Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4772 and Pf1N1B4_4773 are separated by 15 nucleotides Pf1N1B4_4773 and Pf1N1B4_4774 are separated by 292 nucleotides Pf1N1B4_4774 and Pf1N1B4_4775 are separated by 4 nucleotides
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Pf1N1B4_4773 - RND efflux system, membrane fusion protein CmeA, at 5,274,456 to 5,275,613
_4773
Pf1N1B4_4774 - Transcriptional regulatory protein RstA, at 5,275,906 to 5,276,607
_4774
Pf1N1B4_4775 - Sensory histidine kinase QseC, at 5,276,612 to 5,277,913
_4775
Position (kb) 5274
5275
5276 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5273.476 kb on + strand, within Pf1N1B4_4772 at 5273.477 kb on - strand, within Pf1N1B4_4772 at 5273.745 kb on - strand, within Pf1N1B4_4772 at 5273.988 kb on + strand, within Pf1N1B4_4772 at 5275.615 kb on + strand at 5275.616 kb on - strand at 5275.616 kb on - strand at 5275.616 kb on - strand at 5275.731 kb on + strand at 5275.731 kb on + strand at 5275.732 kb on - strand at 5275.732 kb on - strand at 5275.733 kb on + strand at 5275.768 kb on + strand at 5275.768 kb on + strand at 5275.812 kb on + strand at 5276.383 kb on + strand, within Pf1N1B4_4774 at 5276.383 kb on + strand, within Pf1N1B4_4774 at 5276.384 kb on - strand, within Pf1N1B4_4774 at 5276.424 kb on - strand, within Pf1N1B4_4774 at 5276.424 kb on - strand, within Pf1N1B4_4774
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,273,476 + Pf1N1B4_4772 0.69 +1.7 5,273,477 - Pf1N1B4_4772 0.69 +2.4 5,273,745 - Pf1N1B4_4772 0.78 +0.9 5,273,988 + Pf1N1B4_4772 0.85 +3.2 5,275,615 + +1.7 5,275,616 - +2.7 5,275,616 - +0.2 5,275,616 - +1.4 5,275,731 + -0.8 5,275,731 + -0.8 5,275,732 - -2.8 5,275,732 - -3.1 5,275,733 + +1.5 5,275,768 + +0.8 5,275,768 + +3.2 5,275,812 + -3.4 5,276,383 + Pf1N1B4_4774 0.68 -2.4 5,276,383 + Pf1N1B4_4774 0.68 -0.8 5,276,384 - Pf1N1B4_4774 0.68 -2.8 5,276,424 - Pf1N1B4_4774 0.74 -2.4 5,276,424 - Pf1N1B4_4774 0.74 -1.4
Or see this region's nucleotide sequence