 + Pf1N1B4_4890 0.52 -1.0 5,413,068 - Pf1N1B4_4890 0.52 +1.2 5,413,068 - Pf1N1B4_4890 0.52 +0.6 5,413,302 - Pf1N1B4_4890 0.72 -2.1
Or see this region's nucleotide sequence