Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_482 and Pf1N1B4_483 are separated by 135 nucleotides Pf1N1B4_483 and Pf1N1B4_484 are separated by 92 nucleotides Pf1N1B4_484 and Pf1N1B4_485 are separated by 84 nucleotides
Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822
_482
Pf1N1B4_483 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 574,958 to 575,539
_483
Pf1N1B4_484 - Hydrogenase maturation factor, at 575,632 to 576,402
_484
Pf1N1B4_485 - L-lysine permease, at 576,487 to 577,041
_485
Position (kb)
574
575
576 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 574.083 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.727 kb on + strand at 574.727 kb on + strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.882 kb on - strand at 574.888 kb on - strand at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.452 kb on - strand, within Pf1N1B4_483 at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.626 kb on - strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.670 kb on - strand at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.822 kb on - strand, within Pf1N1B4_484 at 575.857 kb on + strand, within Pf1N1B4_484 at 575.857 kb on + strand, within Pf1N1B4_484 at 575.857 kb on + strand, within Pf1N1B4_484 at 575.912 kb on - strand, within Pf1N1B4_484 at 575.912 kb on - strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.001 kb on + strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.002 kb on - strand, within Pf1N1B4_484 at 576.127 kb on + strand, within Pf1N1B4_484 at 576.127 kb on + strand, within Pf1N1B4_484 at 576.239 kb on + strand, within Pf1N1B4_484 at 576.239 kb on + strand at 576.240 kb on - strand, within Pf1N1B4_484 at 576.416 kb on + strand at 576.416 kb on + strand at 576.416 kb on + strand at 576.417 kb on - strand at 576.417 kb on - strand at 576.521 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 574,083 + Pf1N1B4_482 0.26 -0.6 574,233 + Pf1N1B4_482 0.41 +1.0 574,233 + Pf1N1B4_482 0.41 +0.5 574,234 - Pf1N1B4_482 0.41 -1.6 574,234 - Pf1N1B4_482 0.41 +0.7 574,237 + Pf1N1B4_482 0.41 +0.6 574,237 + Pf1N1B4_482 0.41 +1.3 574,238 - Pf1N1B4_482 0.41 -0.0 574,238 - Pf1N1B4_482 0.41 +2.0 574,238 - Pf1N1B4_482 0.41 -0.8 574,238 - Pf1N1B4_482 0.41 -0.0 574,238 - Pf1N1B4_482 0.41 +0.6 574,238 - Pf1N1B4_482 0.41 -1.6 574,238 - Pf1N1B4_482 0.41 +1.0 574,238 - Pf1N1B4_482 0.41 -2.3 574,313 + Pf1N1B4_482 0.49 +0.5 574,313 + Pf1N1B4_482 0.49 -0.2 574,314 - Pf1N1B4_482 0.49 +0.9 574,314 - Pf1N1B4_482 0.49 +0.2 574,314 - Pf1N1B4_482 0.49 +0.6 574,727 + +1.0 574,727 + +1.0 574,728 - -0.0 574,728 - -1.6 574,728 - -1.0 574,882 - -1.0 574,888 - +0.2 575,451 + Pf1N1B4_483 0.85 +0.2 575,451 + Pf1N1B4_483 0.85 +1.3 575,451 + Pf1N1B4_483 0.85 -0.0 575,451 + Pf1N1B4_483 0.85 +1.2 575,452 - Pf1N1B4_483 0.85 +1.3 575,526 - -0.6 575,526 - -0.6 575,526 - -0.0 575,526 - +2.2 575,615 + +0.3 575,615 + -2.0 575,615 + -0.3 575,625 + -1.0 575,625 + -1.0 575,625 + -1.8 575,626 - +3.2 575,669 + -0.0 575,669 + -2.3 575,669 + -0.7 575,670 - +1.0 575,779 + Pf1N1B4_484 0.19 +2.8 575,779 + Pf1N1B4_484 0.19 +1.5 575,779 + Pf1N1B4_484 0.19 -0.2 575,779 + Pf1N1B4_484 0.19 -0.4 575,779 + Pf1N1B4_484 0.19 -3.7 575,780 - Pf1N1B4_484 0.19 +0.7 575,780 - Pf1N1B4_484 0.19 +1.0 575,780 - Pf1N1B4_484 0.19 -0.0 575,805 + Pf1N1B4_484 0.22 +0.4 575,805 + Pf1N1B4_484 0.22 -1.2 575,821 + Pf1N1B4_484 0.25 +0.5 575,821 + Pf1N1B4_484 0.25 -1.0 575,821 + Pf1N1B4_484 0.25 -1.0 575,822 - Pf1N1B4_484 0.25 -1.6 575,857 + Pf1N1B4_484 0.29 -1.0 575,857 + Pf1N1B4_484 0.29 -2.0 575,857 + Pf1N1B4_484 0.29 +1.0 575,912 - Pf1N1B4_484 0.36 -1.6 575,912 - Pf1N1B4_484 0.36 +1.3 576,001 + Pf1N1B4_484 0.48 -1.0 576,001 + Pf1N1B4_484 0.48 -2.0 576,001 + Pf1N1B4_484 0.48 -0.0 576,001 + Pf1N1B4_484 0.48 +1.1 576,001 + Pf1N1B4_484 0.48 -2.0 576,001 + Pf1N1B4_484 0.48 -1.0 576,001 + -1.6 576,001 + Pf1N1B4_484 0.48 -1.8 576,001 + Pf1N1B4_484 0.48 +2.3 576,001 + Pf1N1B4_484 0.48 -2.5 576,001 + Pf1N1B4_484 0.48 +0.8 576,001 + Pf1N1B4_484 0.48 -0.0 576,001 + Pf1N1B4_484 0.48 +1.0 576,001 + Pf1N1B4_484 0.48 -0.7 576,001 + Pf1N1B4_484 0.48 -2.8 576,001 + Pf1N1B4_484 0.48 -1.0 576,001 + Pf1N1B4_484 0.48 -0.0 576,001 + Pf1N1B4_484 0.48 -2.0 576,001 + Pf1N1B4_484 0.48 +0.2 576,001 + Pf1N1B4_484 0.48 -1.0 576,001 + Pf1N1B4_484 0.48 -1.8 576,001 + Pf1N1B4_484 0.48 -1.0 576,001 + Pf1N1B4_484 0.48 -2.0 576,001 + Pf1N1B4_484 0.48 -0.0 576,002 - Pf1N1B4_484 0.48 -1.2 576,002 - Pf1N1B4_484 0.48 +0.7 576,002 - Pf1N1B4_484 0.48 +2.3 576,002 - Pf1N1B4_484 0.48 -0.0 576,002 - Pf1N1B4_484 0.48 -0.0 576,002 - Pf1N1B4_484 0.48 -1.0 576,002 - Pf1N1B4_484 0.48 -0.4 576,002 - Pf1N1B4_484 0.48 -0.8 576,002 - Pf1N1B4_484 0.48 +0.7 576,002 - Pf1N1B4_484 0.48 +0.3 576,002 - Pf1N1B4_484 0.48 -1.6 576,002 - Pf1N1B4_484 0.48 -0.7 576,002 - Pf1N1B4_484 0.48 -3.3 576,002 - Pf1N1B4_484 0.48 -0.0 576,127 + Pf1N1B4_484 0.64 +0.6 576,127 + Pf1N1B4_484 0.64 +0.4 576,239 + Pf1N1B4_484 0.79 +0.1 576,239 + -0.0 576,240 - Pf1N1B4_484 0.79 +1.0 576,416 + -0.0 576,416 + +0.2 576,416 + -0.0 576,417 - -2.8 576,417 - -0.0 576,521 + +0.8
Or see this region's nucleotide sequence