Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4784 and Pf1N1B4_4785 are separated by 16 nucleotides Pf1N1B4_4785 and Pf1N1B4_4786 are separated by 12 nucleotides Pf1N1B4_4786 and Pf1N1B4_4787 are separated by 165 nucleotides
Pf1N1B4_4784 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 5,285,831 to 5,287,495
_4784
Pf1N1B4_4785 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 5,287,512 to 5,288,273
_4785
Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479
_4786
Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,289,645 to 5,290,772
_4787
Position (kb)
5288
5289
5290 Strain fitness (log2 ratio)
-2
-1
0
1 at 5287.432 kb on + strand at 5288.182 kb on - strand, within Pf1N1B4_4785 at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.206 kb on + strand at 5288.206 kb on + strand at 5288.271 kb on + strand at 5288.272 kb on - strand at 5288.272 kb on - strand at 5288.281 kb on + strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.312 kb on + strand at 5288.313 kb on - strand at 5288.313 kb on - strand at 5288.313 kb on - strand at 5289.036 kb on - strand at 5289.474 kb on + strand at 5289.474 kb on + strand at 5289.617 kb on + strand at 5289.761 kb on + strand at 5289.762 kb on - strand, within Pf1N1B4_4787 at 5289.917 kb on - strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.215 kb on + strand, within Pf1N1B4_4787 at 5290.218 kb on - strand, within Pf1N1B4_4787 at 5290.218 kb on - strand at 5290.262 kb on + strand, within Pf1N1B4_4787
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,287,432 + -0.1 5,288,182 - Pf1N1B4_4785 0.88 +1.2 5,288,202 - +0.4 5,288,202 - -1.4 5,288,202 - -0.8 5,288,202 - +0.7 5,288,206 + -1.8 5,288,206 + -0.8 5,288,271 + +1.5 5,288,272 - -0.4 5,288,272 - +0.6 5,288,281 + +0.5 5,288,282 - +0.3 5,288,282 - +0.8 5,288,282 - -0.4 5,288,312 + -2.5 5,288,313 - -1.4 5,288,313 - -2.4 5,288,313 - -1.5 5,289,036 - -0.8 5,289,474 + +0.1 5,289,474 + -0.9 5,289,617 + -1.0 5,289,761 + -1.8 5,289,762 - Pf1N1B4_4787 0.10 +1.2 5,289,917 - Pf1N1B4_4787 0.24 -1.1 5,290,080 + Pf1N1B4_4787 0.39 -0.2 5,290,080 + Pf1N1B4_4787 0.39 -1.8 5,290,215 + Pf1N1B4_4787 0.51 +1.5 5,290,218 - Pf1N1B4_4787 0.51 -0.8 5,290,218 - -1.8 5,290,262 + Pf1N1B4_4787 0.55 +0.5
Or see this region's nucleotide sequence